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AB270210

Human Thrombin ELISA Kit

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(2 Publications)

Human Thrombin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thrombin with a sensitivity of 55 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Prothrombin, Coagulation factor II, F2

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ELISA - Human Thrombin ELISA Kit (AB270210)
  • ELISA

Supplier Data

ELISA - Human Thrombin ELISA Kit (AB270210)
Sandwich ELISA - Human Thrombin ELISA Kit (AB270210)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombin ELISA Kit (AB270210)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombin concentration was determined to be 68 μg/mL with a range of 35 – 117 μg/mL.

Sandwich ELISA - Human Thrombin ELISA Kit (AB270210)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombin ELISA Kit (AB270210)

Example of human Thrombin standard curve in Sample Diluent NS +1X Enhancer.

The Thrombin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Thrombin ELISA Kit (AB270210)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombin ELISA Kit (AB270210)

Interpolated concentrations of native Thrombin in human serum and plasma samples.

The concentrations of Thrombin were measured in duplicates, interpolated from the Thrombin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 20,000, plasma (citrate) 1 : 10,000, plasma (EDTA) 1 : 10,000, and plasma (heparin) 1 : 10,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombin concentration was determined to be 56 μg/mL in serum, 34 μg/mL in plasma (citrate), 37 μg/mL in plasma (EDTA), and 31 μg/mL in plasma (heparin).

Sandwich ELISA - Human Thrombin ELISA Kit (AB270210)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombin ELISA Kit (AB270210)

Representative image of Human Thrombin ELISA Kit (ab270210)

Components shown from left to right :

- Sample diluent NS

- 10X Human Thrombin Capture Antibody

- 10X Human Thrombin Detector Antibody

- Human Thrombin Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 55 pg/mL

Range

140.63 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Thrombin ELISA Kit ab270210 is a rapid single-wash 90-min Sandwich ELISA to measure Human Thrombin in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 55 pg/mL.

How the assay works

Human Thrombin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Thrombin ELISA Kit ab270210 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Thrombin antibody [EPR20159-201] - BSA and Azide free (Capture) ab272288
- Anti-Thrombin antibody [EPR20159-45] - BSA and Azide free (Detector) ab272288

Thrombin (activated Factor II [IIa]) is a coagulation protein that has many effects in the coagulation cascade. Thrombin is a serine protease that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions. Thrombin is in the form of alpha-thrombin that is the immediate end-product of prothrombin activation: two further thrombin products can be identified, beta- and gamma-thrombin. These are degraded forms that may arise from autodigestion of a thrombin preparation. Human Thrombin shares 81%, sequence homology with mouse and bovine as well as 80% with rat.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 99 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "93 - 99 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "96 - 101 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "90 - 92 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "91 - 93 %", "average": "= 92" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab270210-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB270210-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270210-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB270210-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270210-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB270210-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB270210-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Thrombin Lyophilized Recombinant Protein", "number":"AB270210-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB270210-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Thrombin Capture Antibody", "number":"AB270210-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270210-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Thrombin Detector Antibody", "number":"AB270210-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombin also known as Factor II or thrombin protein is a 36 kDa serine protease essential for blood coagulation. It is produced in the liver as prothrombin and activated in the coagulation cascade. Its expression occurs extensively in the liver where it plays an important role in converting soluble fibrinogen into insoluble fibrin forming blood clots. Thrombin also activates various other coagulation factors including Factors V VIII and XI amplifying the coagulation response. Biotinylated thrombin variations help in detection and research applications.
Biological function summary

Thrombin modulates several physiological processes beyond clot formation. It serves as a signaling molecule interacting with protease-activated receptors (PARs) to influence cell functions including proliferation migration and apoptosis. Thrombin forms part of the prothrombinase complex comprised of prothrombin activated Factor X (Xa) and Factor V on phospholipid surfaces. This complex is critical for thrombin generation during the clotting cascade. Thrombin's activity extends to involvement in wound healing and inflammation regulation.

Pathways

Thrombin significantly participates in the coagulation and fibrinolytic pathways. It not only converts fibrinogen to fibrin in the coagulation pathway but also activates inhibitors like antithrombin which regulates thrombin and other protease activities. Thrombin's interaction with fibrinolysis where tPA and plasminogen are substrates integrates clot disintegration processes. Thrombin's connectivity with proteins such as Factor VII Protein C and antithrombin outlines its diverse role in maintaining hemostatic balance.

Thrombin associates with conditions such as venous thromboembolism and disseminated intravascular coagulation (DIC). Dysregulation of thrombin leads to excessive clotting contributing to thromboembolic diseases. In DIC uncontrolled thrombin activity results in systemic coagulation consuming clotting factors and increasing bleeding risk. Its function alongside proteins like Factor V Leiden and antithrombin deficiencies aggravate thrombotic conditions. Research into thrombin inhibitors aids in developing therapeutic strategies for these disorders.
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Product protocols

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Target data

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (PubMed : 2019570, PubMed : 21976677). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (PubMed : 30568593, PubMed : 9780208).
See full target information F2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 11: PubMed36556051

2022

Correlation of Biomarkers of Endothelial Injury and Inflammation to Outcome in Hospitalized COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Levy Munguía,Nayelli Nájera,Felipe de Jesús Martínez,Dylan Díaz-Chiguer,Fiacro Jiménez-Ponce,Miguel Ortiz-Flores,Francisco Villarreal,Guillermo Ceballos

Bioengineered 13:4939-4950 PubMed35156527

2022

Quantitative proteomics reveal three potential biomarkers for risk assessment of acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Xie,Hongzhou Zhang,Tieqiu Huang
View all publications
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websiteProtocolBooklet
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