AB327318
Human Thrombin ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- What is this?
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Human Thrombin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Thrombin with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 96.02 - 70000 pg/ml with a sensitivity of 38.47 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 96.02 - 70000 pg/ml with a sensitivity of 38.47 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
4 Images
- sELISA
Supplier Data
Sandwich ELISA - Human Thrombin ELISA Kit, Chemiluminescent (AB327318)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Thrombin concentration was determined to be 68 µg/mL with a range of 35 – 117 µg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Thrombin ELISA Kit, Chemiluminescent (AB327318)
Interpolated concentrations of native Thrombin in human serum and plasma samples.
The concentrations of Thrombin were measured in duplicates interpolated from the Thrombin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 20000 plasma (citrate) 1 : 10000 plasma (EDTA) 1 : 10000 and plasma (heparin) 1 : 10000. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Thrombin concentration was determined to be 56 µg/mL in serum 34 µg/mL in plasma (citrate) 37 µg/mL in plasma (EDTA) and 31 µg/mL in plasma (heparin).
- sELISA
Lab
Sandwich ELISA - Human Thrombin ELISA Kit, Chemiluminescent (AB327318)
Standard curve comparison between the Chemiluminescent Human Thrombin SimpleStep ELISA and colorimetric Human Thrombin SimpleStep ELISA (ab270210) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Human Thrombin ELISA Kit, Chemiluminescent (AB327318)
Dynamic range comparison between the Chemiluminescent Human Thrombin SimpleStep ELISA and colorimetric Human Thrombin SimpleStep ELISA (ab270210). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.
Reactivity data
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Product details
Human Thrombin ELISA Kit, Chemiluminescent (ab327318) is a rapid single-wash sandwich 55-min ELISA to measure Human Thrombin in Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma with a sensitivity of 38.47 pg/mL.
How the assay works
Human Thrombin ELISA Kit, Chemiluminescent (ab327318) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Thrombin (ab327318) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Thrombin antibody [EPR20159-201] - BSA and Aside free (Capture) ab272287
- Anti-Thrombin antibody [EPR20159-45] - BSA and Aside free (Detector) ab272288
How the assay works
Human Thrombin ELISA Kit, Chemiluminescent (ab327318) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Thrombin (ab327318) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Thrombin antibody [EPR20159-201] - BSA and Aside free (Capture) ab272287
- Anti-Thrombin antibody [EPR20159-45] - BSA and Aside free (Detector) ab272288
Precision
[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
Recovery
[ { "sample": "Serum", "range": "91 - 99 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "93 - 99 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "96 - 101 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "90 - 92 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "91 - 93 %", "average": "= 92" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (PubMed : 2019570, PubMed : 21976677). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (PubMed : 30568593, PubMed : 9780208).
Alternative Names
Prothrombin, Coagulation factor II, F2
Product promise
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