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AB108909

Human Thrombin ELISA Kit (Factor II)

4

(1 Review)

|

(8 Publications)

Human Thrombin ELISA Kit (Factor II) is a Sandwich ELISA for the measurement of Human Thrombin (Factor II) in Human in Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

Prothrombin, Coagulation factor II, F2

1 Images
Sandwich ELISA - Human Thrombin ELISA Kit (Factor II) (AB108909)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombin ELISA Kit (Factor II) (AB108909)

Representative Standard Curve Using ab108909.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Tissue, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 71 pg/mL

Range

0.313 - 20 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Thrombin (Factor II) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin concentrations in cell culture supernatants, cell lysate and tissue samples. This assay recognizes both natural and recombinant Human thrombin.

A Thrombin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108909-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108909-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB108909-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108909-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108909-CMP03", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108909-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108909-CMP05", "productcode":"" }, { "size":"1 x 60 µL", "name":"100X Biotinylated Human Thrombin Antibody", "number":"AB108909-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Thrombin Standard", "number":"AB108909-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Thrombin Microplate (12 x 8 well strips)", "number":"AB108909-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombin also known as Factor II or thrombin protein is a 36 kDa serine protease essential for blood coagulation. It is produced in the liver as prothrombin and activated in the coagulation cascade. Its expression occurs extensively in the liver where it plays an important role in converting soluble fibrinogen into insoluble fibrin forming blood clots. Thrombin also activates various other coagulation factors including Factors V VIII and XI amplifying the coagulation response. Biotinylated thrombin variations help in detection and research applications.
Biological function summary

Thrombin modulates several physiological processes beyond clot formation. It serves as a signaling molecule interacting with protease-activated receptors (PARs) to influence cell functions including proliferation migration and apoptosis. Thrombin forms part of the prothrombinase complex comprised of prothrombin activated Factor X (Xa) and Factor V on phospholipid surfaces. This complex is critical for thrombin generation during the clotting cascade. Thrombin's activity extends to involvement in wound healing and inflammation regulation.

Pathways

Thrombin significantly participates in the coagulation and fibrinolytic pathways. It not only converts fibrinogen to fibrin in the coagulation pathway but also activates inhibitors like antithrombin which regulates thrombin and other protease activities. Thrombin's interaction with fibrinolysis where tPA and plasminogen are substrates integrates clot disintegration processes. Thrombin's connectivity with proteins such as Factor VII Protein C and antithrombin outlines its diverse role in maintaining hemostatic balance.

Thrombin associates with conditions such as venous thromboembolism and disseminated intravascular coagulation (DIC). Dysregulation of thrombin leads to excessive clotting contributing to thromboembolic diseases. In DIC uncontrolled thrombin activity results in systemic coagulation consuming clotting factors and increasing bleeding risk. Its function alongside proteins like Factor V Leiden and antithrombin deficiencies aggravate thrombotic conditions. Research into thrombin inhibitors aids in developing therapeutic strategies for these disorders.
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Product protocols

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Target data

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (PubMed : 2019570, PubMed : 21976677). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (PubMed : 30568593, PubMed : 9780208).
See full target information F2
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35563479

2022

Engineering CRISPR/Cas9 for Multiplexed Recombinant Coagulation Factor Production.

Applications

Unspecified application

Species

Unspecified reactive species

Colby J Feser,Christopher J Lees,Daniel T Lammers,Megan J Riddle,Jason R Bingham,Matthew J Eckert,Jakub Tolar,Mark J Osborn

Critical care medicine 50:e31-e39 PubMed34369427

2021

Dielectric Blood Coagulometry for the Early Detection of Sepsis-Induced Disseminated Intravascular Coagulation: A Prospective Observational Study.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Takayama,Akira Endo,Koji Morishita,Yasuhiro Otomo

Theranostics 11:4251-4261 PubMed33754059

2021

Dabigatran activates inflammation resolution by promoting fibrinogen-like protein 2 shedding and RvD5 production.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Lei,Yu Zhou,Huakan Zhao,Yu Chen,Guifang Yan,Lei Wu,Yanquan Xu,Jiangang Zhang,Xiao Zhang,Jingchun Wang,Dingshan Li,Yongsheng Li

Transplantation 103:1821-1833 PubMed30964836

2019

Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Laura J Zitur,Peter J Chlebeck,Scott K Odorico,Juan S Danobeitia,Tiffany J Zens,Cees Van Kooten,Michael Eerhart,Jose A Reyes,Megan L Springer,Jennifer M Coonen,Kevin G Brunner,Saverio V Capuano,Anthony M D'Alessandro,Luis A Fernandez

eLife 7: PubMed29889025

2018

N-glycosylation in the protease domain of trypsin-like serine proteases mediates calnexin-assisted protein folding.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Shuo Li,Juejin Wang,Shenghan Chen,Xue-Long Sun,Qingyu Wu

British journal of clinical pharmacology 83:2661-2670 PubMed28735510

2017

FXa inhibition by rivaroxaban modifies mechanisms associated with the pathogenesis of human abdominal aortic aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Guillermo Moñux,Jose J Zamorano-León,Pablo Marqués,Bernardo Sopeña,J M García-García,G Laich de Koller,Bibiana Calvo-Rico,Miguel A García-Fernandez,J Serrano,Antonio López-Farré

Current eye research 41:1590-1600 PubMed27261371

2016

Upregulation of Thrombin/Matrix Metalloproteinase-1/Protease-Activated Receptor-1 Chain in Proliferative Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Kaiser Alam,Mohd Imtiaz Nawaz,Ghulam Mohammad,Kathleen Van den Eynde,Mohammad Mairaj Siddiquei,Ahmed Mousa,Gert De Hertogh,Ghislain Opdenakker

Arthritis research & therapy 14:R91 PubMed22541814

2012

Thrombin induces heme oxygenase-1 expression in human synovial fibroblasts through protease-activated receptor signaling pathways.

Applications

sELISA

Species

Human

Ju-Fang Liu,Sheng-Mou Hou,Chun-Hao Tsai,Chun-Yin Huang,Wei-Hung Yang,Chih-Hsin Tang
View all publications
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Product promise

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