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AB214029

Human Thrombomodulin ELISA Kit

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(5 Publications)

Human Thrombomodulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thrombomodulin with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin

10 Images
ELISA - Human Thrombomodulin ELISA Kit (AB214029)
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Supplier Data

ELISA - Human Thrombomodulin ELISA Kit (AB214029)
Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)
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Supplier Data

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)

Example of Human Thrombomodulin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)

Interpolated concentrations of native Thrombomodulin in HUVEC and human liver homogenate extracts based on a 1,000 μg/mL extract load.

The concentrations of Thrombomodulin were measured in duplicate and interpolated from the Thrombomodulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 1.2 ng/mL in HUVEC extract and 8.0 ng/mL in liver homogenate extract.

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)
  • sELISA

Lab

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)

Human Thrombomodulin standard curve comparison data.

Standard curve comparison between human Thrombomodulin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 8-fold increase in sensitivity.

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 5.1 ng/mL with a range of 2.7 – 9.9 ng/mL.

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)

Example of Human Thrombomodulin standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)

Interpolated concentrations of spiked recombinant Thrombomodulin in HepG2 cell culture supernatant samples.

The concentrations of Thrombomodulin were measured in duplicate, interpolated from the Thrombomodulin standard curve and corrected for sample dilution. Undiluted sample is at 45%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The native Thrombomodulin concentration was undetectable in the null spike control run in parallel with the spiked sample.

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)

Interpolated concentrations of native Thrombomodulin in human serum, and plasma samples.

The concentrations of Thrombomodulin were measured in duplicate, interpolated from the Thrombomodulin standard curve and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 4.8 ng/mL in serum, 3.9 ng/mL in plasma (citrate), 3.8 ng/mL in plasma (EDTA) and 4.3 ng/mL in plasma (heparin).

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)

Representative image of Human Thrombomodulin ELISA Kit (ab214029)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Human Thrombomodulin Detector Antibody

- Human Thrombomodulin Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Human Thrombomodulin Capture Antibody (Lyophilised)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombomodulin ELISA Kit (AB214029)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 5.1 ng/mL with a range of 3.6 – 6.8 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3 pg/mL

Range

20.3 - 1300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Thrombomodulin ELISA Kit ab214029 is a rapid single-wash 90-min Sandwich ELISA to measure Human Thrombomodulin in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 3 pg/mL.

How the assay works

Human Thrombomodulin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Thrombomodulin ELISA Kit ab214029 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Thrombomodulin antibody [EPR19229-167] - BSA and Azide free (Capture) ab242992
- Anti-Thrombomodulin antibody [EPR19230-10] - BSA and Azide free (Detector) ab242992

Human Thrombomodulin is a 557 amino acid type I transmembrane protein, consisting of six epidermal growth factor (EGF)-like domains and is highly glycosylated. Thrombomodulin is an important component in the anti-coagulation system by functioning as a cell surface receptor for thrombin, and has also been shown to play a role in anti-inflammation, cell adhesion, and proliferation. The Thrombomodulin - thrombin complex activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI); protein C degrades coagulation factors Va and VIIIa, which regulates thrombin resulting in inhibition of coagulation. The extracellular portion of Thrombomodulin has been shown to be released from the cell surface due to protein cleavage during vascular endothelial cell injury.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 91 %", "average": "= 90" }, { "sample": "Serum", "range": "96 - 99 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Cell culture extracts", "range": "92 - 96 %", "average": "= 94" }, { "sample": "Urine", "range": "102 - 112 %", "average": "= 106" }, { "sample": "Tissue Extracts", "range": "101 - 110 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "98 - 100 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "92 - 98 %", "average": "= 96" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab214029-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB214029-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB214029-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB214029-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB214029-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB214029-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB214029-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB214029-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Thrombomodulin Capture Antibody (Lyophilized)", "number":"AB214029-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Thrombomodulin Lyophilized Recombinant Protein (ab98989)", "number":"AB214029-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB214029-CMP07", "productcode":"" }, { "size":"1 x 300 µL", "name":"20X Human Thrombomodulin Detector Antibody", "number":"AB214029-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB214029-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.
Biological function summary

Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.

Pathways

Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.

Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.
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Product protocols

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Target data

Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed : 10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed : 29323190, PubMed : 33836597, PubMed : 9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed : 26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed : 15841214).
See full target information THBD
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:12414 PubMed40216867

2025

Liver quad culture chip as a model for radiation injury research.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Ueda,Satoshi Omiya,Jonathan Pinney,Michelle A Bylicky,Molykutty J Aryankalayil

The Tohoku journal of experimental medicine : PubMed39721678

2024

Metformin Mitigates Bevacizumab-Evoked Endothelial Dysfunction through Activating MEK/ERK and PI3K/AKT Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liu,Ruoyu Deng,Zhenzhen Yang,Dong Zhou

Scientific reports 12:21935 PubMed36536028

2022

Elevated endothelial dysfunction-related biomarker levels indicate the severity and predict sepsis incidence.

Applications

Unspecified application

Species

Unspecified reactive species

Gaosheng Zhou,Jingjing Liu,Hongmin Zhang,Xiaoting Wang,Dawei Liu

European journal of heart failure 23:1916-1926 PubMed34415085

2021

Association of COVID-19 with impaired endothelial glycocalyx, vascular function and myocardial deformation 4 months after infection.

Applications

Unspecified application

Species

Unspecified reactive species

Vaia Lambadiari,Asimina Mitrakou,Aikaterini Kountouri,John Thymis,Konstantinos Katogiannis,Emmanouil Korakas,Charalampos Varlamos,Ioanna Andreadou,Maria Tsoumani,Helen Triantafyllidi,Aristotelis Bamias,Konstantinos Thomas,Pinelopi Kazakou,Sotiria Grigoropoulou,Dimitra Kavatha,Anastasia Antoniadou,Meletios-Athanasios Dimopoulos,Ignatios Ikonomidis

Neurology(R) neuroimmunology & neuroinflammation 6:e531 PubMed30697583

2019

HIV is associated with endothelial activation despite ART, in a sub-Saharan African setting.

Applications

ELISA

Species

Unspecified reactive species

Joseph Kamtchum-Tatuene,Henry Mwandumba,Zaid Al-Bayati,Janet Flatley,Michael Griffiths,Tom Solomon,Laura Benjamin
View all publications
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websiteProtocolBooklet
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