Human Thrombomodulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thrombomodulin with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Scientific reports 12:219352022Elevated endothelial dysfunction-related biomarker levels indicate the severity and predict sepsis incidence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaosheng Zhou,Jingjing Liu,Hongmin Zhang,Xiaoting Wang,Dawei LiuPubMed 36536028
- European journal of heart failure 23:1916-19262021Association of COVID-19 with impaired endothelial glycocalyx, vascular function and myocardial deformation 4 months after infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVaia Lambadiari,Asimina Mitrakou,Aikaterini Kountouri,John Thymis,Konstantinos Katogiannis,Emmanouil Korakas,Charalampos Varlamos,Ioanna Andreadou,Maria Tsoumani,Helen Triantafyllidi,Aristotelis Bamias,Konstantinos Thomas,Pinelopi Kazakou,Sotiria Grigoropoulou,Dimitra Kavatha,Anastasia Antoniadou,Meletios-Athanasios Dimopoulos,Ignatios IkonomidisPubMed 34415085
- Neurology(R) neuroimmunology & neuroinflammation 6:e5312019HIV is associated with endothelial activation despite ART, in a sub-Saharan African setting.Applications:ELISAReactive species:Unspecified reactive speciesJoseph Kamtchum-Tatuene,Henry Mwandumba,Zaid Al-Bayati,Janet Flatley,Michael Griffiths,Tom Solomon,Laura BenjaminPubMed 30697583
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 20.3 - 1300 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed:10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed:29323190, PubMed:33836597, PubMed:9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed:26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed:15841214).
Alternative names
CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin
What's included?
Recommended products
Human Thrombomodulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thrombomodulin with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 20.3 - 1300 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Human Serum | n 8 | C.V. 3.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Human Serum | n 3 | C.V. 9.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 90 | Range 88 - 91 % |
Sample type Serum | Average % = 97 | Range 96 - 99 % |
Sample type EDTA Plasma | Average % = 96 | Range 94 - 98 % |
Sample type Cell culture extracts | Average % = 94 | Range 92 - 96 % |
Sample type Urine | Average % = 106 | Range 102 - 112 % |
Sample type Tissue Extracts | Average % = 105 | Range 101 - 110 % |
Sample type Heparin Plasma | Average % = 99 | Range 98 - 100 % |
Sample type Citrate plasma | Average % = 96 | Range 92 - 98 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Thrombomodulin ELISA Kit (ab214029) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombomodulin protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thrombomodulin with 3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Thrombomodulin is a 557 amino acid type I transmembrane protein, consisting of six epidermal growth factor (EGF)-like domains and is highly glycosylated. Thrombomodulin is an important component in the anti-coagulation system by functioning as a cell surface receptor for thrombin, and has also been shown to play a role in anti-inflammation, cell adhesion, and proliferation. The Thrombomodulin - thrombin complex activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI); protein C degrades coagulation factors Va and VIIIa, which regulates thrombin resulting in inhibition of coagulation. The extracellular portion of Thrombomodulin has been shown to be released from the cell surface due to protein cleavage during vascular endothelial cell injury.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.
- Biological function summary
Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.
- Pathways
Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.
- Associated diseases and disorders
Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.
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8 product images
Sandwich ELISA - Human Thrombomodulin ELISA Kit (ab214029)
Human Thrombomodulin standard curve comparison data.
Standard curve comparison between human Thrombomodulin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 8-fold increase in sensitivity.
Sandwich ELISA - Human Thrombomodulin ELISA Kit (ab214029)
Example of Human Thrombomodulin standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Thrombomodulin ELISA Kit (ab214029)
Example of Human Thrombomodulin standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Thrombomodulin ELISA Kit (ab214029)
Interpolated concentrations of native Thrombomodulin in human serum, and plasma samples.
The concentrations of Thrombomodulin were measured in duplicate, interpolated from the Thrombomodulin standard curve and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 4.8 ng/mL in serum, 3.9 ng/mL in plasma (citrate), 3.8 ng/mL in plasma (EDTA) and 4.3 ng/mL in plasma (heparin).
Sandwich ELISA - Human Thrombomodulin ELISA Kit (ab214029)
Interpolated concentrations of spiked recombinant Thrombomodulin in HepG2 cell culture supernatant samples.
The concentrations of Thrombomodulin were measured in duplicate, interpolated from the Thrombomodulin standard curve and corrected for sample dilution. Undiluted sample is at 45%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The native Thrombomodulin concentration was undetectable in the null spike control run in parallel with the spiked sample.
Sandwich ELISA - Human Thrombomodulin ELISA Kit (ab214029)
Interpolated concentrations of native Thrombomodulin in HUVEC and human liver homogenate extracts based on a 1,000 μg/mL extract load.
The concentrations of Thrombomodulin were measured in duplicate and interpolated from the Thrombomodulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 1.2 ng/mL in HUVEC extract and 8.0 ng/mL in liver homogenate extract.
Sandwich ELISA - Human Thrombomodulin ELISA Kit (ab214029)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 5.1 ng/mL with a range of 3.6 – 6.8 ng/mL.
Sandwich ELISA - Human Thrombomodulin ELISA Kit (ab214029)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 5.1 ng/mL with a range of 2.7 – 9.9 ng/mL.
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