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AB46508

Human Thrombomodulin ELISA Kit (CD141)

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(1 Review)

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(21 Publications)

Human Thrombomodulin ELISA Kit (CD141) is a Sandwich (quantitative) ELISA for the measurement of Human Thrombomodulin (CD141) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin

2 Images
Sandwich ELISA - Human Thrombomodulin ELISA Kit (CD141) (AB46508)
  • sELISA

Unknown

Sandwich ELISA - Human Thrombomodulin ELISA Kit (CD141) (AB46508)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Sandwich ELISA - Human Thrombomodulin ELISA Kit (CD141) (AB46508)
  • sELISA

Unknown

Sandwich ELISA - Human Thrombomodulin ELISA Kit (CD141) (AB46508)

Thrombomodulin measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 2-16 fold.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.31 ng/mL

Range

0.625 - 20 ng/mL

Assay time

1h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Thrombomodulin (CD141) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombomodulin (CD141) in serum, plasma, buffered solutions and supernatants.

A monoclonal antibody specific for Thrombomodulin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Thrombomodulin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Thrombomodulin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Thrombomodulin present in the samples.

This kit will recognize both endogenous and recombinant Human Thrombomodulin.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46508-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46508-CMP03" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46508-CMP10" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46508-CMP09" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46508-CMP08" }, { "size":"1 x 1 Unit", "name":"Thrombomodulin Microplate (12 x 8 well strips)", "number":"AB46508-CMP06" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46508-CMP02" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46508-CMP11" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46508-CMP05" }, { "size":"2 x 1 Vial", "name":"Thrombomodulin Standard (lyophilized)", "number":"AB46508-CMP07" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-Thrombomodulin", "number":"AB46508-CMP04" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46508-CMP01" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46508-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46508-CMP03", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46508-CMP08", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46508-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46508-CMP10", "productcode":"" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46508-CMP05", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-Thrombomodulin", "number":"AB46508-CMP04", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46508-CMP11", "productcode":"" }, { "size":"4 x 1 Vial", "name":"Thrombomodulin Standard (lyophilized)", "number":"AB46508-CMP07", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46508-CMP09", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46508-CMP01", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Thrombomodulin Microplate (12 x 8 well strips)", "number":"AB46508-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.
Biological function summary

Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.

Pathways

Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.

Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.
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Product protocols

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Target data

Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed : 10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed : 29323190, PubMed : 33836597, PubMed : 9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed : 26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed : 15841214).
See full target information THBD
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Brain communications 7:fcaf070 PubMed40008326

2025

Structural brain changes in post-COVID condition and its relationship with cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Pacheco-Jaime,Carla Garcia-Vicente,Mar Ariza,Neus Cano,Maite Garolera,Lourdes Carreras-Vidal,Ignacio Roura,Clara Capdevila-Lacasa,Javier Oltra,Jèssica Pardo,Cristina Martín-Barceló,Anna Campabadal,Roser Sala-Llonch,Núria Bargalló,Cristian Barrué,Javier Bejar,Claudio U Cortés,Carme Junqué,Bàrbara Segura

Frontiers in pharmacology 15:1407825 PubMed39257391

2024

Developing a predictive nomogram for mortality in patients with extrapulmonary acute respiratory distress syndrome: the prognostic value of serum soluble thrombomodulin, lung ultrasound score, and lactate.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Yue Wang,Guoguo Zhu,Siya Xu,Jie Liu,Zhongzhi Tang

Blood advances 8:5663-5673 PubMed39231312

2024

Plasmin generation analysis in patients with bleeding disorder of unknown cause.

Applications

Unspecified application

Species

Unspecified reactive species

Dino Mehic,Stéphanie E Reitsma,Claire de Moreuil,Helmuth Haslacher,Maximilian C Koeller,Bas de Laat,Cihan Ay,Ingrid Pabinger,Alisa S Wolberg,Johanna Gebhart

Kidney international reports 7:797-809 PubMed35497798

2022

Minimal Change Disease Is Associated With Endothelial Glycocalyx Degradation and Endothelial Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Colin Bauer,Federica Piani,Mindy Banks,Flor A Ordoñez,Carmen de Lucas-Collantes,Kaori Oshima,Eric P Schmidt,Igor Zakharevich,Alfons Segarra,Cristina Martinez,Carlos Roncal-Jimenez,Simon C Satchell,Petter Bjornstad,Marshall Scott Lucia,Judith Blaine,Joshua M Thurman,Richard J Johnson,Gabriel Cara-Fuentes

Haemophilia : the official journal of the World Federation of Hemophilia 27:1028-1036 PubMed34628704

2021

Thrombomodulin in patients with mild to moderate bleeding tendency.

Applications

Unspecified application

Species

Unspecified reactive species

Dino Mehic,Alexander Tolios,Stefanie Hofer,Cihan Ay,Helmuth Haslacher,Kate Downes,Matthias Haimel,Ingrid Pabinger,Johanna Gebhart

TH open : companion journal to thrombosis and haemostasis 5:e253-e263 PubMed34235394

2021

Endothelium Activation Markers in Severe Hospitalized COVID-19 Patients: Role in Mortality Risk Prediction.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Marchetti,Patricia Gomez-Rosas,Eleonora Sanga,Sara Gamba,Cristina Verzeroli,Laura Russo,Francesco Restuccia,Francesca Schieppati,Ezio Bonanomi,Marco Rizzi,Stefano Fagiuoli,Andrea D'Alessio,Luca Lorini,Anna Falanga

bioRxiv : the preprint server for biology : PubMed33655244

2021

Increased complement activation is a distinctive feature of severe SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Ma,Sanjaya K Sahu,Marlene Cano,Vasanthan Kuppuswamy,Jamal Bajwa,Ja'Nia McPhatter,Alexander Pine,Matthew Meizlish,George Goshua,C-Hong Chang,Hanming Zhang,Christina Price,Parveen Bahel,Henry Rinder,Tingting Lei,Aaron Day,Daniel Reynolds,Xiaobo Wu,Rebecca Schriefer,Adriana M Rauseo,Charles W Goss,Jane A O'Halloran,Rachel M Presti,Alfred H Kim,Andrew E Gelman,Charles Dela Cruz,Alfred I Lee,Phillip Mudd,Hyung J Chun,John P Atkinson,Hrishikesh S Kulkarni

Journal of internal medicine 289:738-746 PubMed33511686

2021

Tocilizumab in COVID-19 interstitial pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

G Pomponio,A Ferrarini,M Bonifazi,M Moretti,A Salvi,A Giacometti,M Tavio,G Titolo,L Morbidoni,G Frausini,M Onesta,D Amico,M L B Rocchi,S Menzo,L Zuccatosta,F Mei,V Menditto,S Svegliati,A Donati,M M D'Errico,M Pavani,A Gabrielli

The Lancet. Haematology 7:e575-e582 PubMed32619411

2020

Endotheliopathy in COVID-19-associated coagulopathy: evidence from a single-centre, cross-sectional study.

Applications

Unspecified application

Species

Unspecified reactive species

George Goshua,Alexander B Pine,Matthew L Meizlish,C-Hong Chang,Hanming Zhang,Parveen Bahel,Audrey Baluha,Noffar Bar,Robert D Bona,Adrienne J Burns,Charles S Dela Cruz,Anne Dumont,Stephanie Halene,John Hwa,Jonathan Koff,Hope Menninger,Natalia Neparidze,Christina Price,Jonathan M Siner,Christopher Tormey,Henry M Rinder,Hyung J Chun,Alfred I Lee

Diabetes, metabolic syndrome and obesity : targets 13:1599-1607 PubMed32494176

2020

Low Vitamin D Serum Level Is Associated with HDL-C Dyslipidemia and Increased Serum Thrombomodulin Levels of Insulin-Resistant Individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Saheb Sharif-Askari,Narjes Saheb Sharif-Askari,Rabih Halwani,Salah Abusnana,Rifat Hamoudi,Nabil Sulaiman
View all publications
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