AB219632

Human Thrombopoietin ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Human Thrombopoietin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thrombopoietin with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MGDF, THPO, Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML

7 Images

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

sELISA

Supplier Data

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Example of human Thrombopoietin standard curve in Sample Diluent 10BP.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Interpolated concentrations of native Thrombopoietin in human serum and plasma samples.

The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum neat, plasma (citrate) neat, plasma (heparin) neat, plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable Thrombopoietin concentration was determined to be 47.1 pg/mL in neat serum, 79.6 pg/mL in neat plasma (citrate), 109 pg/mL in plasma (heparin), and 54.1 pg/mL in plasma (EDTA). Detectable concentrations are defined as concentrations above the minimum detectable dose (MDD) concentration.

sELISA

Supplier Data

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Serum from ten individual healthy female human donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable Thrombopoietin concentration was determined to be 91.1 pg/mL with a range of 56.0 – 151 pg/mL. Detectable concentrations are defined as concentrations above the minimum detectable dose (MDD) concentration.

sELISA

Supplier Data

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Example of human Thrombopoietin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Interpolated concentrations of spike Thrombopoietin in human plasma samples.

sELISA

Supplier Data

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Interpolated concentrations of spike Thrombopoietin in human serum and cell culture media (DMEM + 10% FBS) samples.

sELISA

Supplier Data

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Example of human Thrombopoietin standard curve in Sample Diluent 25BP.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 36 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Thrombopoietin ELISA Kit (ab219632) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombopoietin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thrombopoietin with 11 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Thrombopoietin is a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. Thrombopoietin acts at a late stage of megakaryocyte development.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]

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Recovery

[ { "sample": "Serum", "range": "81 - 84 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "96 - 109 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "97 - 100 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "80 - 90 %", "average": "= 84" }, { "sample": "Cell culture media", "range": "84 - 84 %", "average": "= 89" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab219632-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB219632-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB219632-CMP07", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB219632-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB219632-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB219632-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Thrombopoietin Lyophilized Recombinant Protein", "number":"AB219632-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BP", "number":"AB219632-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB219632-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Thrombopoietin Capture Antibody", "number":"AB219632-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB219632-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Thrombopoietin Detector Antibody", "number":"AB219632-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombopoietin also known as TPO is an important glycoprotein hormone with a mass of about 70 kDa. It is primarily produced in the liver but can also be found in the kidneys and bone marrow stromal cells. Thrombopoietin binds to and activates its receptor c-Mpl which is present on hematopoietic progenitor cells. This protein has a significant role in regulating the production of blood platelets by stimulating the proliferation and differentiation of megakaryocytes the precursor cells for platelets.

Biological function summary

Thrombopoietin functions in hematopoiesis by supporting the maturation of megakaryocytes. It operates independently but can interact with other factors to optimize platelet production. Although it does not form a part of a large protein complex it is essential for the proper function of hematopoietic progenitors across several stages of differentiation. Its activity ensures the maintenance of platelet levels in the bloodstream therefore preventing conditions like thrombocytopenia.

Pathways

Thrombopoietin plays a critical role in the megakaryocyte/platelet lineage pathway. It directly influences the JAK-STAT (Janus kinase-signal transducer and activator of transcription) signaling pathway which is vital for driving the cell cycle and promoting cell survival. Thrombopoietin shares a functional connection with EPO (erythropoietin) which is involved in red blood cell production although they act on different lineage-specific progenitors.

Thrombopoietin is intricately linked to conditions like thrombocytopenia where there are abnormally low platelet counts and thrombocythemia characterized by elevated platelet levels. Dysregulation of Thrombopoietin production or its receptor activity can lead to these disorders. It has a relationship with other hematopoietic growth factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which can also influence the disorders of blood cell lines. Understanding its signaling pathways provides insights into therapeutic targets for diseases involving platelet imbalances.

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Product protocols

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Target data

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

See full target information THPO

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 56:1327-1334 PubMed38553338

2024

Reticulated platelets are increased and hyper-activated in patients with cirrhosis, especially those with poor outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Zanetto,Serena Toffanin,Elena Campello,Claudia Maria Radu,Sabrina Gavasso,Patrizia Burra,Francesco Paolo Russo,Marco Senzolo,Paolo Simioni

International journal of cardiology. Heart & vascu 23:100346 PubMed30976651

2019

Reduction in thrombogenic activity and thrombocytopenia after transcatheter aortic valve implantation - The ATTRACTIVE-TTAS study.

Applications

Unspecified application

Species

Unspecified reactive species

Masanobu Ishii,Koichi Kaikita,Tatsuro Mitsuse,Nobuhiro Nakanishi,Yu Oimatsu,Takayoshi Yamashita,Suguru Nagamatsu,Noriaki Tabata,Koichiro Fujisue,Daisuke Sueta,Seiji Takashio,Yuichiro Arima,Kenji Sakamoto,Eiichiro Yamamoto,Kenichi Tsujita

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Thrombopoietin ELISA Kit

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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