Human Thrombopoietin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thrombopoietin with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Publications
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- International journal of cardiology. Heart & vascu 23:1003462019Reduction in thrombogenic activity and thrombocytopenia after transcatheter aortic valve implantation - The ATTRACTIVE-TTAS study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasanobu Ishii et. al.PubMed 30976651
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 36 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Alternative names
MGDF, THPO, Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML
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Human Thrombopoietin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thrombopoietin with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 36 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 83 | Range 81 - 84 % |
Sample type EDTA Plasma | Average % = 101 | Range 96 - 109 % |
Sample type Heparin Plasma | Average % = 98 | Range 97 - 100 % |
Sample type Citrate plasma | Average % = 84 | Range 80 - 90 % |
Sample type Cell culture media | Average % = 89 | Range 84 - 84 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Thrombopoietin ELISA Kit (ab219632) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombopoietin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thrombopoietin with 11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Thrombopoietin is a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. Thrombopoietin acts at a late stage of megakaryocyte development.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thrombopoietin also known as TPO is an important glycoprotein hormone with a mass of about 70 kDa. It is primarily produced in the liver but can also be found in the kidneys and bone marrow stromal cells. Thrombopoietin binds to and activates its receptor c-Mpl which is present on hematopoietic progenitor cells. This protein has a significant role in regulating the production of blood platelets by stimulating the proliferation and differentiation of megakaryocytes the precursor cells for platelets.
- Biological function summary
Thrombopoietin functions in hematopoiesis by supporting the maturation of megakaryocytes. It operates independently but can interact with other factors to optimize platelet production. Although it does not form a part of a large protein complex it is essential for the proper function of hematopoietic progenitors across several stages of differentiation. Its activity ensures the maintenance of platelet levels in the bloodstream therefore preventing conditions like thrombocytopenia.
- Pathways
Thrombopoietin plays a critical role in the megakaryocyte/platelet lineage pathway. It directly influences the JAK-STAT (Janus kinase-signal transducer and activator of transcription) signaling pathway which is vital for driving the cell cycle and promoting cell survival. Thrombopoietin shares a functional connection with EPO (erythropoietin) which is involved in red blood cell production although they act on different lineage-specific progenitors.
- Associated diseases and disorders
Thrombopoietin is intricately linked to conditions like thrombocytopenia where there are abnormally low platelet counts and thrombocythemia characterized by elevated platelet levels. Dysregulation of Thrombopoietin production or its receptor activity can lead to these disorders. It has a relationship with other hematopoietic growth factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which can also influence the disorders of blood cell lines. Understanding its signaling pathways provides insights into therapeutic targets for diseases involving platelet imbalances.
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7 product images
Sandwich ELISA - Human Thrombopoietin ELISA Kit (ab219632)
Interpolated concentrations of spike Thrombopoietin in human plasma samples.
The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) neat, plasma (heparin) neat, plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Thrombopoietin ELISA Kit (ab219632)
Interpolated concentrations of spike Thrombopoietin in human serum and cell culture media (DMEM + 10% FBS) samples.
The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, cell culture media neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Thrombopoietin ELISA Kit (ab219632)
Interpolated concentrations of native Thrombopoietin in human serum and plasma samples.
The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (heparin) neat, plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable Thrombopoietin concentration was determined to be 47.1 pg/mL in neat serum, 79.6 pg/mL in neat plasma (citrate), 109 pg/mL in plasma (heparin), and 54.1 pg/mL in plasma (EDTA). Detectable concentrations are defined as concentrations above the minimum detectable dose (MDD) concentration.
Sandwich ELISA - Human Thrombopoietin ELISA Kit (ab219632)
Serum from ten individual healthy female human donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable Thrombopoietin concentration was determined to be 91.1 pg/mL with a range of 56.0 – 151 pg/mL. Detectable concentrations are defined as concentrations above the minimum detectable dose (MDD) concentration.
Sandwich ELISA - Human Thrombopoietin ELISA Kit (ab219632)
Example of human Thrombopoietin standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Thrombopoietin ELISA Kit (ab219632)
Example of human Thrombopoietin standard curve in Sample Diluent 10BP.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Thrombopoietin ELISA Kit (ab219632)
Example of human Thrombopoietin standard curve in Sample Diluent 25BP.
Background-subtracted data values (mean +/- SD) are graphed.
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