Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Thymidine phosphorylase (PD-ECGF) - Intracellular in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 23.438 - 1500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.32 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
Alternative names
ECGF1, TYMP, Thymidine phosphorylase, TP, Gliostatin, Platelet-derived endothelial cell growth factor, TdRPase, PD-ECGF
What's included?
Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Thymidine phosphorylase (PD-ECGF) - Intracellular in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 23.438 - 1500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7.32 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. = 7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. = 2.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 95 | Range 91 - 97 % |
Sample type Tissue Extracts | Average % = 95 | Range 92 - 97 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Thymidine phosphorylase (PD-ECGF) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thymidine phosphorylase (PD-ECGF) protein in Cell Lysate, Tissue extracts. Quantitate Human Thymidine phosphorylase (PD-ECGF) with 7.32 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Singlewash protocol reduces assay time to 90 minutes or less.
- High sensitivity, specificity and reproducibility from superior antibodies.
- Fully validated in biological samples.
- 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).
- Also available in a fully validated 384well format.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular (ab324132)
Example of human Thymidine Phosphorylase standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular (ab324132)
Interpolated concentration of native Thymidine Phosphorylase was measured in duplicate at different sample concentrations. Undiluted samples are as follows: A-431 cell extract 1.25 ug/mL, HeLa cell extract 6.25 ug/mL, spleen tissue extract 2 ug/mL, and kidney tissue extract 6.25 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular (ab324132)
Example of human Thymidine Phosphorylase standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular (ab324132)
Interpolated concentration of native Thymidine Phosphorylase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are spleen tissue extract 0.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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