AB324132

Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular

Recombinant
SimpleStep
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Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular is a Sandwich ELISA for the measurement of Human Thymidine phosphorylase (PD-ECGF) - Intracellular in Human in Cell/Tissue Extracts samples.

View Alternative Names

ECGF1, TYMP, Thymidine phosphorylase, TP, Gliostatin, Platelet-derived endothelial cell growth factor, TdRPase, PD-ECGF

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Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular (AB324132)

sELISA

Supplier Data

Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular (AB324132)

Example of human Thymidine Phosphorylase standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular (AB324132)

Interpolated concentration of native Thymidine Phosphorylase was measured in duplicate at different sample concentrations. Undiluted samples are as follows : A-431 cell extract 1.25 ug/mL, HeLa cell extract 6.25 ug/mL, spleen tissue extract 2 ug/mL, and kidney tissue extract 6.25 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular (AB324132)

Interpolated concentration of native Thymidine Phosphorylase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are spleen tissue extract 0.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Intracellular (AB324132)

Example of human Thymidine Phosphorylase standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.32 pg/mL

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Thymidine phosphorylase (PD-ECGF) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thymidine phosphorylase (PD-ECGF) protein in Cell Lysate, Tissue extracts. Quantitate Human Thymidine phosphorylase (PD-ECGF) with 7.32 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Singlewash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).
Also available in a fully validated 384well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.2" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "91 - 97 %", "average": "= 95" }, { "sample": "Tissue Extracts", "range": "92 - 97 %", "average": "= 95" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.. Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

See full target information TYMP

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websiteProtocolBooklet

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Product promise

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