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AB155441

Human Thyroglobulin ELISA Kit

5

(1 Review)

|

(2 Publications)

Human Thyroglobulin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Thyroglobulin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Thyroglobulin, Tg, TG

1 Images
Sandwich ELISA - Human Thyroglobulin ELISA Kit (AB155441)
  • sELISA

Unknown

Sandwich ELISA - Human Thyroglobulin ELISA Kit (AB155441)

Representative standard curve using ab155441 - Assay Diluent C.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.22 ng/mL

Range

0.205 - 50 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Thyroglobulin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Thyroglobulin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Thyroglobulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thyroglobulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Thyroglobulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thyroglobulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "105 - 125 %", "average": "= 115.5" }, { "sample": "Plasma", "range": "88 - 106 %", "average": "= 98.71" }, { "sample": "Cell culture media", "range": "88 - 103 %", "average": "= 96.01" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155441-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155441-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155441-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Thyroglobulin Microplate (12 x 8 wells)", "number":"AB155441-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Thyroglobulin", "number":"AB155441-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Thyroglobulin Standard (lyophilized)", "number":"AB155441-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155441-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155441-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155441-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB155441-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thyroglobulin often abbreviated as Tg is a glycoprotein with a molecular mass of approximately 660 kDa. It is expressed primarily in the thyroid gland particularly within the follicular cells. This protein serves as a precursor for thyroid hormones such as thyroxine (T4) and triiodothyronine (T3). High thyroglobulin levels in the bloodstream can indicate altered thyroid function and clinicians frequently measure these levels for diagnostic purposes. Researchers have developed specific anti-thyroglobulin antibodies to facilitate the study and measurement of this protein in various contexts.
Biological function summary

Thyroglobulin plays a critical role in the synthesis and storage of thyroid hormones. It acts as a scaffold for iodination and hormone synthesis within the colloid of thyroid follicles. Thyroglobulin is not part of a protein complex; rather it undergoes iodination to form hormone precursors that later convert into active hormones. Anti-thyroglobulin antibodies can be used in research or diagnostic labs to detect the presence or concentration of thyroglobulin in serum samples. These antibodies help in understanding the nuances of thyroid hormone biosynthesis and in monitoring thyroid activity.

Pathways

Thyroglobulin is deeply involved in the thyroid hormone synthesis pathway a fundamental part of the endocrine system's operations. This process connects closely with the iodine metabolism pathway as iodine plays an essential role in hormone production. Thyroglobulin interacts indirectly with proteins like thyroid peroxidase which catalyzes iodination of thyroglobulin. The synthesis and release of thyroid hormones proceed through a carefully orchestrated sequence affecting many physiological processes including metabolism growth and development.

Thyroglobulin has associations with conditions such as thyroid cancer and autoimmune thyroid diseases. For instance elevated serum thyroglobulin can serve as a tumor marker in differentiated thyroid cancer. Anti-thyroglobulin antibodies are often present in autoimmune thyroid diseases like Hashimoto's thyroiditis where the body's immune system mistakenly targets thyroid proteins. Thyroid peroxidase antibodies are also common in these disorders indicating a shared autoimmune response against thyroid-specific antigens. Understanding these relationships aids in the diagnosis and management of thyroid-related conditions.
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Product protocols

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Target data

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) (PubMed : 17532758, PubMed : 32025030). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen (PubMed : 32025030). Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream (PubMed : 32025030). One dimer produces 7 thyroid hormone molecules (PubMed : 32025030).
See full target information Thyroglobulin
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 13: PubMed33466826

2021

Prenatal and Postpartum Maternal Iodide Intake from Diet and Supplements, Urinary Iodine and Thyroid Hormone Concentrations in a Region of the United Kingdom with Mild-to-Moderate Iodine Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Diane E Threapleton,Dagmar Waiblinger,Charles J P Snart,Elizabeth Taylor,Claire Keeble,Samina Ashraf,Shazia Bi,Ramzi Ajjan,Rafaq Azad,Neil Hancock,Dan Mason,Stephen Reid,Kirsten J Cromie,Nisreen A Alwan,Michael Zimmermann,Paul M Stewart,Nigel A B Simpson,John Wright,Janet E Cade,Laura J Hardie,Darren C Greenwood

Human cell 33:545-558 PubMed32146707

2020

Thyroid-related hormones as potential markers of hypoxia/ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Naoto Tani,Mayumi Ishikawa,Miho Watanabe,Tomoya Ikeda,Takaki Ishikawa
View all publications
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