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AB178631

Human Anti-Thyroglobulin ELISA Kit

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(1 Publication)

Human Anti-Thyroglobulin ELISA Kit is a Indirect ELISA for the measurement of Human Anti-Thyroglobulin in Human in Biofluids samples.

View Alternative Names

Thyroglobulin, Tg, TG

1 Images
Indirect ELISA - Human Anti-Thyroglobulin ELISA Kit (AB178631)
  • I-ELISA

Supplier Data

Indirect ELISA - Human Anti-Thyroglobulin ELISA Kit (AB178631)

Representative standard curve using ab178631

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Sensitivity

< 0.5 U/mL

Range

2 - 128 U/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Thyroglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to thyroglobulin in Human serum or plasma.

A 96-well plate has been precoated with Human thyroglobulin to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized thyroglobulin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of thyroglobulin sample captured in plate.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178631-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Thyroglobulin Positive Control", "number":"AB178631-CMP12", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroglobulin Negative Control", "number":"AB178631-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB178631-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB178631-CMP03", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroglobulin Standard D - 8 U/mL", "number":"AB178631-CMP16", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB178631-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB178631-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Thyroglobulin Anti-IgG HRP Conjugate", "number":"AB178631-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Thyroglobulin Coated Microplate (12 x 8 wells)", "number":"AB178631-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB178631-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB178631-CMP01", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroglobulin Standard F - 128 U/mL", "number":"AB178631-CMP06", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroglobulin Standard B - 2 U/mL", "number":"AB178631-CMP14", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroglobulin Standard C - 4 U/mL", "number":"AB178631-CMP15", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroglobulin Standard A - 0 U/mL", "number":"AB178631-CMP13", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroglobulin Standard E - 32 U/mL", "number":"AB178631-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anti-Thyroglobulin antibodies target the protein thyroglobulin an important component in thyroid physiology. Known also as TG thyroglobulin is a large glycoprotein with a mass of approximately 660 kDa. It is primarily expressed in the thyroid gland specifically in the follicular cells where it functions as a precursor for thyroid hormones. Thyroglobulin serves as the backbone for the synthesis of thyroxine (T4) and triiodothyronine (T3) essential hormones for metabolic regulation.
Biological function summary

Thyroglobulin connects to thyroid hormone production and is stored within the thyroid follicles as a colloid. It acts as a substrate for the synthesis of T3 and T4 hormones after iodination. Colloid resorption releases thyroid hormones into circulation influencing multiple physiological processes such as growth and metabolism. Thyroglobulin does not operate as a part of a complex; it undergoes enzymatic modification individually to produce active hormones.

Pathways

Thyroglobulin plays a critical role in the thyroid hormone synthesis pathway. Iodine uptake by thyroid cells and coupling with thyroglobulin is central to this process. It interacts closely with proteins like thyroperoxidase which catalyzes iodine addition and the sodium/iodide symporter essential for iodine uptake into cells. The pathway highlights the regulated mechanism that maintains hormone levels for metabolic activities.

Autoantibodies targeting thyroglobulin link to thyroid-related conditions such as Hashimoto's thyroiditis and Graves' disease. These conditions arise when the immune system mistakenly targets thyroglobulin causing dysregulation or destruction of thyroid tissues. Thyroglobulin antibodies often appear alongside anti-thyroperoxidase antibodies suggesting a combined role in triggering immune-related thyroid dysfunctions. Early detection of anti-thyroglobulin antibodies can help diagnose and manage these conditions effectively.
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Product protocols

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Target data

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) (PubMed : 17532758, PubMed : 32025030). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen (PubMed : 32025030). Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream (PubMed : 32025030). One dimer produces 7 thyroid hormone molecules (PubMed : 32025030).
See full target information Thyroglobulin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 10:1875-1881 PubMed26622768

2015

/ mutational status in familial non-medullary thyroid carcinomas: A retrospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Landriscina,Maria Iole Natalicchio,Olga Lamacchia,Antonella Conserva,Annamaria Piscazzi,Anna Ciampolillo,Matteo Zingrillo,Antonio Pennella,Pantaleo Bufo,Giulia Vita,Raffaele Antonetti,Eugenio Maiorano,Francesco Giorgino,Mauro Cignarelli
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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