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AB277455

Human Thyroid Peroxidase/TPO ELISA Kit

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Human Thyroid Peroxidase/TPO ELISA Kit is a Sandwich ELISA for the measurement of Human Thyroid Peroxidase/TPO in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Thyroid peroxidase, TPO

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ELISA - Human Thyroid Peroxidase/TPO ELISA Kit (AB277455)
  • ELISA

Supplier Data

ELISA - Human Thyroid Peroxidase/TPO ELISA Kit (AB277455)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.3 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB278525

Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (AB278525)

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

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Human ErbB3 ELISA Kit (HER3)

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Sandwich ELISA - Human ErbB3 ELISA Kit (HER3) (AB275101)

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Primary Antibodies

AB108421

Anti-DcR2 antibody [EPR3588(2)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (AB108421)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Thyroid Peroxidase/TPO ELISA Kit (ab277455) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thyroid Peroxidase/TPO in serum, plasma (Collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay), and cell culture supernatants.

This assay employs an antibody specific for Human Thyroid Peroxidase/TPO coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Thyroid Peroxidase/TPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Thyroid Peroxidase/TPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Thyroid Peroxidase/TPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "72 - 144 %", "average": "= 107.4" }, { "sample": "Cell culture media", "range": "123 - 148 %", "average": "= 136.7" }, { "sample": "Plasma", "range": "122 - 148 %", "average": "= 135.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277455-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277455-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277455-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Thyroid Peroxidase TPO coated Microplate", "number":"AB277455-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277455-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277455-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277455-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277455-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Thyroid Peroxidase/TPO Antibody", "number":"AB277455-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Thyroid Peroxidase/TPO standard protein (Lyophilized)", "number":"AB277455-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thyroid Peroxidase (TPO) also known as thyroperoxidase is an enzyme with a molecular mass of around 105 kDa. It is expressed in the thyroid gland's follicular cells. TPO plays a mechanical role in the synthesis of thyroid hormones by catalyzing iodination and coupling reactions needed to produce triiodothyronine (T3) and thyroxine (T4). These hormones are essential for regulating metabolism growth and development. The activity of TPO is an important step in thyroid hormone production.
Biological function summary

TPO participates in the thyroid's hormone biosynthesis pathway. It forms a part of the multi-enzyme complex that includes thyroglobulin and iodide. The enzyme facilitates the iodination of tyrosine residues on thyroglobulin and the coupling of iodotyrosines important for creating active thyroid hormones. TPO's enzymatic function is important for maintaining appropriate levels of circulating T3 and T4 impacting various physiological processes in the body.

Pathways

TPO contributes to the thyroid hormone synthesis pathway which is a critical pathway for metabolic regulation. It works in close association with other proteins such as thyroglobulin and sodium/iodide symporter (NIS) to ensure efficient thyroid hormone production. TPO's involvement in these pathways indicates its importance in the endocrine system's normal function and homeostasis.

TPO is often associated with autoimmune thyroid diseases like Hashimoto's thyroiditis and Graves' disease. High TPO antibodies such as anti-TPO antibodies are commonly detected in these conditions indicating an autoimmune response against the thyroid. The presence of high anti-thyroid peroxidase antibodies can lead to the destruction of thyroid tissue often resulting in hypothyroidism. TPO's connection to these diseases highlights its role in autoimmune conditions affecting thyroid function.
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Product protocols

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Target data

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
See full target information Thyroid peroxidase
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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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