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AB100660

Human Thyroid Stimulating Hormone ELISA Kit (TSH)

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(1 Publication)

Human Thyroid Stimulating Hormone ELISA Kit (TSH) is a Sandwich (quantitative) ELISA for the measurement of Human Thyroid Stimulating Hormone (TSH) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha, CGA

2 Images
Sandwich ELISA - Human Thyroid Stimulating Hormone ELISA Kit (TSH) (AB100660)
  • sELISA

Unknown

Sandwich ELISA - Human Thyroid Stimulating Hormone ELISA Kit (TSH) (AB100660)

Representative standard curve using ab100660

Sandwich ELISA - Human Thyroid Stimulating Hormone ELISA Kit (TSH) (AB100660)
  • sELISA

Unknown

Sandwich ELISA - Human Thyroid Stimulating Hormone ELISA Kit (TSH) (AB100660)

Representative standard curve using ab100660

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 4 pg/mL

Range

2.744 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TSH Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TSH in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human TSH coated on a 96-well plate. Standards and samples are pipetted into the wells and TSH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSH antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "70 - 90 %", "average": "= 82.76" }, { "sample": "Serum", "range": "75 - 120 %", "average": "= 97.89" }, { "sample": "Plasma", "range": "67 - 86 %", "average": "= 78.59" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100660-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100660-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100660-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TSH Microplate (12 x 8 wells)", "number":"AB100660-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100660-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human TSH Standard (lyophilized)", "number":"AB100660-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human TSH", "number":"AB100660-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100660-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100660-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100660-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSH also known as thyrotropin or thyroid-stimulating hormone is a glycoprotein hormone with a molecular mass around 28 kDa. It is produced and secreted by the anterior pituitary gland. TSH plays a central role in the endocrine system by stimulating the thyroid gland to release thyroid hormones. These hormones are important for maintaining the body's metabolism growth and development.
Biological function summary

Thyroid-stimulating hormone acts by binding to receptors on the surface of thyroid follicular cells. This binding stimulates synthesis and secretion of thyroxine (T4) and triiodothyronine (T3). TSH is part of the hypothalamic-pituitary-thyroid axis a feedback loop that involves the hypothalamus and pituitary gland to regulate thyroid hormone levels. This axis is vital for maintaining metabolic stability in the body.

Pathways

Thyroid-stimulating hormone plays a significant role in the hypothalamic-pituitary-thyroid (HPT) axis. This pathway regulates the synthesis and release of thyroid hormones involving three main components: thyrotropin-releasing hormone (TRH) from the hypothalamus TSH from the pituitary and thyroid hormones from the thyroid gland. These components work together to ensure proper metabolic homeostasis. Additionally TSH impacts the JAK-STAT signaling pathway influencing cell proliferation and immunity response.

Thyroid-stimulating hormone is closely associated with conditions like Hashimoto's thyroiditis and Graves' disease. In Hashimoto's an autoimmune disorder antibodies attack the thyroid often leading to elevated TSH levels as the body attempts to stimulate the underactive thyroid. In contrast Graves' disease shows reduced TSH levels due to the thyroid being overactive. TSH measurement helps in diagnosing these conditions and understanding the dysregulation of thyroid hormones in these patients.
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Product protocols

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Target data

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.
See full target information CGA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 9:945622 PubMed35903454

2022

A Pilot Pre and Post 4 Week Intervention Evaluating the Effect of a Proprietary, Powdered, Plant Based Food on Micronutrient Status, Dietary Intake, and Markers of Health in a Healthy Adult Population.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Wilcox,Peter I Chater,Kyle J Stanforth,Rebecca Williams,Iain A Brownlee,Jeffrey P Pearson
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websiteProtocolBooklet
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