Human Thyroxine Binding Globulin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Thyroxine Binding Globulin in Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
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- Heliyon 9:e216162023Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingyan Chen,Jingyang Li,Yilan You,Zhiwen Qian,Jiayu Liu,Ying Jiang,Ying Gu,Jianping Xiao,Yan ZhangPubMed 38027820
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
18.75 - 1200 pg/mL
- Assay time
1h 30m
- Sensitivity
= 8.624 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Major thyroid hormone transport protein in serum.
Alternative names
TBG, TBG, SERPINA7, Thyroxine-binding globulin, Serpin A7, T4-binding globulin
What's included?
Recommended products
Human Thyroxine Binding Globulin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Thyroxine Binding Globulin in Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
18.75 - 1200 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 8.624 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 7.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 11.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 94 | Range 81 - 113 % |
Sample type EDTA Plasma | Average % = 109 | Range 107 - 113 % |
Sample type Cell culture media | Average % = 115 | Range 112 - 117 % |
Sample type Heparin Plasma | Average % = 108 | Range 93 - 116 % |
Sample type Citrate plasma | Average % = 91 | Range 87 - 96 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Thyroxine Binding Globulin ELISA Kit (ab289642) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Thyroxine Binding Globulin in serum, plasma (heparin), plasma (EDTA), plasma (citrate), and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thyroxine Binding Globulin with 8.624 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thyroxine Binding Globulin (TBG) also called globulin 23 is a glycoprotein that binds thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3). It facilitates the transport of these hormones through the bloodstream to various tissues. This binding globulin has a molecular weight of approximately 54 kDa. TBG is predominantly expressed in the liver and is an essential component within the circulatory system for hormone regulation. Although there is no substance called 'globulin green' TBG plays an important role in maintaining the systemic balance of thyroid hormones.
- Biological function summary
The thyroxine-binding globulin helps maintain adequate levels of thyroid hormones in circulation ensuring a ready reserve supply to target tissues. TBG acts as a carrier protein and does not form part of a larger complex. It provides stability to the hormones and prevents their premature clearance from the bloodstream. Without it the availability and functionality of thyroid hormones within the body would be compromised affecting metabolic and growth functions.
- Pathways
Thyroxine-binding globulin is critical in the thyroid hormone signaling pathway. This pathway regulates metabolism growth and development through the action of thyroid hormones. TBG is closely related to other hormone-binding proteins such as transthyretin and albumin which also participate in thyroid hormone transport albeit with different affinities. Its presence ensures that these hormones remain available for cellular uptake and action within various tissues influencing energy expenditure and developmental processes.
- Associated diseases and disorders
The function and levels of thyroxine-binding globulin have significant implications in conditions like hypothyroidism and hyperthyroidism. Altered TBG levels either due to genetic variations or external factors can lead to abnormal blood concentrations of thyroid hormones affecting growth and metabolism. In such disorders the interaction between TBG and thyroid hormones becomes critical. TBG levels can be influenced by other proteins like sex hormone-binding globulin (SHBG) which may reflect changes in the hormone-binding protein landscape during disease states contributing to dysregulated hormonal balance.
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3 product images
Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (ab289642)
Interpolated concentrations of human Thyroxine Binding Globulin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).
Interpolated concentration of native Thyroxine Binding Globulin was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:100, plasma (EDTA) 1:100, and plasma (heparin) 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (ab289642)
Interpolated concentrations of spiked human Thyroxine Binding Globulin in cell culture media (10F RPMI).
Recombinant Thyroxine Binding Globulin was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% cell culture media (10F RPMI). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (ab289642)
Example of human Thyroxine Binding Globulin standard curve in Sample Diluent NS.
Example of human Thyroxine Binding Globulin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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