AB289642

Human Thyroxine Binding Globulin ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Human Thyroxine Binding Globulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thyroxine Binding Globulin with a sensitivity of 8.624 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TBG, SERPINA7, Thyroxine-binding globulin, Serpin A7, T4-binding globulin

3 Images

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

sELISA

Supplier Data

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Interpolated concentrations of human Thyroxine Binding Globulin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native Thyroxine Binding Globulin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 200, plasma (citrate) 1 : 100, plasma (EDTA) 1 : 100, and plasma (heparin) 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Interpolated concentrations of spiked human Thyroxine Binding Globulin in cell culture media (10F RPMI).

Recombinant Thyroxine Binding Globulin was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% cell culture media (10F RPMI). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Example of human Thyroxine Binding Globulin standard curve in Sample Diluent NS.

Example of human Thyroxine Binding Globulin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8.624 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human Thyroxine Binding Globulin ELISA Kit (ab289642) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Thyroxine Binding Globulin in serum, plasma (heparin), plasma (EDTA), plasma (citrate), and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thyroxine Binding Globulin with 8.624 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "81 - 113 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "107 - 113 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "112 - 117 %", "average": "= 115" }, { "sample": "Heparin Plasma", "range": "93 - 116 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "87 - 96 %", "average": "= 91" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289642-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289642-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289642-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289642-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289642-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289642-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Thyroxine Binding Globulin Capture Antibody", "number":"AB289642-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Thyroxine Binding Globulin Lyophilized Recombinant Protein", "number":"AB289642-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB289642-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Thyroxine Binding Globulin Detector Antibody", "number":"AB289642-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289642-CMP04", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thyroxine Binding Globulin (TBG) also called globulin 23 is a glycoprotein that binds thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3). It facilitates the transport of these hormones through the bloodstream to various tissues. This binding globulin has a molecular weight of approximately 54 kDa. TBG is predominantly expressed in the liver and is an essential component within the circulatory system for hormone regulation. Although there is no substance called 'globulin green' TBG plays an important role in maintaining the systemic balance of thyroid hormones.

Biological function summary

The thyroxine-binding globulin helps maintain adequate levels of thyroid hormones in circulation ensuring a ready reserve supply to target tissues. TBG acts as a carrier protein and does not form part of a larger complex. It provides stability to the hormones and prevents their premature clearance from the bloodstream. Without it the availability and functionality of thyroid hormones within the body would be compromised affecting metabolic and growth functions.

Pathways

Thyroxine-binding globulin is critical in the thyroid hormone signaling pathway. This pathway regulates metabolism growth and development through the action of thyroid hormones. TBG is closely related to other hormone-binding proteins such as transthyretin and albumin which also participate in thyroid hormone transport albeit with different affinities. Its presence ensures that these hormones remain available for cellular uptake and action within various tissues influencing energy expenditure and developmental processes.

The function and levels of thyroxine-binding globulin have significant implications in conditions like hypothyroidism and hyperthyroidism. Altered TBG levels either due to genetic variations or external factors can lead to abnormal blood concentrations of thyroid hormones affecting growth and metabolism. In such disorders the interaction between TBG and thyroid hormones becomes critical. TBG levels can be influenced by other proteins like sex hormone-binding globulin (SHBG) which may reflect changes in the hormone-binding protein landscape during disease states contributing to dysregulated hormonal balance.

style

left-column

Product protocols

style

left-column

Target data

Major thyroid hormone transport protein in serum.

See full target information SERPINA7

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e21616 PubMed38027820

2023

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Chen,Jingyang Li,Yilan You,Zhiwen Qian,Jiayu Liu,Ying Jiang,Ying Gu,Jianping Xiao,Yan Zhang

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Thyroxine Binding Globulin ELISA Kit

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Product promise