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AB108763

Human Tick-Borne Encephalitis Virus plus IgG ELISA Kit (TBE/FSME)

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Human Tick-Borne Encephalitis Virus plus IgG ELISA Kit (TBE/FSME) is an indirect ELISA for the qualitative detection of IgG class antibodies against Tick-Borne Encephalitis Virus plus (TBE/FSME) in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Tick-borne encephalitis (TBE) virus IgG plus Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Tick-borne encephalitis virus in Human serum and plasma.

A 96-well plate has been precoated with Tick-borne encephalitis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Tick-borne encephalitis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tick-borne encephalitis IgG sample captured in plate.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Standard E", "replicates": 8, "mean": "2.15 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 4.2" }, { "reproducibilityType": "Inter", "sample": "Standard C", "replicates": 16, "mean": "0.92 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 8.3" }, { "reproducibilityType": "Intra", "sample": "Standard C", "replicates": 8, "mean": "0.98 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 5.7" }, { "reproducibilityType": "Intra", "sample": "Standard B", "replicates": 7, "mean": "0.32 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 6.8" }, { "reproducibilityType": "Inter", "sample": "Standard D", "replicates": 15, "mean": "1.37 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 7.8" }, { "reproducibilityType": "Inter", "sample": "Standard B", "replicates": 14, "mean": "0.31 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 7.35" }, { "reproducibilityType": "Inter", "sample": "Standard E", "replicates": 16, "mean": "2.28 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 6.7" }, { "reproducibilityType": "Intra", "sample": "Standard D", "replicates": 8, "mean": "1.3 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 4.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108763-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"TBE virus IgG Standard C – 130 U/mL", "number":"AB108763-CMP09", "productcode":"" }, { "size":"1 x 2 mL", "name":"TBE virus IgG Standard E – 300 U/mL", "number":"AB108763-CMP06", "productcode":"" }, { "size":"1 x 2 mL", "name":"TBE virus IgG Standard A – 0 U/mL", "number":"AB108763-CMP07", "productcode":"" }, { "size":"1 x 2 mL", "name":"TBE virus IgG Additional Control", "number":"AB108763-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"TBE virus IgG Standard B – 50 U/mL", "number":"AB108763-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108763-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TBE virus (IgG) Coated Microplate (12 x 8 wells)", "number":"AB108763-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108763-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"TBE virus anti-IgG HRP Conjugate", "number":"AB108763-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108763-CMP13", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108763-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108763-CMP14", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108763-CMP12", "productcode":"" }, { "size":"1 x 2 mL", "name":"TBE virus IgG Standard D – 200 U/mL", "number":"AB108763-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Tick-Borne Encephalitis Virus also known as FSME virus circulates in certain geographic regions and causes infections in humans. This virus comprises a single positive-strand RNA inside an icosahedral capsid and measures approximately 45-50nm in diameter. It primarily expresses in infected host cells facilitating viral assembly and replication. The structure of the virus allows it to attach to host cell receptors aiding its entry and invasion into the central nervous system.
Biological function summary

The Tick-Borne Encephalitis virus interacts with the immune system through the production of specific antibodies including IgG. These antibodies form part of the host's immune response targeting viral antigens to neutralize infection. The virus itself however does not belong to a larger protein complex but its structural proteins enable interaction with host immune cells sparking antibody production detected using ELISA tests.

Pathways

Viral entry and replication of the Tick-Borne Encephalitis virus engage with signal transduction and immune response pathways. The interferon signaling pathway becomes activated when the virus enters the host facilitating an antiviral state within infected cells. In this context the virus interacts with proteins such as IFN-alpha and IFN-beta influencing the host's antiviral defenses.

The Tick-Borne Encephalitis virus associates closely with encephalitis a severe inflammatory condition of the brain. The presence and activity of the virus can lead to CNS disorders resulting in neurological symptoms in affected individuals. Sero-diagnostic ELISA tests frequently evaluate the presence of TBE-specific IgG antibodies in patient serum providing insights into viral activity and subsequent antibody-mediated immune response in related infections.
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Product protocols

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Target data

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websiteProtocolBooklet
en
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