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AB100649

Human TIE1 ELISA Kit

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Human TIE1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TIE1 in Human in Cell Culture Media samples.

View Alternative Names

TIE, TIE1, Tyrosine-protein kinase receptor Tie-1

2 Images
Sandwich ELISA - Human TIE1 ELISA Kit (AB100649)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIE1 ELISA Kit (AB100649)

Representative standard curve using ab100649

Sandwich ELISA - Human TIE1 ELISA Kit (AB100649)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIE1 ELISA Kit (AB100649)

Representative standard curve using ab100649

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.6 ng/mL

Range

0.68 - 500 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TIE1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIE1 in cell culture supernatants.

This assay employs an antibody specific for Human TIE1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIE1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human Tie-1 in normal serum with ab100649, only in serum spiked with Human Tie-1. Plasma is not recommended.

Serum has been validated as a sample matrix with this kit. However, TIE1 concentrations are low in normal serum and therefore may not be detected by this assay.

Plasma is not recommended.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 115 %", "average": "= 105.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100649-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100649-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100649-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human TIE1", "number":"AB100649-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100649-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100649-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TIE1 Microplate (12 x 8 wells)", "number":"AB100649-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100649-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100649-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human TIE1 Standard (lyophilized)", "number":"AB100649-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIE1 also known as Tyrosine Kinase with Immunoglobulin-like and EGF-like Domains 1 plays a role as a receptor tyrosine kinase. It has a molecular mass of approximately 130 kDa. TIE1 is mainly expressed in vascular endothelial cells and is involved in the vascular system. It shares structural characteristics with other tyrosine kinases providing a framework for signaling and interaction. TIE1 activation involves phosphorylation leading to subsequent signaling cascades in endothelial cells.
Biological function summary

TIE1 associates with angiogenesis and blood vessel integrity. It forms a complex with other proteins notably TIE2 within the endothelial cells where it modulates endothelial cell survival and proliferation. Its role becomes particularly significant in dynamic environments where new blood vessel formation or remodeling occurs. TIE1 interacts with angiopoietins providing a network essential for maintaining vascular homeostasis and integrity.

Pathways

TIE1 interacts within angiopoietin-TIE signaling pathways. This pathway plays a critical role in vascular maturation and stability. TIE1 working in conjunction with TIE2 and the angiopoietins orchestrates the intricate balance between vessel stabilization and sprouting needed for healthy vascular function. These interactions link TIE1 with core processes in endothelial cell communication and vascular development.

TIE1 shows association with cancer and atherosclerosis. Its interaction with TIE2 and angiopoietin proteins contributes to pathological angiogenesis often observed in tumor growth and progression. In atherosclerosis TIE1 influences endothelial dysfunction and vascular inflammation. These diseases highlight TIE1's contribution to pathological processes highlighting the importance of its precise regulation in maintaining vascular health.
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Product protocols

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Target data

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
See full target information TIE1
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websiteProtocolBooklet
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