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AB314705

Human TIL/TLR1 ELISA Kit

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Human TIL/TLR1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TIL/TLR1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CD281, KIAA0012, TLR1, Toll-like receptor 1, Toll/interleukin-1 receptor-like protein, TIL

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Sandwich ELISA - Human TIL/TLR1 ELISA Kit (AB314705)
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Supplier Data

Sandwich ELISA - Human TIL/TLR1 ELISA Kit (AB314705)

Example of standard curve using ab314705. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 16 pg/mL

Range

16 - 4000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TIL/TLR1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TIL/TLR1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human TIL/TLR1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "91 - 107 %", "average": "= 110.5" }, { "sample": "Plasma", "range": "93 - 129 %", "average": "= 109.6" }, { "sample": "Cell culture media", "range": "101 - 122 %", "average": "= 113.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314705-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314705-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human TIL/TLR1 Antibody-coated ELISA Plate", "number":"AB314705-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314705-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314705-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human TIL/TLR1 Detection Antibody", "number":"AB314705-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314705-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human TIL/TLR1 Protein Standard", "number":"AB314705-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314705-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toll-like receptor 1 (TLR1) also known as TIL plays a significant role in the immune system. It is a pattern recognition receptor that detects pathogen-associated molecular patterns (PAMPs). TLR1 has a molecular weight of approximately 90 kilodaltons. It is expressed on the surface of various immune cells including monocytes macrophages and dendritic cells. TLR1 recognizes triacylated lipopeptides in combination with TLR2 forming a heterodimer that is essential for signaling. This interaction enhances the immune response against microbial infections.
Biological function summary

TLR1 contributes to the immune response by participating in the detection of bacterial lipoproteins. This receptor is a part of the Toll-like receptor family which plays an essential role in innate immunity. TLR1 often in complex with TLR2 helps in recognizing and responding to the structural motifs of microbial pathogens. These interactions lead to the activation of downstream signaling pathways triggering the production of cytokines and other mediators essential for inflammatory responses.

Pathways

TLR1 is involved in the Toll-like receptor signaling pathway and the NF-kB signaling pathway. In the Toll-like receptor pathway TLR1-TLR2 heterodimers signal through adaptor proteins like MyD88 which leads to the activation of transcription factors such as NF-kB. This mechanism regulates the expression of genes involved in immune and inflammatory responses. TLR1 coordinates with TLR2 not just in recognition of ligands but also in the signaling to produce a coordinated immune response enhancing the cell's ability to deal with infections.

TLR1 plays a role in conditions involving immune dysregulation such as tuberculosis and sepsis. Genetic variations in TLR1 can impact susceptibility to these diseases by altering the receptor's ability to recognize pathogenic components. TLR1's interaction with TLR2 influences the inflammatory response and may contribute to inappropriate immune activation seen in sepsis. These receptors' roles in inflammation and pathogen recognition highlight their importance in understanding the development and progression of immune-related diseases.
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Product protocols

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Target data

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed : 21078852). Forms the activation cluster TLR2 : TLR1 : CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
See full target information TLR1
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Complementary Products

Primary Antibodies

AB68158

Anti-TIL/TLR1 antibody [EPR2075]

RabMAb|Recombinant

Western blot - Anti-TIL/TLR1 antibody [EPR2075] (AB68158)

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