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AB289703

Human TIM 1 ELISA Kit

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Human TIM 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TIM 1 with a sensitivity of 48.877 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD365, KIM1, TIM1, TIMD1, HAVCR1, Hepatitis A virus cellular receptor 1, HAVcr-1, Kidney injury molecule 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell immunoglobulin mucin receptor 1, T-cell membrane protein 1, KIM-1, TIMD-1, TIM, TIM-1

3 Images
Sandwich ELISA - Human TIM 1 ELISA Kit (AB289703)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIM 1 ELISA Kit (AB289703)

Human TIM 1 competitor standard curve comparison.

Standard curve comparison between Human TIM 1 SimpleStep ELISA kit (ab289703) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity

Sandwich ELISA - Human TIM 1 ELISA Kit (AB289703)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIM 1 ELISA Kit (AB289703)

Interpolated concentrations of Human TIM 1 in Urine and JEG-3 cell culture supernatant.

Interpolated concentration of native TIM 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Urine 50% and JEG-3 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TIM 1 ELISA Kit (AB289703)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIM 1 ELISA Kit (AB289703)

Example of Human TIM 1 standard curve in Sample Diluent NS.

Example of Human TIM 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 48.877 pg/mL

Range

218.75 - 14000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

<p>Human TIM 1 ELISA Kit (ab289703) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TIM 1 in urine and cell culture supernatants samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate TIM 1 with 48.877 pg/ml sensitivity.</p>

<p>SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:</p>

<p>Single-wash protocol reduces assay time to 90 minutes or less</p><p>High sensitivity, specificity and reproducibility from superior antibodies</p>

<p>Fully validated in biological samples</p><p>96-wells plate breakable into 12 x 8 wells strips</p>

<p>A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.</p>

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "105 - 112 %", "average": "= 109" }, { "sample": "Urine", "range": "96 - 108 %", "average": "= 101" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289703-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289703-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289703-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289703-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289703-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289703-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TIM 1 Lyophilized Recombinant Protein", "number":"AB289703-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TIM 1 Capture Antibody", "number":"AB289703-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB289703-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TIM 1 Detector Antibody", "number":"AB289703-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289703-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.
Biological function summary

The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein’s activity influences cytokine production and this can affect inflammation processes.

Pathways

TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.

TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1’s involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.
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Product protocols

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Target data

Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions (By similarity). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses (PubMed : 24703780). Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (PubMed : 24703780). Regulates also expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10 (By similarity). Acts as a regulator of T-cell proliferation (By similarity). May play a role in kidney injury and repair (PubMed : 17471468).. (Microbial infection) Acts as a receptor for Hepatitis A virus.. (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed : 21536871). Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP (PubMed : 26487564).. (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed : 23084921). TIM1 and Dengue virus are co-internalized during virus entry (PubMed : 29742433).. (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E.. (Microbial infection) Plays a positive role in Chikungunya virus cell entry.
See full target information HAVCR1
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