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AB100651

Human TIMP1 ELISA Kit

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(6 Publications)

Human TIMP1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TIMP1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1

2 Images
Sandwich ELISA - Human TIMP1 ELISA Kit (AB100651)
  • sELISA

Unknown

Sandwich ELISA - Human TIMP1 ELISA Kit (AB100651)

Representative standard curve using ab100651

Sandwich ELISA - Human TIMP1 ELISA Kit (AB100651)
  • sELISA

Unknown

Sandwich ELISA - Human TIMP1 ELISA Kit (AB100651)

Representative standard curve using ab100651

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 40 pg/mL

Range

24.69 - 18000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TIMP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Human TIMP1 ELISA Kit (ab187394) from our SimpleStep ELISA® range.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 94.49" }, { "sample": "Serum", "range": "81 - 102 %", "average": "= 92.43" }, { "sample": "Plasma", "range": "83 - 102 %", "average": "= 93.67" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100651-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100651-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100651-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100651-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TIMP1 Microplate (12 x 8 wells)", "number":"AB100651-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100651-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human TIMP1 Standard (lyophilized)", "number":"AB100651-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100651-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human TIMP1", "number":"AB100651-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100651-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
Biological function summary

TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.

Pathways

TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.

TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
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Product protocols

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Target data

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
See full target information TIMP1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 17:2433-2442 PubMed30906430

2019

Impact of several proinflammatory and cell degradation factors in patients with aortic valve stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Juris Lurins,Dace Lurina,Simons Svirskis,Zaiga Nora-Krukle,Peteris Tretjakovs,Vitolds Mackevics,Aivars Lejnieks,Venerando Rapisarda,Vincenzo Baylon

PloS one 13:e0191793 PubMed29385192

2018

Anti-influenza A virus activity of rhein through regulating oxidative stress, TLR4, Akt, MAPK, and NF-κB signal pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qian-Wen Wang,Yun Su,Jiang-Tao Sheng,Li-Ming Gu,Ying Zhao,Xiao-Xuan Chen,Cheng Chen,Wei-Zhong Li,Kang-Sheng Li,Jian-Ping Dai

Contemporary clinical trials communications 8:33-38 PubMed29696194

2017

Rationale and design of DUAL study: Doxycycline to Upgrade response in light chain (AL) amyloidosis (DUAL): A phase 2 pilot study of a two-pronged approach of prolonged doxycycline with plasma cell-directed therapy in the treatment of AL amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Anita D'Souza,Kathryn Flynn,Saurabh Chhabra,Binod Dhakal,Mehdi Hamadani,Kirsten Jacobsen,Marcelo Pasquini,Dorothee Weihrauch,Parameswaran Hari

Global cardiology science & practice 2015:49 PubMed26779524

2015

Serum and tissue biomarkers in aortic stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alkistis Kapelouzou,Loukas Tsourelis,Loukas Kaklamanis,Dimitrios Degiannis,Nektarios Kogerakis,Dennis V Cokkinos

Annals of clinical and translational neurology 2:1071-84 PubMed26734659

2015

Pioglitazone regulates myelin phagocytosis and multiple sclerosis monocytes.

Applications

ELISA

Species

Human

Muktha S Natrajan,Mika Komori,Peter Kosa,Kory R Johnson,Tianxia Wu,Robin J M Franklin,Bibiana Bielekova

Reproductive biology and endocrinology : RB&E 13:102 PubMed26337061

2015

Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Jui Yang,Fon-Chang Liu,Jih-Sheng Hsieh,Ching-Hung Chen,Shun-Yu Hsiao,Chih-Sheng Lin
View all publications
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websiteProtocolBooklet
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