Human TIMP1 ELISA Kit ab187394 is a single-wash 90-min SimpleStep ELISA used to quantify Human TIMP1 with a sensitivity of 17 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human TIMP1 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
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- Scientific reports 13:179692023The combination of cigarette smoke and solar rays causes effects similar to skin aging in a bilayer skin model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexe Grenier,Mathieu C Morissette,Patrick J Rochette,Roxane PouliotPubMed 37863977
- International journal of molecular sciences 23:2022Gene Expression Linked to Reepithelialization of Human Skin Wounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagnus S Ågren,Thomas Litman,Jens Ole Eriksen,Peter Schjerling,Michael Bzorek,Lise Mette Rahbek GjerdrumPubMed 36555389
- Acta dermato-venereologica 102:adv007712022Corrigendum: Biomarkers of Skin Graft Healing in Venous Leg Ulcers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKlaus Kirketerp-Møller,Petra Doerfler,Nicole Schoefmann,Barbara Wolff-Winiski,Omid Niazi,Vibeke Pless,Tonny Karlsmark,Magnus S ÅgrenPubMed 36039412
- Acta dermato-venereologica 102:adv007492022Biomarkers of Skin Graft Healing in Venous Leg Ulcers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKlaus Kirketerp-Møller,Petra Doerfler,Nicole Schoefmann,Barbara Wolff-Winiski,Omid Niazi,Vibeke Pless,Tonny Karlsmark,Magnus S. ÅgrenPubMed 35604238
- Journal of oncology 2022:83192212022Effects of Surgery Combined with Different Chemotherapy on Matrix Metalloproteinase-9 and Tissue Inhibitors of Metalloproteinase-1 in Children with Neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZilong Qi,Xinning Wang,Liang Yin,Kun Ma,Lei Chen,Jinmin LiPubMed 35847358
- Hepatology (Baltimore, Md.) 77:558-5722022Circulating TREM2 as a noninvasive diagnostic biomarker for NASH in patients with elevated liver stiffness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVineesh Indira Chandran,Charlotte Wilhelmina Wernberg,Mette Munk Lauridsen,Maria Kløjgaard Skytthe,Sofie Marchsteiner Bendixen,Frederik Tibert Larsen,Camilla Dalby Hansen,Lea Ladegaard Grønkjær,Majken Storm Siersbæk,Tina Di Caterino,Sönke Detlefsen,Holger Jon Møller,Lars Grøntved,Kim Ravnskjaer,Søren Kragh Moestrup,Maja Sofie Thiele,Aleksander Krag,Jonas Heilskov GraversenPubMed 35712786
- Veterinary ophthalmology 24:279-2872021Determining MMP-2 and MMP-9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErotides Capistrano da Silva,Daniel J Gibson,Sunyoung Jeong,Kelli L Zimmerman,Kathryn M Smith-Fleming,Bianca da C MartinsPubMed 33834598
- BMC cardiovascular disorders 20:4682020Atrial matrix remodeling in atrial fibrillation patients with aortic stenosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Fragão-Marques et. al.PubMed 33129260
- Cellular signalling 72:1096612020Fibrin fragment E potentiates TGF-β-induced myofibroblast activation and recruitment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeder Öhman Fuchs et. al.PubMed 32334027
- Cardiovascular research 105:304-172015Cardiomyocyte-specific expression of CYP2J2 prevents development of cardiac remodelling induced by angiotensin II.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZuowen He et. al.PubMed 25618409
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 17 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
Alternative names
CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1
What's included?
Recommended products
Human TIMP1 ELISA Kit ab187394 is a single-wash 90-min SimpleStep ELISA used to quantify Human TIMP1 with a sensitivity of 17 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human TIMP1 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 17 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 0.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 89 | Range 86 - 92 % |
Sample type Serum | Average % = 89 | Range 89 - 90 % |
Sample type EDTA Plasma | Average % = 89 | Range 86 - 91 % |
Sample type Saliva | Average % = 84 | Range 82 - 87 % |
Sample type Heparin Plasma | Average % = 95 | Range 94 - 98 % |
Sample type Citrate plasma | Average % = 97 | Range 95 - 99 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human TIMP1 ELISA Kit (ab187394) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIMP1 protein in cit plasma, edta plasma, hep plasma, serum, saliva, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TIMP1 with 17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Tissue inhibitor of metalloproteinases 1 (TIMP1 or Metalloproteinase inhibitor 1) is a widely expressed inhibitor of matrix metalloproteinases (MMPs). It functions by binding non-covalently to MMPs (in a 1:1 stoichiometry) and blocking access of substrate to the MMP active site. TIMP1 expression is induced by pro-inflammatory cytokines. It is a soluble factor found circulating in serum and plasma. TIMP1 appears also have functions that are independent of MMP inhibition, including promoting erythropoiesis and inhibiting apoptosis in B cells.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
- Biological function summary
TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.
- Pathways
TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.
- Associated diseases and disorders
TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
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7 product images
Sandwich ELISA - Human TIMP1 ELISA Kit (ab187394)
Human TIMP-1 competitor curve comparison.
Standard Curve comparison between Human TIMP-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human TIMP1 ELISA Kit (ab187394)
Human TIMP-1 standard curve comparison.
Standard Curve comparison between the original Human TIMP-1 SimpleStep ELISA kit and current Human TIMP-1 SimpleStep ELISA kit.
Sandwich ELISA - Human TIMP1 ELISA Kit (ab187394)
Example of human TIMP-1 standard curve in Sample Diluent NS.
The TIMP-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human TIMP1 ELISA Kit (ab187394)
Interpolated concentrations of native TIMP-1 in human serum, and plasma samples.
The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 95.9 ng/mL in serum, 74 ng/mL in plasma (citrate), 68 ng/mL in plasma (EDTA) and 56 ng/mL in plasma (heparin).
Sandwich ELISA - Human TIMP1 ELISA Kit (ab187394)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 92.6 ng/mL with a range of 66.5 – 128.7 ng/mL.
Sandwich ELISA - Human TIMP1 ELISA Kit (ab187394)
Interpolated concentrations of native TIMP-1 in human cell culture supernatant samples.
The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC cell culture supernatant 20% and stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 5 ng/mL in unstimulated and 22 ng/mL in stimulated and undetectable in media.
Sandwich ELISA - Human TIMP1 ELISA Kit (ab187394)
Interpolated concentrations of native TIMP-1 in human saliva sample.
The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 2%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 179.4 ng/mL in saliva.
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