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AB187394

Human TIMP1 ELISA Kit

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(11 Publications)

Human TIMP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TIMP1 with a sensitivity of 17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1

10 Images
ELISA - Human TIMP1 ELISA Kit (AB187394)
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Supplier Data

ELISA - Human TIMP1 ELISA Kit (AB187394)
Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
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Lab

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Example of human TIMP-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Lab

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Interpolated concentration of native TIMP-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Human TIMP-1 standard curve comparison.

Standard Curve comparison between the original Human TIMP-1 SimpleStep ELISA kit and current Human TIMP-1 SimpleStep ELISA kit.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Interpolated concentrations of native TIMP-1 in human cell culture supernatant samples.

The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated PBMC cell culture supernatant 20% and stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 5 ng/mL in unstimulated and 22 ng/mL in stimulated and undetectable in media.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Interpolated concentrations of native TIMP-1 in human serum, and plasma samples.

The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 95.9 ng/mL in serum, 74 ng/mL in plasma (citrate), 68 ng/mL in plasma (EDTA) and 56 ng/mL in plasma (heparin).

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Human TIMP-1 competitor curve comparison.

Standard Curve comparison between Human TIMP-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Example of human TIMP-1 standard curve in Sample Diluent NS.

The TIMP-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Interpolated concentrations of native TIMP-1 in human saliva sample.

The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 2%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 179.4 ng/mL in saliva.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 92.6 ng/mL with a range of 66.5 – 128.7 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 17 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TIMP1 ELISA Kit ab187394 is a rapid single-wash 90-min Sandwich ELISA to measure Human TIMP1 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum. This SimpleStep sensitivity is 17 pg/mL.

How the assay works

Human TIMP1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TIMP1 ELISA Kit ab187394 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human TIMP1 ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TIMP1 antibody [EPR21887-129] - BSA and Azide free (Capture) ab244890
- Anti-TIMP1 antibody [EPR21887-34] - BSA and Azide free (Detector) ab244890

Tissue inhibitor of metalloproteinases 1 (TIMP1 or Metalloproteinase inhibitor 1) is a widely expressed inhibitor of matrix metalloproteinases (MMPs). It functions by binding non-covalently to MMPs (in a 1:1 stoichiometry) and blocking access of substrate to the MMP active site. TIMP1 expression is induced by pro-inflammatory cytokines. It is a soluble factor found circulating in serum and plasma. TIMP1 appears also have functions that are independent of MMP inhibition, including promoting erythropoiesis and inhibiting apoptosis in B cells.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 92 %", "average": "= 89" }, { "sample": "Serum", "range": "89 - 90 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "86 - 91 %", "average": "= 89" }, { "sample": "Saliva", "range": "82 - 87 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "94 - 98 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "95 - 99 %", "average": "= 97" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
Biological function summary

TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.

Pathways

TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.

TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
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Product protocols

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Target data

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
See full target information TIMP1
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1550447 PubMed40496140

2025

Mesenchymal stem cell-derived exosome subpopulations remained consistent for 28 culture days, displaying therapeutic effects in a silicosis mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Zhang,Jing Jin,Liguang Sun,Gang Hou,Mingming Deng,Yiding Bian,Jianming Liu,Wei Cheng,Shaoliang Xing,Wenjia Wang,Xin Dong,Qingjie Fan,Lei Gao,Xinhua Lei,Yongli Bao,Yongguang Yang

Scientific reports 13:17969 PubMed37863977

2023

The combination of cigarette smoke and solar rays causes effects similar to skin aging in a bilayer skin model.

Applications

Unspecified application

Species

Unspecified reactive species

Alexe Grenier,Mathieu C Morissette,Patrick J Rochette,Roxane Pouliot

International journal of molecular sciences 23: PubMed36555389

2022

Gene Expression Linked to Reepithelialization of Human Skin Wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Magnus S Ågren,Thomas Litman,Jens Ole Eriksen,Peter Schjerling,Michael Bzorek,Lise Mette Rahbek Gjerdrum

Acta dermato-venereologica 102:adv00771 PubMed36039412

2022

Corrigendum: Biomarkers of Skin Graft Healing in Venous Leg Ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Klaus Kirketerp-Møller,Petra Doerfler,Nicole Schoefmann,Barbara Wolff-Winiski,Omid Niazi,Vibeke Pless,Tonny Karlsmark,Magnus S Ågren

Acta dermato-venereologica 102:adv00749 PubMed35604238

2022

Biomarkers of Skin Graft Healing in Venous Leg Ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Klaus Kirketerp-Møller,Petra Doerfler,Nicole Schoefmann,Barbara Wolff-Winiski,Omid Niazi,Vibeke Pless,Tonny Karlsmark,Magnus S. Ågren

Journal of oncology 2022:8319221 PubMed35847358

2022

Effects of Surgery Combined with Different Chemotherapy on Matrix Metalloproteinase-9 and Tissue Inhibitors of Metalloproteinase-1 in Children with Neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zilong Qi,Xinning Wang,Liang Yin,Kun Ma,Lei Chen,Jinmin Li

Hepatology (Baltimore, Md.) 77:558-572 PubMed35712786

2022

Circulating TREM2 as a noninvasive diagnostic biomarker for NASH in patients with elevated liver stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Vineesh Indira Chandran,Charlotte Wilhelmina Wernberg,Mette Munk Lauridsen,Maria Kløjgaard Skytthe,Sofie Marchsteiner Bendixen,Frederik Tibert Larsen,Camilla Dalby Hansen,Lea Ladegaard Grønkjær,Majken Storm Siersbæk,Tina Di Caterino,Sönke Detlefsen,Holger Jon Møller,Lars Grøntved,Kim Ravnskjaer,Søren Kragh Moestrup,Maja Sofie Thiele,Aleksander Krag,Jonas Heilskov Graversen

Veterinary ophthalmology 24:279-287 PubMed33834598

2021

Determining MMP-2 and MMP-9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Erotides Capistrano da Silva,Daniel J Gibson,Sunyoung Jeong,Kelli L Zimmerman,Kathryn M Smith-Fleming,Bianca da C Martins

BMC cardiovascular disorders 20:468 PubMed33129260

2020

Atrial matrix remodeling in atrial fibrillation patients with aortic stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Fragão-Marques,I Miranda,D Martins,I Barroso,C Mendes,A Pereira-Neves,I Falcão-Pires,A Leite-Moreira

Cellular signalling 72:109661 PubMed32334027

2020

Fibrin fragment E potentiates TGF-β-induced myofibroblast activation and recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Peder Öhman Fuchs,Carlemi Calitz,Nataša Pavlović,François Binet,Sara Marie Øie Solbak,U Helena Danielson,Johan Kreuger,Femke Heindryckx,Pär Gerwins
View all publications
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