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AB100653

Human TIMP2 ELISA Kit

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(4 Publications)

Human TIMP2 ELISA Kit is a Sandwich ELISA for the measurement of Human TIMP2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2

2 Images
Sandwich ELISA - Human TIMP2 ELISA Kit (AB100653)
  • sELISA

Unknown

Sandwich ELISA - Human TIMP2 ELISA Kit (AB100653)

Representative standard curve using ab100653

Sandwich ELISA - Human TIMP2 ELISA Kit (AB100653)
  • sELISA

Unknown

Sandwich ELISA - Human TIMP2 ELISA Kit (AB100653)

Representative standard curve using ab100653

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 2 pg/mL

Range

1.6 - 1200 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human TIMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP-2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human TIMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human TIMP2 ELISA Kit (ab188395) from our SimpleStep ELISA® range.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 103 %", "average": "= 94.53" }, { "sample": "Serum", "range": "83 - 103 %", "average": "= 94.28" }, { "sample": "Plasma", "range": "82 - 102 %", "average": "= 93.69" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100653-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100653-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100653-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human TIMP-2", "number":"AB100653-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100653-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100653-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100653-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human TIMP-2 Standard (lyophilized)", "number":"AB100653-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100653-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TIMP-2 Microplate (12 x 8 wells)", "number":"AB100653-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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Product protocols

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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
See full target information TIMP2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

BMC cardiovascular disorders 20:468 PubMed33129260

2020

Atrial matrix remodeling in atrial fibrillation patients with aortic stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Fragão-Marques,I Miranda,D Martins,I Barroso,C Mendes,A Pereira-Neves,I Falcão-Pires,A Leite-Moreira

Frontiers in immunology 9:1789 PubMed30131806

2018

Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Baharak Hosseinkhani,Sören Kuypers,Nynke M S van den Akker,Daniel G M Molin,Luc Michiels

Reproductive biology and endocrinology : RB&E 13:102 PubMed26337061

2015

Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Jui Yang,Fon-Chang Liu,Jih-Sheng Hsieh,Ching-Hung Chen,Shun-Yu Hsiao,Chih-Sheng Lin

Molecular human reproduction 20:1135-43 PubMed25189765

2014

Functional characteristics of vaginal fibroblastic cells from premenopausal women with pelvic organ prolapse.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra M Ruiz-Zapata,Manon H Kerkhof,Behrouz Zandieh-Doulabi,Hans A M Brölmann,Theo H Smit,Marco N Helder
View all publications
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websiteProtocolBooklet
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Product promise

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