Name: Human TIMP2 ELISA Kit
SKU: ab270213
Rating: 5 (1 reviews)

Human TIMP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TIMP2 with a sensitivity of 19.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

Images

ELISA - Human TIMP2 ELISA Kit (AB270213), expandable thumbnail

Publications

Key facts

Detection method: Colorimetric
Sample types: Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 78.125 - 5000 pg/mL
Assay time: 1h 30m
Sensitivity: = 19.5 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information TIMP2

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Alternative names

Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2

What's included?

1 x 96 Tests

Components

10X Human TIMP2 Capture Antibody

1 x 600 µL

10X Human TIMP2 Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

Antibody Diluent CPI2

1 x 6 mL

Human TIMP2 Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

1 x 50 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

1 x 1 Unit

Stop Solution

1 x 12 mL

TMB Development Solution

1 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 78.125 - 5000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 19.5 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Serum	n 8	C.V. 4.6

Inter assay

Sample	n	C.V.
Sample Serum	n 3	C.V. 6.4

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 107	Range 105 - 110 %
Sample type Serum	Average % = 108	Range 107 - 112 %
Sample type EDTA Plasma	Average % = 109	Range 105 - 113 %
Sample type Urine	Average % = 101	Range 96 - 105 %
Sample type Saliva	Average % = 109	Range 106 - 112 %
Sample type Cell culture media	Average % = 84	Range 82 - 85 %
Sample type Heparin Plasma	Average % = 99	Range 95 - 103 %
Sample type Citrate plasma	Average % = 111	Range 109 - 113 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human TIMP2 ELISA Kit ab270213 is a rapid single-wash 90-min Sandwich ELISA to measure Human TIMP2 in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 19.5 pg/mL.

How the assay works

Human TIMP2 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TIMP2 ELISA Kit ab270213 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TIMP2 antibody [EPR20023-43] - BSA and Azide free (Capture) Anti-TIMP2 antibody [EPR20023-112] - BSA and Azide free (Detector) ab259440
- Anti-TIMP2 antibody [EPR20023-112] - BSA and Azide free (Detector) Anti-TIMP2 antibody [EPR20023-112] - BSA and Azide free (Detector) ab259440

TIMP2 (tissue inhibitor of metalloproteinases 2) is a member of the TIMP gene family that are known to be natural inhibitors of the matrix metalloproteinases. Moreover, TIMP2 has a unique function in its ability to suppress the proliferation of endothelial cells in response to angiogenic factors as well as inhibiting protease activity in tissues undergoing extracellular matrix remodeling. As a result, making TIMP2 critical to the maintenance of tissue homeostasis.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.

Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

Associated diseases and disorders

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

ELISA - Human TIMP2 ELISA Kit (ab270213)
Sandwich ELISA - Human TIMP2 ELISA Kit (ab270213)
Human TIMP2 competitor curve comparison.
Standard Curve comparison between human TIMP2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human TIMP2 ELISA Kit (ab270213)
Human TIMP2 standard curve comparison.
Standard Curve comparison between the original Human MMP3 SimpleStep ELISA kit (ab188395) and current Human MMP3 SimpleStep ELISA kit (ab188395).
Sandwich ELISA - Human TIMP2 ELISA Kit (ab270213)
Example of human TIMP2 standard curve in Sample Diluent NS.
The TIMP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human TIMP2 ELISA Kit (ab270213)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 27,721 pg/mL with a range of 19,755 – 41,782 pg/mL.
Sandwich ELISA - Human TIMP2 ELISA Kit (ab270213)
Interpolated concentrations of native TIMP2 in human serum and plasma samples.
The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 15%, plasma (citrate) 15%, plasma (EDTA) 15%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 25,723 pg/mL in serum, 30,263 pg/mL in plasma (citrate), 22,927 pg/mL in plasma (EDTA), and 17,596 pg/mL in plasma (heparin).
Sandwich ELISA - Human TIMP2 ELISA Kit (ab270213)
Interpolated concentrations of native TIMP2 in human urine and saliva samples.
The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 100%, saliva 12.5%, HepG2 supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 3,088 pg/mL in urine, 5,618 pg/mL in saliva, and 8,400 pg/mL in HepG2 supernatant.