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AB314382

Human TIP49A ELISA Kit

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Human TIP49A ELISA Kit is a Sandwich ELISA for the measurement of Human TIP49A in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

INO80H, NMP238, TIP49, TIP49A, RUVBL1, RuvB-like 1, 49 kDa TATA box-binding protein-interacting protein, 54 kDa erythrocyte cytosolic protein, INO80 complex subunit H, Nuclear matrix protein 238, Pontin 52, TIP49a, TIP60-associated protein 54-alpha, 49 kDa TBP-interacting protein, ECP-54, NMP 238, TAP54-alpha

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Sandwich ELISA - Human TIP49A ELISA Kit (AB314382)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIP49A ELISA Kit (AB314382)

Example of standard curve using ab314382. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.45 ng/mL

Range

1.45 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB316005

Anti-TIP49A antibody [EPR28169-31] - BSA and Azide free

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP49A antibody [EPR28169-31] - BSA and Azide free (AB316005)

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Proteins & Peptides

AB98076

Recombinant Human TIP49A protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TIP49A protein (His tag N-Terminus) (AB98076)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TIP49A ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TIP49A in serum (human TIP49A concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human TIP49A coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "72 - 106 %", "average": "= 89.32" }, { "sample": "Plasma", "range": "70 - 77 %", "average": "= 72.19" }, { "sample": "Cell culture media", "range": "105 - 131 %", "average": "= 119.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314382-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314382-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314382-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314382-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human TIP49A Protein Standard", "number":"AB314382-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314382-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314382-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB314382-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human TIP49A Detection Antibody", "number":"AB314382-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human TIP49A Antibody-coated ELISA Plate", "number":"AB314382-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIP49A also known as RUVBL1 or Pontin is a 54 kDa protein that plays a mechanical role in various cellular processes. TIP49A functions as an ATPase with a part in the regulation of the chromatin structure and remodeling. It is expressed in the nucleus and cytoplasm of many cell types indicating its broad involvement in cellular activities. Being highly evolutionarily conserved TIP49A shows a strong degree of structural conservation which hints at its essential roles maintained across species.
Biological function summary

TIP49A contributes to the assembly and function of multiprotein complexes. It works closely as part of the INO80 and TIP60 chromatin remodeling complexes which are important for transcription regulation and DNA repair. Through these associations TIP49A is key in controlling access to DNA impacting gene expression and stability of chromosomes. Its role in mediating interactions between different protein partners underlines its importance in maintaining cellular homeostasis and response to DNA damage.

Pathways

TIP49A participates in essential cellular processes like transcriptional regulation and DNA repair. In the context of transcription regulation TIP49A operates alongside MYC a well-known oncogene to influence gene expression linked to cell proliferation. It also plays a significant role in the homologous recombination repair pathway interacting with proteins like RAD51 to ensure proper repair of DNA double-strand breaks. These pathways demonstrate the integral function of TIP49A in preserving genomic integrity and normal cell cycle progression.

TIP49A has links to cancer progression and neurodegenerative diseases. Overexpression of TIP49A is observed in various cancers including breast and colorectal cancer where it correlates with tumor growth and poor prognosis. It interacts with c-Myc a protein with significant oncogenic potential to drive aberrant cell proliferation. In neurodegenerative conditions such as Alzheimer's disease TIP49A's connection to chromatin remodeling dysregulation is of interest although research is still ongoing to fully elucidate this relationship.
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Product protocols

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Target data

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity (PubMed : 17157868, PubMed : 33205750). Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed : 14966270). This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription (PubMed : 14966270). This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair (PubMed : 14966270). The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage (PubMed : 14966270). Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome (PubMed : 24463511). Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding (PubMed : 16230350, PubMed : 21303910). Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex (PubMed : 10882073, PubMed : 16014379). Essential for cell proliferation (PubMed : 14506706). May be able to bind plasminogen at cell surface and enhance plasminogen activation (PubMed : 11027681).
See full target information RUVBL1
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Product promise

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