AB220653

Human Tissue Factor ELISA Kit

Recombinant
SimpleStep
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(12 Publications)

Human Tissue Factor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Tissue Factor with a sensitivity of 3.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

CD142, Tissue factor, TF, Coagulation factor III, Thromboplastin, F3

6 Images

ELISA - Human Tissue Factor ELISA Kit (AB220653)

ELISA

Supplier Data

ELISA - Human Tissue Factor ELISA Kit (AB220653)

sELISA

Supplier Data

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Interpolated concentrations of native Tissue Factor in U-87 and A431 cellular extract samples based on a 1,000 μg/mL extract load.

The concentrations of Tissue Factor were measured in duplicate and interpolated from the Tissue Factor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 10.4 ng/mL in U-87 cell extract and 100 ng/mL in A431 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Interpolated concentrations of spiked Tissue Factor in human plasma, saliva, and urine samples.

The concentrations of Tissue Factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows : 50%, plasma (citrate) 50%, plasma (heparin) 50%, saliva 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 1,000 pg/mL in neat plasma (citrate), plasma (heparin), plasma (EDTA), saliva and 2,000 pg/mL in neat urine.

sELISA

Supplier Data

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Interpolated concentrations of native Tissue Factor in human plasma and A431 cell culture supernatant samples.

The concentrations of Tissue factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, saliva 50% and A431 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 50 pg/mL in plasma (citrate), 78 pg/mL in plasma (heparin), 65.7 pg/mL in plasma (EDTA), 98 pg/mL in saliva) and 97.5 pg/mL in A431 cell culture supernatant.

sELISA

Unknown

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Example of human Tissue Factor standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Example of human Tissue Factor standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.6 pg/mL

Range

15.63 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Tissue Factor ELISA Kit ab220653 is a rapid single-wash 90-min Sandwich ELISA to measure Human Tissue Factor in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, urine. This SimpleStep sensitivity is 3.6 pg/mL.

How the assay works

Human Tissue Factor SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Tissue Factor ELISA Kit ab220653 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Tissue Factor antibody [EPR20369-66] - BSA and Azide free (Capture) ab242913
- Anti-Tissue Factor antibody [EPR20369-15] - BSA and Azide free (Detector) ab242913

Tissue factor (TF), also known as Coagulation factor III, F3 thromboplastin, and CD142 is a 263-amino acid single pass type I membrane protein encoded by the gene F3. TF is a 47 kDa single pass type I membrane protein consisting of an extracellular, transmembrane, and cytoplasmic domain. Tissue factor's main role is in blood coagulation. The protein acts as a receptor for coagulation factor VII, which then forms the active complex FVIIa. This complex proteolytically activates downstream factors, including coagulation Factor IX and X, which leads to the formation of thrombin and fibrin clot formation. Tissue Factor is in a class of proteins known as Cytokine Receptor class II family. This family is activated by cytokines, and play a role in angiogenesis and apoptosis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "105 - 116 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "111 - 118 %", "average": "= 115" }, { "sample": "Cell culture extracts", "range": "100 - 107 %", "average": "= 105" }, { "sample": "Urine", "range": "80 - 85 %", "average": "= 83" }, { "sample": "Saliva", "range": "83 - 87 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "107 - 113 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "99 - 113 %", "average": "= 104" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Factor also known as coagulation factor III or CD142 is an important player in the blood coagulation process. This protein with a molecular mass of approximately 47 kDa is a transmembrane glycoprotein. It is expressed widely in various tissues including the lungs heart and brain and plays an important role in initiating the coagulation cascade when exposed to blood. In endothelial cells tissue factor is generally not present unless these cells are in a state of disturbance supporting its role as a primary defense mechanism against vascular injury.

Biological function summary

Tissue factor acts as a cofactor for factor VIIa forming a complex that rapidly catalyzes the activation of factor X to factor Xa. This catalytic action ultimately leads to fibrin formation which is essential for clotting. Tissue Factor (TF) is part of the extrinsic pathway of the coagulation cascade and is fundamental in the generation of a hemostatic response to tissue injury. By regulating thrombin generation through its interaction with other coagulation factors tissue factor plays an important role in maintaining hemostatic balance.

Pathways

Tissue factor is integral in the extrinsic pathway of coagulation which works closely with the intrinsic pathway to ensure effective hemostasis. It complexes with factor VIIa and triggers downstream effects involving proteins like factor X and thrombin. Tissue factor forms a bridge between cellular injury responses and the blood coagulation system linking these two significant physiological processes. The interaction with these proteins highlights its central role in hemostatic pathways and highlights its significance in the coagulation network.

Tissue factor is associated with conditions like thrombosis and cardiovascular diseases where its expression can become dysregulated. Abnormal activation of tissue factor contributes directly to the pathological clot formation seen in thrombosis. It is also implicated in cancer-related coagulopathy where it can facilitate tumor-associated thrombosis by interacting with cancer cell surfaces. Additionally in such diseases the interplay between tissue factor and thrombin becomes important as it affects disease progression and the severity of clot-related complications.

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Product protocols

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Target data

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF : VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

See full target information F3

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Critical care (London, England) 28:351 PubMed39478586

2024

Effect of therapeutic plasma exchange on tissue factor and tissue factor pathway inhibitor in septic shock.

Applications

Unspecified application

Species

Unspecified reactive species

Klaus Stahl,Georg F Lehner,Pedro David Wendel-Garcia,Benjamin Seeliger,Thorben Pape,Bernhard M W Schmidt,Heiko Schenk,Julius Schmitt,Andrea Sauer,Lennart Wild,Konrad Peukert,Christian Putensen,Christian Bode,Michael Joannidis,Sascha David

Fundamental & clinical pharmacology 38:958-966 PubMed39009501

2024

Bezafibrate mitigates oxidized-low density lipoprotein (ox-LDL)-induced the attachment of monocytes to endothelial cells: An implication in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huijun Huang,Yan Shen

Scientific reports 14:14468 PubMed38914630

2024

Associations of tissue factor and tissue factor pathway inhibitor with organ dysfunctions in septic shock.

Applications

Unspecified application

Species

Unspecified reactive species

Georg Franz Lehner,Anna Katharina Tobiasch,Fabian Perschinka,Timo Mayerhöfer,Markus Waditzer,Viktoria Haller,Birgit Zassler,Sarah Maier,Hanno Ulmer,Michael Joannidis

Frontiers in immunology 14:1241448 PubMed37638055

2023

Direct endothelial ENaC activation mitigates vasculopathy induced by SARS-CoV2 spike protein.

Applications

Unspecified application

Species

Unspecified reactive species

Maritza J Romero,Qian Yue,Bhupesh Singla,Jürg Hamacher,Supriya Sridhar,Auriel S Moseley,Chang Song,Mobarak A Mraheil,Bernhard Fischer,Markus Zeitlinger,Trinad Chakraborty,David Fulton,Lin Gan,Brian H Annex,Gabor Csanyi,Douglas C Eaton,Rudolf Lucas

JCI insight 8: PubMed37227781

2023

Eculizumab treatment alters the proteometabolome beyond the inhibition of complement.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Nelke,Christina B Schroeter,Frauke Stascheit,Niklas Huntemann,Marc Pawlitzki,Alice Willison,Saskia Räuber,Nico Melzer,Ute Distler,Stefan Tenzer,Kai Stühler,Andreas Roos,Andreas Meisel,Sven G Meuth,Tobias Ruck

Cancer gene therapy 30:1355-1368 PubMed37391502

2023

CAR T cells as micropharmacies against solid cancers: Combining effector T-cell mediated cell death with vascular targeting in a one-step engineering process.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Altvater,Sareetha Kailayangiri,Christian Spurny,Maike Flügge,Jutta Meltzer,Lea Greune,Katja Urban,Christian Schwöppe,Caroline Brand,Christoph Schliemann,Heike Hintelmann,Saliha Harrach,Wolfgang Hartmann,Hinrich Abken,Johannes Kuehle,Axel Schambach,Dennis Görlich,Wolfgang E Berdel,Claudia Rossig

Research and practice in thrombosis and haemostasis 7:100133 PubMed37275179

2023

Evaluation of four commercial ELISAs to measure tissue factor in human plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Ana T A Sachetto,Sierra J Archibald,Ravi Bhatia,Dougald Monroe,Yohei Hisada,Nigel Mackman

Journal of thrombosis and haemostasis : JTH 21:629-638 PubMed36696180

2023

Peripheral blood mononuclear cell tissue factor (F3 gene) transcript levels and circulating extracellular vesicles are elevated in severe coronavirus 2019 (COVID-19) disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Girard,Lilian Antunes,Nan Zhang,Junedh M Amrute,Renumathi Subramanian,Irem Eldem,Kenneth E Remy,Monty Mazer,Emma C Erlich,Carlos Cruchaga,Ashley L Steed,Gwendalyn J Randolph,Jorge Di Paola

American journal of respiratory cell and molecular biology 64:687-697 PubMed33740387

2021

Lung Epithelial Cell Transcriptional Regulation as a Factor in COVID-19-associated Coagulopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Ethan S FitzGerald,Yongzhi Chen,Katherine A Fitzgerald,Amanda M Jamieson

Scientific reports 10:2005 PubMed32029851

2020

Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Clay T Cohen,Nancy A Turner,Joel L Moake

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Tissue Factor ELISA Kit

ELISA - Human Tissue Factor ELISA Kit (AB220653)

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Sandwich ELISA - Human Tissue Factor ELISA Kit (AB220653)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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