Human Tissue Factor ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tissue Factor in Saliva, Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma samples.
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- Frontiers in immunology 14:12414482023Direct endothelial ENaC activation mitigates vasculopathy induced by SARS-CoV2 spike protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaritza J Romero,Qian Yue,Bhupesh Singla,Jürg Hamacher,Supriya Sridhar,Auriel S Moseley,Chang Song,Mobarak A Mraheil,Bernhard Fischer,Markus Zeitlinger,Trinad Chakraborty,David Fulton,Lin Gan,Brian H Annex,Gabor Csanyi,Douglas C Eaton,Rudolf LucasPubMed 37638055
- JCI insight 8:2023Eculizumab treatment alters the proteometabolome beyond the inhibition of complement.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher Nelke,Christina B Schroeter,Frauke Stascheit,Niklas Huntemann,Marc Pawlitzki,Alice Willison,Saskia Räuber,Nico Melzer,Ute Distler,Stefan Tenzer,Kai Stühler,Andreas Roos,Andreas Meisel,Sven G Meuth,Tobias RuckPubMed 37227781
- Cancer gene therapy 30:1355-13682023CAR T cells as micropharmacies against solid cancers: Combining effector T-cell mediated cell death with vascular targeting in a one-step engineering process.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBianca Altvater,Sareetha Kailayangiri,Christian Spurny,Maike Flügge,Jutta Meltzer,Lea Greune,Katja Urban,Christian Schwöppe,Caroline Brand,Christoph Schliemann,Heike Hintelmann,Saliha Harrach,Wolfgang Hartmann,Hinrich Abken,Johannes Kuehle,Axel Schambach,Dennis Görlich,Wolfgang E Berdel,Claudia RossigPubMed 37391502
- Research and practice in thrombosis and haemostasis 7:1001332023Evaluation of four commercial ELISAs to measure tissue factor in human plasma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna T A Sachetto,Sierra J Archibald,Ravi Bhatia,Dougald Monroe,Yohei Hisada,Nigel MackmanPubMed 37275179
- Journal of thrombosis and haemostasis : JTH 21:629-6382023Peripheral blood mononuclear cell tissue factor (F3 gene) transcript levels and circulating extracellular vesicles are elevated in severe coronavirus 2019 (COVID-19) disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas J Girard,Lilian Antunes,Nan Zhang,Junedh M Amrute,Renumathi Subramanian,Irem Eldem,Kenneth E Remy,Monty Mazer,Emma C Erlich,Carlos Cruchaga,Ashley L Steed,Gwendalyn J Randolph,Jorge Di PaolaPubMed 36696180
- American journal of respiratory cell and molecular biology 64:687-6972021Lung Epithelial Cell Transcriptional Regulation as a Factor in COVID-19-associated Coagulopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEthan S FitzGerald,Yongzhi Chen,Katherine A Fitzgerald,Amanda M JamiesonPubMed 33740387
- Scientific reports 10:20052020Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClay T Cohen et. al.PubMed 32029851
- Medical science monitor : international medical jo 25:7645-76512019Expression of Long Noncoding RNA LIPCAR Promotes Cell Proliferation, Cell Migration, and Change in Phenotype of Vascular Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyan Wang et. al.PubMed 31603865
- Journal of thrombosis and haemostasis : JTH 18:306-3172019A mathematical model of coagulation under flow identifies factor V as a modifier of thrombin generation in hemophilia A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathryn G Link,Michael T Stobb,Matthew G Sorrells,Maria Bortot,Katherine Ruegg,Marilyn J Manco-Johnson,Jorge A Di Paola,Suzanne S Sindi,Aaron L Fogelson,Karin Leiderman,Keith B NeevesPubMed 31562694
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.63 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.6 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Alternative names
CD142, Tissue factor, TF, Coagulation factor III, Thromboplastin, F3
What's included?
Recommended products
Human Tissue Factor ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tissue Factor in Saliva, Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.63 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 8 | mean - | SD - | C.V. 4.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 6.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 110 | Range 105 - 116 % |
Sample type EDTA Plasma | Average % = 115 | Range 111 - 118 % |
Sample type Cell culture extracts | Average % = 105 | Range 100 - 107 % |
Sample type Urine | Average % = 83 | Range 80 - 85 % |
Sample type Saliva | Average % = 86 | Range 83 - 87 % |
Sample type Heparin Plasma | Average % = 109 | Range 107 - 113 % |
Sample type Citrate plasma | Average % = 104 | Range 99 - 113 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Tissue Factor ELISA Kit (ab220653) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tissue Factor protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tissue Factor with 3.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Tissue factor (TF), also known as Coagulation factor III, F3 thromboplastin, and CD142 is a 263-amino acid single pass type I membrane protein encoded by the gene F3. TF is a 47 kDa single pass type I membrane protein consisting of an extracellular, transmembrane, and cytoplasmic domain. Tissue factor’s main role is in blood coagulation. The protein acts as a receptor for coagulation factor VII, which then forms the active complex FVIIa. This complex proteolytically activates downstream factors, including coagulation Factor IX and X, which leads to the formation of thrombin and fibrin clot formation. Tissue Factor is in a class of proteins known as Cytokine Receptor class II family. This family is activated by cytokines, and play a role in angiogenesis and apoptosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue Factor also known as coagulation factor III or CD142 is an important player in the blood coagulation process. This protein with a molecular mass of approximately 47 kDa is a transmembrane glycoprotein. It is expressed widely in various tissues including the lungs heart and brain and plays an important role in initiating the coagulation cascade when exposed to blood. In endothelial cells tissue factor is generally not present unless these cells are in a state of disturbance supporting its role as a primary defense mechanism against vascular injury.
- Biological function summary
Tissue factor acts as a cofactor for factor VIIa forming a complex that rapidly catalyzes the activation of factor X to factor Xa. This catalytic action ultimately leads to fibrin formation which is essential for clotting. Tissue Factor (TF) is part of the extrinsic pathway of the coagulation cascade and is fundamental in the generation of a hemostatic response to tissue injury. By regulating thrombin generation through its interaction with other coagulation factors tissue factor plays an important role in maintaining hemostatic balance.
- Pathways
Tissue factor is integral in the extrinsic pathway of coagulation which works closely with the intrinsic pathway to ensure effective hemostasis. It complexes with factor VIIa and triggers downstream effects involving proteins like factor X and thrombin. Tissue factor forms a bridge between cellular injury responses and the blood coagulation system linking these two significant physiological processes. The interaction with these proteins highlights its central role in hemostatic pathways and highlights its significance in the coagulation network.
- Associated diseases and disorders
Tissue factor is associated with conditions like thrombosis and cardiovascular diseases where its expression can become dysregulated. Abnormal activation of tissue factor contributes directly to the pathological clot formation seen in thrombosis. It is also implicated in cancer-related coagulopathy where it can facilitate tumor-associated thrombosis by interacting with cancer cell surfaces. Additionally in such diseases the interplay between tissue factor and thrombin becomes important as it affects disease progression and the severity of clot-related complications.
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5 product images
Sandwich ELISA - Human Tissue Factor ELISA Kit (ab220653)
Example of human Tissue Factor standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tissue Factor ELISA Kit (ab220653)
Example of human Tissue Factor standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tissue Factor ELISA Kit (ab220653)
Interpolated concentrations of native Tissue Factor in human plasma and A431 cell culture supernatant samples.
The concentrations of Tissue factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, saliva 50% and A431 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 50 pg/mL in plasma (citrate), 78 pg/mL in plasma (heparin), 65.7 pg/mL in plasma (EDTA), 98 pg/mL in saliva) and 97.5 pg/mL in A431 cell culture supernatant.
Sandwich ELISA - Human Tissue Factor ELISA Kit (ab220653)
Interpolated concentrations of spiked Tissue Factor in human plasma, saliva, and urine samples.
The concentrations of Tissue Factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: 50%, plasma (citrate) 50%, plasma (heparin) 50%, saliva 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 1,000 pg/mL in neat plasma (citrate), plasma (heparin), plasma (EDTA), saliva and 2,000 pg/mL in neat urine.
Sandwich ELISA - Human Tissue Factor ELISA Kit (ab220653)
Interpolated concentrations of native Tissue Factor in U-87 and A431 cellular extract samples based on a 1,000 μg/mL extract load.
The concentrations of Tissue Factor were measured in duplicate and interpolated from the Tissue Factor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 10.4 ng/mL in U-87 cell extract and 100 ng/mL in A431 cell extract.
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