Human Tissue Kallikrein (KLK1) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Tissue Kallikrein (KLK1) with a sensitivity of 3.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 8.984 - 575 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.9 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. (Microbial infection) Cleaves Neisseria meningitidis NHBA in saliva; Neisseria is an obligate commensal of the nasopharyngeal mucosa.
Alternative names
Kallikrein-1, Kidney/pancreas/salivary gland kallikrein, Tissue kallikrein, KLK1
What's included?
Recommended products
Human Tissue Kallikrein (KLK1) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Tissue Kallikrein (KLK1) with a sensitivity of 3.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 8.984 - 575 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.9 pg/mL
- General recovery
- 112%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 2.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 112 | Range 104 - 117 % |
Sample type Citrate plasma | Average % = 103 | Range 99 - 110 % |
Sample type EDTA Plasma | Average % = 112 | Range 103 - 120 % |
Sample type Heparin Plasma | Average % = 99 | Range 86 - 114 % |
Sample type Urine | Average % = 104 | Range 99 - 108 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Tissue Kallikrein (KLK1) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tissue Kallikrein (KLK1) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Urine. Quantitate Human Tissue Kallikrein (KLK1) with 3.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue kallikrein also known as KLK1 is a serine protease enzyme that plays an important role in the cleavage of kininogen to produce kinins. This enzyme with a molecular weight of approximately 30 kDa exists in various tissues like the kidney pancreas and salivary glands. The protein is part of the kallikrein family and is expressed prominently in these areas. You can commonly measure its levels through ELISA assays labeled "kallikrein ELISA" or specific assays like the "kallikrein 3 ELISA."
- Biological function summary
Tissue kallikrein contributes to vasodilation and blood pressure regulation by producing bradykinin a peptide with strong vasodilator properties. It does not function alone but may interact with other enzymes to amplify its effect. Tissue kallikrein belongs to a larger family of kallikrein-related peptidases which perform various physiological roles adding complexity to its functional network within the human body.
- Pathways
This enzyme participates extensively in the kallikrein-kinin system. A significant interaction happens within the renin-angiotensin-aldosterone system contributing to blood pressure regulation fluid balance and cardiovascular function. It engages with related proteins such as kininogen and bradykinin establishing important checkpoints within these pathways.
- Associated diseases and disorders
Abnormalities in tissue kallikrein regulation link to hypertension and inflammatory diseases like rheumatoid arthritis. Dysregulation may alter interactions with proteins like kininogen leading to altered inflammatory responses or blood pressure issues. Understanding these relationships can inform therapeutic strategies targeting the kallikrein-kinin system for improved disease management.
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6 product images
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Extracellular (ab320795)
Example of human Tissue Kallikrein standard curve in Sample Diluent NS. Example of human Tissue Kallikrein standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Extracellular (ab320795)
Interpolated concentrations of human Tissue Kallikrein in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native Tissue Kallikrein was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Extracellular (ab320795)
Interpolated concentrations of human Tissue Kallikrein in urine. Interpolated concentration of native Tissue Kallikrein was measured in duplicate at different sample concentrations. Undiluted samples are 1:100 urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Extracellular (ab320795)
Interpolated concentrations of Tissue Kallikrein in normal human serum donors. Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Tissue Kallikrein concentration was determined to be 2,623 pg/mL with a range of 848 – 11,767 pg/mL.
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Extracellular (ab320795)
Example of human Tissue Kallikrein standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of human Tissue Kallikrein standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Extracellular (ab320795)
Interpolated concentrations of human Tissue Kallikrein in serum in 96-well vs. 384-well plates. Interpolated concentration of native Tissue Kallikrein was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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