AB320796

Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular

Recombinant
SimpleStep
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Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Tissue Kallikrein (KLK1) with a sensitivity of 4.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

Kallikrein-1, Kidney/pancreas/salivary gland kallikrein, Tissue kallikrein, KLK1

4 Images

Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (AB320796)

sELISA

Supplier Data

Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (AB320796)

Interpolated concentrations of human Tissue Kallikrein in human pancreas extract in 96-well vs. 384-well plates. Interpolated concentration of native Tissue Kallikrein was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2 µg/mL human pancreas extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (AB320796)

Interpolated concentrations of human Tissue Kallikrein in human pancreas tissue extract. Interpolated concentration of native Tissue Kallikrein was measured in duplicate at different sample concentrations. Undiluted samples are 3 µg/mL human pancreas tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (AB320796)

Example of human Tissue Kallikrein standard curve in 1X Cell Extraction Buffer PTR. Example of human Tissue Kallikrein standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (AB320796)

Example of human Tissue Kallikrein standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Example of human Tissue Kallikrein standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.8 pg/mL

Range

10.156 - 650 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Tissue Kallikrein (KLK1) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tissue Kallikrein (KLK1) protein in Tissue extracts. Quantitate Human Tissue Kallikrein (KLK1) with 4.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "104 - 105 %", "average": "= 105" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab320796-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB320796-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB320796-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320796-CMP10" }, { "size":"1 x 200 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320796-CMP05" }, { "size":"20 x 1 Vial", "name":"Human Tissue Kallikrein (KLK1) Lyophilized Recombinant Protein", "number":"AB320796-CMP03" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB320796-CMP04" }, { "size":"10 x 600 µL", "name":"10X Human Tissue Kallikrein (KLK1) Detector Antibody", "number":"AB320796-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human Tissue Kallikrein (KLK1) Capture Antibody", "number":"AB320796-CMP01" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB320796-CMP11" }, { "size":"1 x 50 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320796-CMP06" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab320796-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB320796-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB320796-CMP08" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB320796-CMP11" }, { "size":"1 x 20 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320796-CMP05" }, { "size":"2 x 1 Vial", "name":"Human Tissue Kallikrein (KLK1) Lyophilized Recombinant Protein", "number":"AB320796-CMP03" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB320796-CMP04" }, { "size":"1 x 600 µL", "name":"10X Human Tissue Kallikrein (KLK1) Detector Antibody", "number":"AB320796-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human Tissue Kallikrein (KLK1) Capture Antibody", "number":"AB320796-CMP01" }, { "size":"1 x 50 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320796-CMP06" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB320796-CMP12" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab320796-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB320796-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320796-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB320796-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320796-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320796-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Tissue Kallikrein (KLK1) Lyophilized Recombinant Protein", "number":"AB320796-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB320796-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Tissue Kallikrein (KLK1) Detector Antibody", "number":"AB320796-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Tissue Kallikrein (KLK1) Capture Antibody", "number":"AB320796-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB320796-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320796-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue kallikrein also known as KLK1 is a serine protease enzyme that plays an important role in the cleavage of kininogen to produce kinins. This enzyme with a molecular weight of approximately 30 kDa exists in various tissues like the kidney pancreas and salivary glands. The protein is part of the kallikrein family and is expressed prominently in these areas. You can commonly measure its levels through ELISA assays labeled "kallikrein ELISA" or specific assays like the "kallikrein 3 ELISA."

Biological function summary

Tissue kallikrein contributes to vasodilation and blood pressure regulation by producing bradykinin a peptide with strong vasodilator properties. It does not function alone but may interact with other enzymes to amplify its effect. Tissue kallikrein belongs to a larger family of kallikrein-related peptidases which perform various physiological roles adding complexity to its functional network within the human body.

Pathways

This enzyme participates extensively in the kallikrein-kinin system. A significant interaction happens within the renin-angiotensin-aldosterone system contributing to blood pressure regulation fluid balance and cardiovascular function. It engages with related proteins such as kininogen and bradykinin establishing important checkpoints within these pathways.

Abnormalities in tissue kallikrein regulation link to hypertension and inflammatory diseases like rheumatoid arthritis. Dysregulation may alter interactions with proteins like kininogen leading to altered inflammatory responses or blood pressure issues. Understanding these relationships can inform therapeutic strategies targeting the kallikrein-kinin system for improved disease management.

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Product protocols

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Target data

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.. (Microbial infection) Cleaves Neisseria meningitidis NHBA in saliva; Neisseria is an obligate commensal of the nasopharyngeal mucosa.

See full target information KLK1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular

Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (AB320796)

Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (AB320796)

Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (AB320796)

Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (AB320796)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Product promise