Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Tissue Kallikrein (KLK1) with a sensitivity of 4.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 10.156 - 650 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. (Microbial infection) Cleaves Neisseria meningitidis NHBA in saliva; Neisseria is an obligate commensal of the nasopharyngeal mucosa.
Alternative names
Kallikrein-1, Kidney/pancreas/salivary gland kallikrein, Tissue kallikrein, KLK1
What's included?
Recommended products
Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Tissue Kallikrein (KLK1) with a sensitivity of 4.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 10.156 - 650 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.8 pg/mL
- General recovery
- 105%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 1.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 1.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 105 | Range 104 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Tissue Kallikrein (KLK1) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tissue Kallikrein (KLK1) protein in Tissue extracts. Quantitate Human Tissue Kallikrein (KLK1) with 4.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue kallikrein also known as KLK1 is a serine protease enzyme that plays an important role in the cleavage of kininogen to produce kinins. This enzyme with a molecular weight of approximately 30 kDa exists in various tissues like the kidney pancreas and salivary glands. The protein is part of the kallikrein family and is expressed prominently in these areas. You can commonly measure its levels through ELISA assays labeled "kallikrein ELISA" or specific assays like the "kallikrein 3 ELISA."
- Biological function summary
Tissue kallikrein contributes to vasodilation and blood pressure regulation by producing bradykinin a peptide with strong vasodilator properties. It does not function alone but may interact with other enzymes to amplify its effect. Tissue kallikrein belongs to a larger family of kallikrein-related peptidases which perform various physiological roles adding complexity to its functional network within the human body.
- Pathways
This enzyme participates extensively in the kallikrein-kinin system. A significant interaction happens within the renin-angiotensin-aldosterone system contributing to blood pressure regulation fluid balance and cardiovascular function. It engages with related proteins such as kininogen and bradykinin establishing important checkpoints within these pathways.
- Associated diseases and disorders
Abnormalities in tissue kallikrein regulation link to hypertension and inflammatory diseases like rheumatoid arthritis. Dysregulation may alter interactions with proteins like kininogen leading to altered inflammatory responses or blood pressure issues. Understanding these relationships can inform therapeutic strategies targeting the kallikrein-kinin system for improved disease management.
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4 product images
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (ab320796)
Example of human Tissue Kallikrein standard curve in 1X Cell Extraction Buffer PTR. Example of human Tissue Kallikrein standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (ab320796)
Interpolated concentrations of human Tissue Kallikrein in human pancreas tissue extract. Interpolated concentration of native Tissue Kallikrein was measured in duplicate at different sample concentrations. Undiluted samples are 3 µg/mL human pancreas tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (ab320796)
Example of human Tissue Kallikrein standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Example of human Tissue Kallikrein standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tissue Kallikrein (KLK1) ELISA Kit- Intracellular (ab320796)
Interpolated concentrations of human Tissue Kallikrein in human pancreas extract in 96-well vs. 384-well plates. Interpolated concentration of native Tissue Kallikrein was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2 µg/mL human pancreas extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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