JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB282306

Human Tissue Plasminogen Activator ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Tissue Plasminogen Activator ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Tissue Plasminogen Activator with a sensitivity of 4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, PLAT

6 Images
Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)

Human Tissue Plasminogen Activator standard curve comparison.

Standard Curve comparison between the original Human Tissue Plasminogen Activator SimpleStep ELISA kit (ab190812) and current Human Tissue Plasminogen Activator SimpleStep ELISA kit (ab282306).

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)

Interpolated concentrations of native Tissue Plasminogen Activator in human serum and plasma samples.

The concentrations of Tissue Plasminogen Activator were measured in duplicates, interpolated from the Tissue Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, plasma (EDTA) 50% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Plasminogen Activator concentration was determined to be 2,258 pg/mL in neat serum, 3,736 pg/mL in neat plasma (citrate) 1,712 pg/mL in neat plasma (EDTA) and 2,883 pg/mL in neat plasma (heparin).

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Plasminogen Activator concentration was determined to be 1,651 pg/mL with a range of 669 – 5,244 pg/mL.

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)

Human Tissue Plasminogen Activator competitor curve comparison.

Standard Curve comparison between Human Tissue Plasminogen Activator SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)

Interpolated concentrations of native Tissue Plasminogen Activator in human milk and HUVEC cell culture supernatant samples.

The concentrations of Tissue Plasminogen Activator were measured in duplicates, interpolated from the Tissue Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 25%, and HUVEC supernatant 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Plasminogen Activator concentration was determined to be 1,063 pg/mL in neat milk and 161,985 pg/mL in neat HUVEC supernatant.

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (AB282306)

Example of human Tissue Plasminogen Activator standard curve in Sample Diluent NS.

The Tissue Plasminogen Activator standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB254502

Human alpha-2 antiplasmin ELISA Kit

Recombinant|SimpleStep

ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)

  • 0
  • 0
Assay Kits

AB108893

Human Plasminogen ELISA Kit (PLG)

Sandwich ELISA - Human Plasminogen ELISA Kit (PLG) (AB108893)

  • 5
  • 2
Assay Kits

AB226904

Human U-Plasminogen Activator ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

  • 0
  • 0
Assay Kits

AB270210

Human Thrombin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Thrombin ELISA Kit (AB270210)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Tissue Plasminogen Activator ELISA kit (ab282306) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Tissue Plasminogen Activator protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tissue Plasminogen Activator with 4 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Tissue Plasminogen Activator is a circulating serine protease involved in the breakdown of clots. Tissue Plasminogen Activator converts inactive plasminogen to active plasmin; in turn plasmin degrades the fibrin matrix in clots. In addition, plasmin can cleave Tissue Plasminogen Activator at Arg310 resulting in two chains (A and B) of Tissue Plasminogen Activator that are linked by disulfide bond. The two chains structure of Tissue Plasminogen Activator has even greater proteolytic activity. Tissue Plasminogen Activator is synthesized in many tissues and is secreted into most extracellular body fluids. Recombinant Tissue Plasminogen Activator is used medically to resolve or prevent blood clots in ischemic stroke or myocardial infarction.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 113 %", "average": "= 106" }, { "sample": "Milk", "range": "81 - 84 %", "average": "= 82" }, { "sample": "Serum", "range": "92 - 101 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "81 - 93 %", "average": "= 88" }, { "sample": "Urine", "range": "82 - 86 %", "average": "= 84" }, { "sample": "Saliva", "range": "81 - 87 %", "average": "= 84" }, { "sample": "Cell culture media", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "90 - 95 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "91 - 103 %", "average": "= 96" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282306-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282306-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282306-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282306-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282306-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282306-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282306-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Tissue Plasminogen Activator Lyophilized Recombinant Protein", "number":"AB282306-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Tissue Plasminogen Activator Capture Antibody", "number":"AB282306-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB282306-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Tissue Plasminogen Activator Detector Antibody", "number":"AB282306-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.
Biological function summary

TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.

Pathways

TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.

TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.
style
left-column

Product protocols

style
left-column

Target data

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).
See full target information PLAT
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com