Human Tissue Plasminogen Activator ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tissue Plasminogen Activator in Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
31.25 - 2000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).
Alternative names
Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, PLAT
What's included?
Recommended products
Human Tissue Plasminogen Activator ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tissue Plasminogen Activator in Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
31.25 - 2000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 106 | Range 93 - 113 % |
Sample type Milk | Average % = 82 | Range 81 - 84 % |
Sample type Serum | Average % = 97 | Range 92 - 101 % |
Sample type EDTA Plasma | Average % = 88 | Range 81 - 93 % |
Sample type Urine | Average % = 84 | Range 82 - 86 % |
Sample type Saliva | Average % = 84 | Range 81 - 87 % |
Sample type Cell culture media | Average % = 96 | Range 94 - 98 % |
Sample type Heparin Plasma | Average % = 93 | Range 90 - 95 % |
Sample type Citrate plasma | Average % = 96 | Range 91 - 103 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Tissue Plasminogen Activator ELISA kit (ab282306) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Tissue Plasminogen Activator protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tissue Plasminogen Activator with 4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Tissue Plasminogen Activator is a circulating serine protease involved in the breakdown of clots. Tissue Plasminogen Activator converts inactive plasminogen to active plasmin; in turn plasmin degrades the fibrin matrix in clots. In addition, plasmin can cleave Tissue Plasminogen Activator at Arg310 resulting in two chains (A and B) of Tissue Plasminogen Activator that are linked by disulfide bond. The two chains structure of Tissue Plasminogen Activator has even greater proteolytic activity. Tissue Plasminogen Activator is synthesized in many tissues and is secreted into most extracellular body fluids. Recombinant Tissue Plasminogen Activator is used medically to resolve or prevent blood clots in ischemic stroke or myocardial infarction.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.
- Biological function summary
TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.
- Pathways
TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.
- Associated diseases and disorders
TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.
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6 product images
Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (ab282306)
Human Tissue Plasminogen Activator competitor curve comparison.
Standard Curve comparison between Human Tissue Plasminogen Activator SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (ab282306)
Human Tissue Plasminogen Activator standard curve comparison.
Standard Curve comparison between the original Human Tissue Plasminogen Activator SimpleStep ELISA kit (Human Tissue Plasminogen Activator ELISA Kit ab190812) and current Human Tissue Plasminogen Activator SimpleStep ELISA kit (ab282306).
Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (ab282306)
Example of human Tissue Plasminogen Activator standard curve in Sample Diluent NS.
The Tissue Plasminogen Activator standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (ab282306)
Interpolated concentrations of native Tissue Plasminogen Activator in human serum and plasma samples.
The concentrations of Tissue Plasminogen Activator were measured in duplicates, interpolated from the Tissue Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 25%, plasma (EDTA) 50% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Plasminogen Activator concentration was determined to be 2,258 pg/mL in neat serum, 3,736 pg/mL in neat plasma (citrate) 1,712 pg/mL in neat plasma (EDTA) and 2,883 pg/mL in neat plasma (heparin).
Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (ab282306)
Interpolated concentrations of native Tissue Plasminogen Activator in human milk and HUVEC cell culture supernatant samples.
The concentrations of Tissue Plasminogen Activator were measured in duplicates, interpolated from the Tissue Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 25%, and HUVEC supernatant 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Plasminogen Activator concentration was determined to be 1,063 pg/mL in neat milk and 161,985 pg/mL in neat HUVEC supernatant.
Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit (ab282306)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Plasminogen Activator concentration was determined to be 1,651 pg/mL with a range of 669 – 5,244 pg/mL.
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