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AB282311

Human Tissue Plasminogen Activator ELISA Kit, Fluorescent

  • CatchPoint SimpleStep ELISA
  • Recombinant
  • SimpleStep
  • What is this?

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Human Tissue Plasminogen Activator ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Tissue Plasminogen Activator with a sensitivity of 13 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, PLAT

2 Images
Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit, Fluorescent (AB282311)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit, Fluorescent (AB282311)

Example of human Tissue Plasminogen Activator standard curve in Sample Diluent NS

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit, Fluorescent (AB282311)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tissue Plasminogen Activator ELISA Kit, Fluorescent (AB282311)

Human Tissue Plasminogen Activator standard curve comparison.

Standard Curve comparison between the original Human Tissue Plasminogen Activator Catchpoint ELISA kit (ab229408) and current Human Tissue Plasminogen Activator Catchpoint ELISA kit (ab282311).

Key facts

Detection method

Fluorescent

Sample types

Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Qualitative

Sensitivity

= 13 pg/mL

Range

31.25 - 16000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Tissue Plasminogen Activator CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Plasminogen Activator protein in human serum, plasma, milk, urine, saliva, and cell culture supernatants.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Tissue Plasminogen Activator is a circulating serine protease involved in the breakdown of clots. Tissue Plasminogen Activator converts inactive plasminogen to active plasmin; in turn plasmin degrades the fibrin matrix in clots. In addition, plasmin can cleave Tissue Plasminogen Activator at Arg310 resulting in two chains (A and B) of Tissue Plasminogen Activator that are linked by disulfide bond. The two chains structure of Tissue Plasminogen Activator has even greater proteolytic activity. Tissue Plasminogen Activator is synthesized in many tissues and is secreted into most extracellular body fluids. Recombinant Tissue Plasminogen Activator is used medically to resolve or prevent blood clots in ischemic stroke or myocardial infarction.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 113 %", "average": "= 106" }, { "sample": "Milk", "range": "81 - 84 %", "average": "= 82" }, { "sample": "Serum", "range": "92 - 101 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "81 - 93 %", "average": "= 88" }, { "sample": "Urine", "range": "82 - 86 %", "average": "= 84" }, { "sample": "Saliva", "range": "81 - 87 %", "average": "= 84" }, { "sample": "Cell culture media", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "90 - 95 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "91 - 103 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.
Biological function summary

TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.

Pathways

TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.

TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.
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Product protocols

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Target data

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).
See full target information PLAT
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websiteProtocolBooklet
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Product promise

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