AB326014

Human TL1A ELISA Kit, Chemiluminescent- Extracellular

Recombinant
SimpleStep Ignite™ ELISA
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Human TL1A ELISA Kit, Chemiluminescent- Extracellular is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human TL1A with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.29 - 5000 pg/ml with a sensitivity of 0.59 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

TL1, VEGI, TNFSF15, Tumor necrosis factor ligand superfamily member 15, TNF ligand-related molecule 1, Vascular endothelial cell growth inhibitor

5 Images

Sandwich ELISA - Human TL1A ELISA Kit, Chemiluminescent- Extracellular (AB326014)

sELISA

Lab

Sandwich ELISA - Human TL1A ELISA Kit, Chemiluminescent- Extracellular (AB326014)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Human TL1A ELISA Kit, Chemiluminescent- Extracellular (AB326014)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean TNFSF15 concentration was determined to be 35 pg/mL with a range of 19.8 – 55.5 pg/mL.

sELISA

Lab

Sandwich ELISA - Human TL1A ELISA Kit, Chemiluminescent- Extracellular (AB326014)

Standard curve comparison between the Chemiluminescent Human TNFSF15 SimpleStep ELISA and colorimetric Human TNFSF15 SimpleStep ELISA (ab317779) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human TL1A ELISA Kit, Chemiluminescent- Extracellular (AB326014)

Interpolated concentration of native TNFSF15 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HT-29 Cell Culture Supernatant 10%, and serum 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human TL1A ELISA Kit, Chemiluminescent- Extracellular (AB326014)

Dynamic range comparison between the Chemiluminescent Human TNFSF15 SimpleStep ELISA, colorimetric Human TNFSF15 SimpleStep ELISA (ab317779), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.59 pg/mL

Range

2.29 - 5000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human TL1A ELISA Kit, Chemiluminescent- Extracellular (ab326014) is a rapid single-wash sandwich 55-min ELISA to measure Human TL1A in Serum, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant with a sensitivity of 0.59 pg/mL.

How the assay works

Human TL1A ELISA Kit, Chemiluminescent- Extracellular (ab326014) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TNFSF15 (ab326014) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TNFSF15 antibody [EPR23330-106] - BSA and Aside free (Capture) ab274358
- Anti-TNFSF15 antibody [EPR23330-14] - BSA and Aside free (Detector) ab274361

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.9" } ]

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Recovery

[ { "sample": "Serum", "range": "79 - 86 %", "average": "= 82" }, { "sample": "EDTA Plasma", "range": "74 - 88 %", "average": "= 80" }, { "sample": "Heparin Plasma", "range": "80 - 84 %", "average": "= 83" }, { "sample": "Cell culture supernatant", "range": "94 - 118 %", "average": "= 106" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.

See full target information TNFSF15

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com