Human TLR2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human TLR2 in Urine, Heparin Plasma, Citrate plasma, Milk, Serum, Cell Lysate, EDTA Plasma samples.
Images
Publications
Filter by
- Journal of healthcare engineering 2022:14735972022Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Liu,Chunjing Li,Qiuyang Wang,Haiyan Hu,Chunhong Li,Jiuguang QianPubMed 35035808
- Journal of reproductive immunology 142:1031922020Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kβsignaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Yang et. al.PubMed 32950783
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Heparin Plasma, Citrate plasma, Milk, Serum, Cell Lysate, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
109.4 - 7000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 17 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
1 product for Alternative Version
Target data
Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:17889651, PubMed:21078852). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed:10426995, PubMed:10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed:11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed:16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed:16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed:15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (PubMed:16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).
Alternative names
CD282, TIL4, TLR2, Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4
What's included?
Recommended products
Human TLR2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human TLR2 in Urine, Heparin Plasma, Citrate plasma, Milk, Serum, Cell Lysate, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Heparin Plasma, Citrate plasma, Milk, Serum, Cell Lysate, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
109.4 - 7000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 17 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Milk | n 5 | mean - | SD - | C.V. 3.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Milk | n 3 | mean - | SD - | C.V. 9.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Milk | Average % = 90 | Range 88 - 94 % |
Sample type Serum | Average % = 87 | Range 84 - 90 % |
Sample type EDTA Plasma | Average % = 97 | Range 96 - 100 % |
Sample type Urine | Average % = 87 | Range 85 - 92 % |
Sample type Heparin Plasma | Average % = 89 | Range 85 - 95 % |
Sample type Citrate plasma | Average % = 86 | Range 85 - 87 % |
Sample type Cell Lysate | Average % = 100 | Range 96 - 102 % |
Sample type Cell culture media | Average % = 102 | Range 97 - 108 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human TLR2 ELISA Kit (ab227897) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TLR2 protein in cit plasma, edta plasma, hep plasma, milk, serum, urine, and cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TLR2 with 17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
The standard is affinity purified.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Toll-like receptor 2 (TLR2) also called CD282 is a protein that detects molecules from bacteria and fungi. It has a mass of about 90 kDa. TLR2 is expressed on the surface of immune cells such as monocytes macrophages and neutrophils. This receptor recognizes pathogen-associated molecular patterns (PAMPs) and activates the immune response.
- Biological function summary
TLR2 initiates signaling pathways that lead to the production of cytokines and chemokines helping to control infection. It forms dimers with other TLRs like TLR1 or TLR6 to expand its recognition range of microbial components. TLR2's activity is important for balancing pathogen recognition and avoiding overactive immune response. On macrophages TLR2 activation enhances their phagocytic ability.
- Pathways
TLR2 plays roles in innate immune signaling pathways and inflammation pathways. It contributes significantly to the NF-kB and MAPK pathways which regulate inflammation and immune response. TLR2 often cooperates with proteins like MyD88 and TRAF6 in these pathways leading to downstream activation of transcription factors and production of pro-inflammatory molecules.
- Associated diseases and disorders
TLR2 is associated with inflammatory and infectious diseases including sepsis and tuberculosis. During tuberculosis interactions between TLR2 and its ligands trigger immune responses that are important for bacterial control. Additionally TLR2’s overactivation links to sepsis by causing excessive inflammation. Researchers study TLR2 for potential therapeutic targeting in these diseases assessing how modulation can improve outcomes or reduce detrimental inflammation.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Sandwich ELISA - Human TLR2 ELISA Kit (ab227897)
Human TLR2 Competitor standard curve comparison.
Standard curve comparison between Human TLR2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
ELISA - Human TLR2 ELISA Kit (ab227897)
Example of Human TLR2 standard curve in Sample Diluent NS+ Enhancer.
Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Human TLR2 ELISA Kit (ab227897)
Example of Human TLR2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Human TLR2 ELISA Kit (ab227897)
Interpolated concentrations of spiked TLR2 in Human serum, and plasma samples.
The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
ELISA - Human TLR2 ELISA Kit (ab227897)
Interpolated concentrations of spiked TLR2 in Human cell culture media and urine samples.
The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50% and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
ELISA - Human TLR2 ELISA Kit (ab227897)
Interpolated concentrations of native TLR2 in Human milk sample.
The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 12%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR2 concentration was determined to be 41.3 ng/mL in milk.
ELISA - Human TLR2 ELISA Kit (ab227897)
Interpolated concentrations of native TLR2 in Human unstimulated THP-1 and stimulated THP-1 based on a 200 μg/mL extract load.
The concentrations of TLR2 were measured in duplicate and interpolated from the TLR2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR2 concentration was determined to be 2.3 ng/mL in unstimulated THP-1 and 6.0 ng/mL in stimulated THP-1.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com