Human TLR2 ELISA Kit (CD282) is a Sandwich (qualitative) ELISA kit for the measurement of Human TLR2 (CD282) in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
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- Physiological reports 11:e158592023Effects of acute aerobic exercise on circulating sTLR and sRAGE profiles in normal- and abnormal-glucose-tolerant individuals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan K Perkins,Edwin R Miranda,Pallavi Varshney,Sarah S Farabi,Lauretta T Quinn,Jacob M HausPubMed 37985201
- Molecular medicine reports 23:2021Involvement of TLR2/4‑MyD88‑NF‑κB signaling pathway in the pathogenesis of intracranial aneurysm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuezhi Zhang,Yilv Wan,Jiugeng Feng,Meihua Li,Zhiqun JiangPubMed 33655339
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (qualitative)
- Reactive species
- Human
- Range
- 156 - 10000 pg/mL
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:17889651, PubMed:21078852). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed:10426995, PubMed:10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed:11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed:16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed:16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed:15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (PubMed:16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).
Alternative names
CD282, TIL4, TLR2, Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4
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Recommended products
Human TLR2 ELISA Kit (CD282) is a Sandwich (qualitative) ELISA kit for the measurement of Human TLR2 (CD282) in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (qualitative)
- Reactive species
- Human
- Range
- 156 - 10000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 10 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 16 | mean 369 pg/mL | SD 18.81 | C.V. = 4.3 |
Sample Sample 3 | n 16 | mean 5303 pg/mL | SD 212.12 | C.V. = 4 |
Sample Sample 2 | n 16 | mean 2018 pg/mL | SD 133.18 | C.V. = 6.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 2 | n 24 | mean 1927 pg/mL | SD 150.3 | C.V. = 7.8 |
Sample Sample 3 | n 24 | mean 5643 pg/mL | SD 242.64 | C.V. = 4.3 |
Sample Sample 1 | n 24 | mean 392 pg/mL | SD 22.34 | C.V. = 5.7 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Human TLR2 (CD282) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human soluble TLR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
A TLR2 (CD282) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for TLR2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TLR2 amount of sample captured in plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TLR2 also known as Toll-like receptor 2 is a type 1 transmembrane protein playing a role in the innate immune system. This protein has a molecular mass of approximately 90 kDa. It is present on the surface of immune cells such as macrophages dendritic cells and some types of epithelial cells. TLR2 recognizes and binds to pathogen-associated molecular patterns acting as a receptor for lipoproteins and lipopeptides from Gram-positive bacteria mycobacteria and fungi.
- Biological function summary
TLR2 works as part of a complex with other proteins like TLR1 or TLR6 to initiate the immune response. It helps detect microbial components which leads to the activation of downstream signaling pathways and subsequent release of pro-inflammatory cytokines. TLR2 acts as an important mediator in recognizing a variety of pathogens facilitating an effective immune response.
- Pathways
TLR2 is involved in key immune signaling pathways such as the NF-kB and MAPK pathways. These pathways lead to the transcription of genes important for inflammatory responses and production of cytokines and chemokines. TLR2 interacts with other proteins like MyD88 and IRAK which are critical in propagating the signal that results in an inflammatory response tailored to combating the specific invading pathogens.
- Associated diseases and disorders
TLR2 plays a significant role in conditions like rheumatoid arthritis and sepsis. Its overactivation can lead to excessive inflammatory responses contributing to the pathogenesis of these diseases. The protein is often in connection with other receptors like TLR4 which together can amplify inflammation exacerbating disease symptoms. Understanding TLR2's role in these processes can provide insights into therapeutic targets for managing inflammatory disorders.
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3 product images
ELISA - Human TLR2 ELISA Kit (CD282) (ab131556)
Standard curve with background signal subtracted (duplicates; +/- SD).
ELISA - Human TLR2 ELISA Kit (CD282) (ab131556)
TLR2 measured in undiluted biological fluids (duplicates +/- SD).
Sandwich ELISA - Human TLR2 ELISA Kit (CD282) (ab131556)
Representative standard curve using ab131556
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