Human TLR3 ELISA Kit (CD283) is a Sandwich (qualitative) ELISA kit for the measurement of Human TLR3 (CD283) in Human in Plasma, Cell culture supernatant, Serum samples.
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- Cells 8:2019Altered Levels of Toll-Like Receptors in Circulating Extracellular Vesicles in Multiple Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavan Bhargava et. al.PubMed 31509962
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (qualitative)
- Reactive species
- Human
- Range
- 156 - 10000 pg/mL
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.
Alternative names
CD283, Toll-like receptor 3, TLR3
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Human TLR3 ELISA Kit (CD283) is a Sandwich (qualitative) ELISA kit for the measurement of Human TLR3 (CD283) in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (qualitative)
- Reactive species
- Human
- Range
- 156 - 10000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 10 pg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Human TLR3 (CD283) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human TLR3 (CD283) in cell culture supernatants, serum and plasma (heparin, EDTA).
A TLR3 (CD283) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for TLR3 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TLR3 amount of sample captured in plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TLR3 also known as Toll-like receptor 3 is a protein that plays an important role in immune response mechanisms. With a molecular mass of around 104 kDa TLR3 is part of the Toll-like receptor family and expresses mostly in immune cells including dendritic cells and macrophages. It locates mainly on the endosomal membrane where it recognizes double-stranded RNA (dsRNA) which is often a sign of viral infection. TLR3 initiates signaling cascades that lead to the activation of important immune transcription factors.
- Biological function summary
TLR3 acts as a sensor for viral and endogenous dsRNA. This receptor forms part of the innate immune system serving as a first line of defense against microbial infections. Upon encountering its ligand TLR3 undergoes conformational changes which allow interaction with the adaptor protein TRIF (TIR-domain-containing adapter-inducing interferon-β). This interaction triggers a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines which are essential for mounting an effective antiviral response.
- Pathways
TLR3 engages in important signaling cascades like the NF-kB and IRF3 pathways. These pathways are activated by the TRIF-dependent pathway which is unique among the Toll-like receptors. TLR3 influences the expression of genes involved in the inflammatory response and apoptosis. The interactions of TLR3 with other proteins such as MyD88 and IRAK1 are indirect as TLR3 does not depend on the usual MyD88-dependent pathway. This specificity makes TLR3 a critical component in pathogen recognition and response strategies within the immune system.
- Associated diseases and disorders
TLR3 has connections with several conditions primarily viral infections and autoimmune disorders like systemic lupus erythematosus (SLE). Mutations or dysregulation of TLR3 may lead to altered immune responses contributing to the pathogenesis of these diseases. TLR3's association with proteins like TRIF and IRF3 is significant in understanding its role in these conditions. Ongoing research investigates TLR3's potential as a therapeutic target to modulate immune responses in disease states.
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Sandwich ELISA - Human TLR3 ELISA Kit (CD283) (ab131557)
Representative standard curve using ab131557
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