Human TMPRSS2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TMPRSS2 with a sensitivity of 4.266 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity
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- Viruses 14:2022Expression of SARS-Cov-2 Entry Factors in Patients with Chronic Hepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Rosso,Cristina Demelas,Greta Agostini,Maria Lorena Abate,Marta Vernero,Gian Paolo Caviglia,Daphne D'Amato,Angelo Armandi,Marta Tapparo,Marta Guariglia,Giulia Troshina,Alessandro Massano,Antonella Olivero,Aurora Nicolosi,Antonella Zannetti,Rinaldo Pellicano,Alessia Ciancio,Giorgio Maria Saracco,Davide Giuseppe Ribaldone,Elisabetta Bugianesi,Sharmila FagooneePubMed 36366497
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.625 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.266 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plasma membrane-anchored serine protease that cleaves at arginine residues (PubMed:32703818, PubMed:35676539, PubMed:37990007, PubMed:38964328). Participates in proteolytic cascades of relevance for the normal physiologic function of the prostate (PubMed:25122198). Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells (PubMed:15537383, PubMed:25122198, PubMed:26018085). In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity). (Microbial infection) Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry (PubMed:24227843, PubMed:32142651, PubMed:32404436, PubMed:33051876, PubMed:34159616, PubMed:35676539, PubMed:37990007). The cleavage of SARS-COV2 spike glycoprotein occurs between the S2 and S2' site (PubMed:32703818). Upon SARS-CoV-2 infection, increases syncytia formation by accelerating the fusion process (PubMed:33051876, PubMed:34159616, PubMed:35676539). Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity. (Microbial infection) Receptor for human coronavirus HKU1-CoV, acts synergistically with disialoside glycans to facilitate the entry of the virus. After binding to cell-surface disialoside glycans, the viral S protein interacts with the inactive form of TMPRSS2 and inhibits its protease activity.
Alternative names
PRSS10, TMPRSS2, Transmembrane protease serine 2, Serine protease 10
What's included?
Recommended products
Human TMPRSS2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TMPRSS2 with a sensitivity of 4.266 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.625 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.266 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 4.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 5.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 91 | Range 83 - 96 % |
Sample type EDTA Plasma | Average % = 92 | Range 87 - 95 % |
Sample type Urine | Average % = 104 | Range 96 - 112 % |
Sample type Heparin Plasma | Average % = 94 | Range 89 - 100 % |
Sample type Citrate plasma | Average % = 95 | Range 93 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human TMPRSS2 ELISA kit (ab283552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TMRPSS2 protein in human serum, urine, cell culture supernatants, cell culture extract and tissue culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TMPRSS2 with 4.27 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human TMPRSS2 is a plasma membrane-anchored serine protease that participates in proteolytic cascades involving physiological functions of the prostate. TMPRSS2 is understood to facilitate human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms; Proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. TMPRSS2 also proteolytically cleaves and activates human coronavirus EMC (MERS), and the fusion glycoproteins F0 of Sendai virus, human metapneumovirus, human parainfluenza 1, 2, 3, 4a and 4b viruses. TMPRSS2 is essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin protein which is essential for viral infectivity. The antibodies in this product were raised to a portion of the extracellular region excluding the peptidase domain. The standard protein in this product is approximately 30% of full length TMPRSS2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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6 product images
Sandwich ELISA - Human TMPRSS2 ELISA Kit (ab283552)
Human TMPRSS2 concentration was interpolated from the TMPRSS2 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human TMPRSS2 ELISA Kit (ab283552). Wild-type and TMPRSS2 knockout LNCaP (Human TMPRSS2 knockout LNCaP cell line ab273745) cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.
Sandwich ELISA - Human TMPRSS2 ELISA Kit (ab283552)
Example of human TMPRSS2 standard curve in Sample Diluent NS.
The TMPRSS2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
Sandwich ELISA - Human TMPRSS2 ELISA Kit (ab283552)
Interpolated concentrations of spike TMPRSS2 in serum, plasma and cell culture supernatant samples.
The concentrations of TMPRSS2 were measured in duplicates and interpolated from the TMPRSS2 standard curves. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 50%. The interpolated values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human TMPRSS2 ELISA Kit (ab283552)
Interpolated concentrations of spike TMPRSS2 in 50% urine and 100% cell culture supernatant samples.
The concentrations of TMPRSS2 were measured in duplicate and interpolated from the TMPRSS2 standard curves. The interpolated values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human TMPRSS2 ELISA Kit (ab283552)
Interpolated concentrations of native TMPRSS2 in human lung tissue extract and LNCaP cell extract based on 2,000 μg/mL and 300 µg/ml extract loads, respectively.
The concentrations of TMPRSS2 were measured in duplicate and interpolated from the TMPRSS2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TMPRSS2 concentration was determined to be 166.86 pg/ml in human lung tissue extract and 909.11 pg/mL in LNCaP cell extract.
Sandwich ELISA - Human TMPRSS2 ELISA Kit (ab283552)
100% Urine from 4 bladder cancer patients was measured in duplicate.
Interpolated values are plotted (mean +/- SD, n+2). The mean native TMPRSS2 concentration was determined to be 20.27 pg/ml. *Undetected.
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