AB283552

Human TMPRSS2 ELISA Kit

KO Validated
Recombinant
SimpleStep
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(2 Publications)

Human TMPRSS2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TMPRSS2 with a sensitivity of 4.266 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity

View Alternative Names

PRSS10, TMPRSS2, Transmembrane protease serine 2, Serine protease 10

7 Images

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

sELISA

Lab

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Human TMPRSS2 concentration was interpolated from the TMPRSS2 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human TMPRSS2 ELISA Kit (ab283552). Wild-type and TMPRSS2 knockout LNCaP (ab273745) cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.

sELISA

Supplier Data

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Interpolated concentrations of spike TMPRSS2 in serum, plasma and cell culture supernatant samples.

The concentrations of TMPRSS2 were measured in duplicates and interpolated from the TMPRSS2 standard curves. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 50%. The interpolated values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Example of human TMPRSS2 standard curve in Sample Diluent NS.

The TMPRSS2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed

sELISA

Supplier Data

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Representative image of Human TMPRSS2 ELISA Kit (ab283552)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human TMPRSS2 ELISA Kit Capture Antibody

- 10X Human TMPRSS2 Detector Antibody

- 10X Human TMPRSS2 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human TMPRSS2 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Interpolated concentrations of native TMPRSS2 in human lung tissue extract and LNCaP cell extract based on 2,000 μg/mL and 300 µg/ml extract loads, respectively.

The concentrations of TMPRSS2 were measured in duplicate and interpolated from the TMPRSS2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TMPRSS2 concentration was determined to be 166.86 pg/ml in human lung tissue extract and 909.11 pg/mL in LNCaP cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Interpolated concentrations of spike TMPRSS2 in 50% urine and 100% cell culture supernatant samples.

The concentrations of TMPRSS2 were measured in duplicate and interpolated from the TMPRSS2 standard curves. The interpolated values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

100% Urine from 4 bladder cancer patients was measured in duplicate.

Interpolated values are plotted (mean +/- SD, n+2). The mean native TMPRSS2 concentration was determined to be 20.27 pg/ml. *Undetected.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.266 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human TMPRSS2 ELISA kit (ab283552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TMRPSS2 protein in human serum, urine, cell culture supernatants, cell culture extract and tissue culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TMPRSS2 with 4.27 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human TMPRSS2 is a plasma membrane-anchored serine protease that participates in proteolytic cascades involving physiological functions of the prostate. TMPRSS2 is understood to facilitate human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms; Proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. TMPRSS2 also proteolytically cleaves and activates human coronavirus EMC (MERS), and the fusion glycoproteins F0 of Sendai virus, human metapneumovirus, human parainfluenza 1, 2, 3, 4a and 4b viruses. TMPRSS2 is essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin protein which is essential for viral infectivity. The antibodies in this product were raised to a portion of the extracellular region excluding the peptidase domain. The standard protein in this product is approximately 30% of full length TMPRSS2.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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Recovery

[ { "sample": "Serum", "range": "83 - 96 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "87 - 95 %", "average": "= 92" }, { "sample": "Urine", "range": "96 - 112 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "89 - 100 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "93 - 102 %", "average": "= 95" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283552-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283552-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283552-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283552-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB283552-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283552-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283552-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283552-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB283552-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TMPRSS2 Lyophilized Recombinant Protein", "number":"AB283552-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TMPRSS2 Capture Antibody", "number":"AB283552-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB283552-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TMPRSS2 Detector Antibody", "number":"AB283552-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Plasma membrane-anchored serine protease that cleaves at arginine residues (PubMed : 32703818, PubMed : 35676539, PubMed : 37990007, PubMed : 38964328). Participates in proteolytic cascades of relevance for the normal physiologic function of the prostate (PubMed : 25122198). Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells (PubMed : 15537383, PubMed : 25122198, PubMed : 26018085). In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity).. (Microbial infection) Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry (PubMed : 24227843, PubMed : 32142651, PubMed : 32404436, PubMed : 33051876, PubMed : 34159616, PubMed : 35676539, PubMed : 37990007). The cleavage of SARS-COV2 spike glycoprotein occurs between the S2 and S2' site (PubMed : 32703818). Upon SARS-CoV-2 infection, increases syncytia formation by accelerating the fusion process (PubMed : 33051876, PubMed : 34159616, PubMed : 35676539). Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.. (Microbial infection) Receptor for human coronavirus HKU1-CoV, acts synergistically with disialoside glycans to facilitate the entry of the virus. After binding to cell-surface disialoside glycans, the viral S protein interacts with the inactive form of TMPRSS2 and inhibits its protease activity.

See full target information TMPRSS2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Viruses 17: PubMed39861814

2025

Activity of Various Cathepsin Proteases and Enrichment of Klotho Protein in the Urine and Urinary Extracellular Vesicles After SARS-CoV-2 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Niharika Bala,Ramish H Rafay,Sarah C Glover,Abdel A Alli

Viruses 14: PubMed36366497

2022

Expression of SARS-Cov-2 Entry Factors in Patients with Chronic Hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Rosso,Cristina Demelas,Greta Agostini,Maria Lorena Abate,Marta Vernero,Gian Paolo Caviglia,Daphne D'Amato,Angelo Armandi,Marta Tapparo,Marta Guariglia,Giulia Troshina,Alessandro Massano,Antonella Olivero,Aurora Nicolosi,Antonella Zannetti,Rinaldo Pellicano,Alessia Ciancio,Giorgio Maria Saracco,Davide Giuseppe Ribaldone,Elisabetta Bugianesi,Sharmila Fagoonee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human TMPRSS2 ELISA Kit

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Sandwich ELISA - Human TMPRSS2 ELISA Kit (AB283552)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Product promise