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AB326006

Human TMPRSS2 ELISA Kit, Chemiluminescent

  • Recombinant
  • SimpleStep Ignite™ ELISA
  • KO Validated
  • What is this?

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Human TMPRSS2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human TMPRSS2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.74 - 6000 pg/ml with a sensitivity of 0.83 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

PRSS10, TMPRSS2, Transmembrane protease serine 2, Serine protease 10

8 Images
Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)
  • sELISA

Lab

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)
  • sELISA

Lab

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)

Interpolated concentrations of spiked recombinant TMPRSS2 in serum, plasma and cell culture supernatant samples. The concentrations of TMPRSS2 were measured in duplicates and interpolated from the TMPRSS2 standard curves. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 50%. The interpolated values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)
  • sELISA

Lab

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)

Interpolated concentrations of spiked recombinant TMPRSS2 in 50% urine and 100% cell culture supernatant samples. The concentrations of TMPRSS2 were measured in duplicate and interpolated from the TMPRSS2 standard curves. The interpolated values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)
  • sELISA

Lab

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)

100% Urine from 4 bladder cancer patients was measured in duplicate. Interpolated values are plotted (mean +/- SD, n=2). The mean native TMPRSS2 concentration was determined to be 20.27 pg/ml. *Undetected.

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)
  • sELISA

Lab

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)

Interpolated concentrations of native TMPRSS2 in human lung tissue extract and LNCaP cell extract based on 2,000 µg/mL and 300 µg/ml extract loads, respectively. The concentrations of TMPRSS2 were measured in duplicate and interpolated from the TMPRSS2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TMPRSS2 concentration was determined to be 166.86 pg/ml in human lung tissue extract and 909.11 pg/mL in LNCaP cell extract.

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)
  • sELISA

Lab

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)

Dynamic range comparison between the Chemiluminescent Human TMPRSS2 SimpleStep ELISA, colorimetric Human TMPRSS2 SimpleStep ELISA (ab283552), and leading chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)
  • sELISA

Lab

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)

Standard curve comparison between the Chemiluminescent Human TMPRSS2 SimpleStep ELISA and colorimetric Human TMPRSS2 SimpleStep ELISA (ab283552) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)
  • sELISA

Lab

Sandwich ELISA - Human TMPRSS2 ELISA Kit, Chemiluminescent (AB326006)

Supernatants from cell culture samples were serially diluted and assessed by the Human TMPRSS2 ELISA Kit (ab283552). Wild-type and TMPRSS2 knockout LNCaP (ab273745) cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine, Cell Extract, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.83 pg/mL

Range

2.74 - 6000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TMPRSS2 ELISA Kit, Chemiluminescent (ab326006) is a rapid single-wash sandwich 55-min ELISA to measure Human TMPRSS2 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Urine, Cell Extract, Tissue Extract with a sensitivity of 0.83 pg/mL.

How the assay works

Human TMPRSS2 ELISA Kit, Chemiluminescent (ab326006) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TMPRSS2 (ab326006) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TMPRSS2 antibody [EPR24407-123] - BSA and Aside free (Capture) ab284999
- Anti-TMPRSS2 antibody [EPR24407-153] - BSA and Aside free (Detector) ab285105

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.8" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 96 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "93 - 102 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "87 - 95 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "89 - 100 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "106 - 112 %", "average": "= 109" }, { "sample": "Urine", "range": "96 - 112 %", "average": "= 104" }, { "sample": "Tissue Extracts", "range": "89 - 110 %", "average": "= 99" }, { "sample": "Cell Lysate", "range": "96 - 117 %", "average": "= 108" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Plasma membrane-anchored serine protease that cleaves at arginine residues (PubMed : 32703818, PubMed : 35676539, PubMed : 37990007, PubMed : 38964328). Participates in proteolytic cascades of relevance for the normal physiologic function of the prostate (PubMed : 25122198). Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells (PubMed : 15537383, PubMed : 25122198, PubMed : 26018085). In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity).. (Microbial infection) Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry (PubMed : 24227843, PubMed : 32142651, PubMed : 32404436, PubMed : 33051876, PubMed : 34159616, PubMed : 35676539, PubMed : 37990007). The cleavage of SARS-COV2 spike glycoprotein occurs between the S2 and S2' site (PubMed : 32703818). Upon SARS-CoV-2 infection, increases syncytia formation by accelerating the fusion process (PubMed : 33051876, PubMed : 34159616, PubMed : 35676539). Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.. (Microbial infection) Receptor for human coronavirus HKU1-CoV, acts synergistically with disialoside glycans to facilitate the entry of the virus. After binding to cell-surface disialoside glycans, the viral S protein interacts with the inactive form of TMPRSS2 and inhibits its protease activity.
See full target information TMPRSS2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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