AB100654

Human TNF alpha ELISA Kit

Name: Human TNF alpha ELISA Kit (ab100654) | Abcam
Brand: Abcam Limited
SKU: AB100654

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(16 Publications)

Human TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TNF alpha in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

5 Images

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

Representative standard curve using ab100654

sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

Representative standard curve using ab100654

sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

huTNFa measured in biological fluids, background signal subtracted (duplicates +/- SD).

sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

Standard curve of huIL-8 with background signal subtracted (duplicates; +/- SD).

sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

TNFa detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Results shown after background signal was subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 30 pg/mL

Range

24.58 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's TNF alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNF alpha in serum, plasma and cell culture supernatants. (Human TNF alpha concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA^® range.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 96.43" }, { "sample": "Serum", "range": "82 - 103 %", "average": "= 94.53" }, { "sample": "Plasma", "range": "80 - 102 %", "average": "= 91.92" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100654-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100654-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human TNF alpha Standard (lyophilized)", "number":"AB100654-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100654-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100654-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100654-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100654-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 wells)", "number":"AB100654-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human TNF alpha", "number":"AB100654-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100654-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.

See full target information TNF

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 239:e31292 PubMed38704705

2024

Primary cilia abnormalities participate in the occurrence of spontaneous abortion through TGF-β/SMAD2/3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqin Yu,Linyuan Li,Anfeng Ning,Hu Wang,Chunyi Guan,Xu Ma,Hongfei Xia

Respiratory research 24:211 PubMed37626373

2023

Prolyl oligopeptidase inhibition ameliorates experimental pulmonary fibrosis both in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Cucinotta,Deborah Mannino,Giovanna Casili,Alberto Repici,Lelio Crupi,Irene Paterniti,Emanuela Esposito,Michela Campolo

Diseases (Basel, Switzerland) 10: PubMed35892733

2022

Plasma Nickel Levels Correlate with Low Muscular Strength and Renal Function Parameters in Patients with Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Antoni Alegre-Martínez,María Isabel Martínez-Martínez,José Rubio-Briones,Omar Cauli

Cell death discovery 8:260 PubMed35568721

2022

microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guifang Zhao,Hongquan Yu,Lijuan Ding,Weiyao Wang,Huan Wang,Yao Hu,Lingsha Qin,Guangce Deng,Buqing Xie,Guofeng Li,Ling Qi

The Kaohsiung journal of medical sciences 37:1010-1015 PubMed34338425

2021

Comparison of the effect of ticagrelor combined with tirofiban versus clopidogrel combined with tirofiban on inflammation response and prognosis of patients with unstable angina pectoris in long term follow-up.

Applications

Unspecified application

Species

Unspecified reactive species

Li Deng,Hai-Zhen Jia,Mao-Chun Li,Wei Zhu

The Journal of international medical research 49:300060520985365 PubMed33845640

2021

Effect of a three-piece inflatable penile prosthesis combined with a phosphodiesterase-5 inhibitor on erectile dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Peng Wu,Qiang Liu,Liangliang Ben,Geng Chen,Zhijuan Han,Hui Peng

Oncology research 28:779-790 PubMed33741083

2021

TBK1 Inhibitor Exerts Antiproliferative Effect on Glioblastoma Multiforme Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah A Scuderi,Marika Lanza,Giovanna Casili,Francesca Esposito,Cristina Colarossi,Dario Giuffrida,Paterniti Irene,Salvatore Cuzzocrea,Emanuela Esposito,Michela Campolo

Annals of palliative medicine 9:3839-3847 PubMed33222465

2020

Nuclear factor E2 related factor (NRF2) inhibits mast cell- mediated allergic inflammation via SIRT4-mediated mitochondrial metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Binya Hu,Sijun Zhao,Min Huang,Jihao Ren

Cancers 12: PubMed32610428

2020

Interleukin-6 and Lymphocyte Count Associated and Predicted the Progression of Frailty Syndrome in Prostate Cancer Patients Undergoing Antiandrogen Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Buigues,Rut Navarro-Martínez,Vanessa Sánchez-Martínez,María Serrano-Carrascosa,José Rubio-Briones,Omar Cauli

Nutrients 12: PubMed32230954

2020

Effects of Leucine Administration in Sarcopenia: A Randomized and Placebo-controlled Clinical Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco M Martínez-Arnau,Rosa Fonfría-Vivas,Cristina Buigues,Yolanda Castillo,Pilar Molina,Aldert J Hoogland,Femke van Doesburg,Leo Pruimboom,Julio Fernández-Garrido,Omar Cauli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human TNF alpha ELISA Kit

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

Sandwich ELISA - Human TNF alpha ELISA Kit (AB100654)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (16)

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Product promise