Human TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TNF alpha in Human in Plasma, Cell culture supernatant, Serum samples.
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- Respiratory research 24:2112023Prolyl oligopeptidase inhibition ameliorates experimental pulmonary fibrosis both in vivo and in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Cucinotta,Deborah Mannino,Giovanna Casili,Alberto Repici,Lelio Crupi,Irene Paterniti,Emanuela Esposito,Michela CampoloPubMed 37626373
- Diseases (Basel, Switzerland) 10:2022Plasma Nickel Levels Correlate with Low Muscular Strength and Renal Function Parameters in Patients with Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntoni Alegre-Martínez,María Isabel Martínez-Martínez,José Rubio-Briones,Omar CauliPubMed 35892733
- Cell death discovery 8:2602022microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuifang Zhao,Hongquan Yu,Lijuan Ding,Weiyao Wang,Huan Wang,Yao Hu,Lingsha Qin,Guangce Deng,Buqing Xie,Guofeng Li,Ling QiPubMed 35568721
- The Kaohsiung journal of medical sciences 37:1010-10152021Comparison of the effect of ticagrelor combined with tirofiban versus clopidogrel combined with tirofiban on inflammation response and prognosis of patients with unstable angina pectoris in long term follow-up.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Deng,Hai-Zhen Jia,Mao-Chun Li,Wei ZhuPubMed 34338425
- The Journal of international medical research 49:3000605209853652021Effect of a three-piece inflatable penile prosthesis combined with a phosphodiesterase-5 inhibitor on erectile dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Wang,Peng Wu,Qiang Liu,Liangliang Ben,Geng Chen,Zhijuan Han,Hui PengPubMed 33845640
- Oncology research 28:779-7902021TBK1 Inhibitor Exerts Antiproliferative Effect on Glioblastoma Multiforme Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah A Scuderi,Marika Lanza,Giovanna Casili,Francesca Esposito,Cristina Colarossi,Dario Giuffrida,Paterniti Irene,Salvatore Cuzzocrea,Emanuela Esposito,Michela CampoloPubMed 33741083
- Annals of palliative medicine 9:3839-38472020Nuclear factor E2 related factor (NRF2) inhibits mast cell- mediated allergic inflammation via SIRT4-mediated mitochondrial metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBinya Hu et. al.PubMed 33222465
- Cancers 12:2020Interleukin-6 and Lymphocyte Count Associated and Predicted the Progression of Frailty Syndrome in Prostate Cancer Patients Undergoing Antiandrogen Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Buigues et. al.PubMed 32610428
- Nutrients 12:2020Effects of Leucine Administration in Sarcopenia: A Randomized and Placebo-controlled Clinical Trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco M Martínez-Arnau et. al.PubMed 32230954
- Nitric oxide : biology and chemistry 95:1-112019Saliva panel of protein candidates: A comprehensive study for assessing high altitude acclimatization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShikha Jain,Subhojit Paul,Ram Niwas Meena,Anamika Gangwar,Usha Panjwani,Yasmin Ahmad,Kalpana BhargavaPubMed 31778801
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 24.58 - 6000 pg/mL
- Sensitivity
- < 30 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:16829952, PubMed:22517918, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
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Human TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TNF alpha in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 24.58 - 6000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 30 pg/mL
- General recovery
- 94%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 96.43 | Range 84 - 104 % |
Sample type Serum | Average % = 94.53 | Range 82 - 103 % |
Sample type Plasma | Average % = 91.92 | Range 80 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's TNF alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNF alpha in serum, plasma and cell culture supernatants. (Human TNF alpha concentration is pretty low in normal serum/plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human TNF alpha ELISA Kit (Human TNF alpha ELISA Kit ab181421) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
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5 product images
Sandwich ELISA - Human TNF alpha ELISA Kit (ab100654)
Standard curve of huIL-8 with background signal subtracted (duplicates; +/- SD).
Sandwich ELISA - Human TNF alpha ELISA Kit (ab100654)
huTNFa measured in biological fluids, background signal subtracted (duplicates +/- SD).
Sandwich ELISA - Human TNF alpha ELISA Kit (ab100654)
TNFa detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) (P), and PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Results shown after background signal was subtracted (duplicates +/- SD).
Sandwich ELISA - Human TNF alpha ELISA Kit (ab100654)
Representative standard curve using ab100654
Sandwich ELISA - Human TNF alpha ELISA Kit (ab100654)
Representative standard curve using ab100654
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Product protocols
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