Human TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TNF alpha in Human in Plasma, Cell culture supernatant, Serum samples.
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- Immunity, inflammation and disease 11:e10592023The role of inflammatory factors and T-cell subsets in the diagnosis of recurrence in epithelial ovarian cancer patients and the effect of olaparin treatment on them.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGulijianati Maowulieti,Shaojie Zhao,Min Zhao,Hua YuanPubMed 37904699
- The Egyptian heart journal : (EHJ) : official bulletin of the Egyptian Society of Cardiology 75:632023Pre-procedural high serum visfatin and tumor necrosis factor-α might predict recurrent atrial fibrillation after catheter ablation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmr AlKassas,Mohamed Fouda,Gaetano Fassini,Mohamed SanhouryPubMed 37466800
- Molecular biology reports 50:5755-57652023The size of human subcutaneous adipocytes, but not adiposity, is associated with inflammation, endoplasmic reticulum stress, and insulin resistance markers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Pourdashti,Nassim Faridi,Forouzandeh Monem-Homaie,S Hamid Yaghooti,Ahmadreza Soroush,S Zahra BathaiePubMed 37219669
- Medicina (Kaunas, Lithuania) 59:2023Elevated Levels of Plasma Collagen Triple Helix Repeat Containing 1 (CTHRC1) Are Strongly Associated with eGFR and Albuminuria in Chronic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeldar Baiken,Zhannur Markhametova,Assem Ashimova,Ainur Zhulamanova,Assem Nogaibayeva,Larissa Kozina,Bakhyt Matkarimov,Bauyrzhan Aituov,Abduzhappar Gaipov,Askhat MyngbayPubMed 37109608
- Frontiers in immunology 14:10638382023T-cells engineered with a novel VHH-based chimeric antigen receptor against CD19 exhibit comparable tumoricidal efficacy to their FMC63-based counterparts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatemeh Nasiri,Pooria Safarzadeh Kozani,Fatemeh RahbarizadehPubMed 36875091
- European journal of neurology 30:3440-34502022Intestinal histomorphological and molecular alterations in patients with Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriele Bellini,Laura Benvenuti,Chiara Ippolito,Daniela Frosini,Cristina Segnani,Francesco Rettura,Andrea Pancetti,Lorenzo Bertani,Vanessa D'Antongiovanni,Giovanni Palermo,Eleonora Del Prete,Luca Antonioli,Vincenzo Nardini,Riccardo Morganti,Carolina Pellegrini,Nunzia Bernardini,Roberto Ceravolo,Matteo Fornai,Massimo BelliniPubMed 36263629
- Nutrients 14:2022Antioxidant Efficacy of a Standardized Red Orange ( (L.) Osbeck) Extract in Elderly Subjects: A Randomized, Double Blind, Controlled Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVincenzo Nobile,Marta Pisati,Enza Cestone,Violetta Insolia,Vincenzo Zaccaria,Giuseppe Antonio MalfaPubMed 36296919
- Bioengineered 13:12726-127372022Knockout of circRNA single stranded interacting protein 1 (circRBMS1) played a protective role in myocardial ischemia-reperfusion injury though inhibition of miR-2355-3p/Mammalian Sterile20-like kinase 1 (MST1) axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingping Liang,Huanhuan Jie,Qin Liu,Chang Li,Renjie Xiao,Xianliang Xing,Jing Sun,Shuchun Yu,Yanhui Hu,Guo-Hai XuPubMed 35611768
- Annals of translational medicine 10:2172022Exosomes from HPV-16 E7-pulsed dendritic cells prevent the migration, M1 polarization, and inflammation of macrophages in cervical cancer by regulating catalase 2 (CAT2).Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangping Zhang,Yanan Liao,Xiaoping Pan,Xiaoli ZhangPubMed 35280390
- The Prostate 82:531-5392022Plasma IL-6 and TNF-α levels correlate significantly with grading changes in localized prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiatong Zhou,Haojie Chen,Yanyuan Wu,Bowen Shi,Jie Ding,Jun QiPubMed 35037273
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 25 - 800 pg/mL
- Assay time
- 3h 45m
- Sensitivity
- = 8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:16829952, PubMed:22517918, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
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Human TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TNF alpha in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 25 - 800 pg/mL
- Assay time
- 3h 45m
- Assay Platform
- Microplate
- Sensitivity
- = 8 pg/mL
- General recovery
- 107%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Sample | n 6 | C.V. 3.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Sample | n 6 | C.V. 9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 81 | Range 74 - 90 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human TNF alpha ELISA kit (ab46087) is a quantitative ELISA assay for the measurement of TNF alpha in supernatants, buffered solutions, serum and plasma samples.
A monoclonal antibody specific TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant Human TNF alpha.
Get results in 90 minutes with Human TNF alpha ELISA Kit (Human TNF alpha ELISA Kit ab181421) from our SimpleStep ELISA® range.
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2 product images
Sandwich ELISA - Human TNF alpha ELISA Kit (ab46087)
Representative standard curve using ab46087
Sandwich ELISA - Human TNF alpha ELISA Kit (ab46087)
TNF? was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 μg x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) with LPS and control and stimulated PBMCs (PHA 10 μg x mL-1; 3 days). Results are shown with background signal subtracted (duplicates +/- SD), with the positive control from ab46087 included.
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