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AB46087

Human TNF alpha ELISA Kit

5

(1 Review)

|

(39 Publications)

Human TNF alpha ELISA Kit is a Sandwich ELISA for the measurement of Human TNF alpha in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

2 Images
Sandwich ELISA - Human TNF alpha ELISA Kit (AB46087)
  • sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB46087)

TNFα was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 μg x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) with LPS and control and stimulated PBMCs (PHA 10 μg x mL-1; 3 days). Results are shown with background signal subtracted (duplicates +/- SD), with the positive control from ab46087 included.

Sandwich ELISA - Human TNF alpha ELISA Kit (AB46087)
  • sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB46087)

Representative standard curve using ab46087

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8 pg/mL

Range

25 - 800 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TNF alpha ELISA kit (ab46087) is a quantitative ELISA assay for the measurement of TNF alpha in supernatants, buffered solutions, serum and plasma samples.

A monoclonal antibody specific TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.

This kit will recognize both endogenous and recombinant Human TNF alpha.

Get results in 90 minutes with Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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Recovery

[ { "sample": "Serum", "range": "74 - 90 %", "average": "= 81" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46087-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46087-CMP03" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46087-CMP13" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46087-CMP07" }, { "size":"2 x 1 Vial", "name":"TNF alpha Control", "number":"AB46087-CMP05" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46087-CMP02" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46087-CMP09" }, { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46087-CMP11" }, { "size":"1 x 2 Unit", "name":"Plastic Plate Covers", "number":"AB46087-CMP12" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46087-CMP10" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-TNF alpha", "number":"AB46087-CMP04" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46087-CMP01" }, { "size":"2 x 1 Vial", "name":"TNF alpha Standard", "number":"AB46087-CMP06" }, { "size":"1 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 well strips)", "number":"AB46087-CMP08" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46087-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46087-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46087-CMP10", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plastic Plate Covers", "number":"AB46087-CMP12", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46087-CMP01", "productcode":"" }, { "size":"4 x 1 Vial", "name":"TNF alpha Standard", "number":"AB46087-CMP06", "productcode":"" }, { "size":"2 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 well strips)", "number":"AB46087-CMP08", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46087-CMP07", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46087-CMP09", "productcode":"" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46087-CMP11", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-TNF alpha", "number":"AB46087-CMP04", "productcode":"" }, { "size":"4 x 1 Vial", "name":"TNF alpha Control", "number":"AB46087-CMP05", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46087-CMP13", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46087-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed : 16829952, PubMed : 22517918, PubMed : 23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed : 12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information TNF
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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Genes 16: PubMed40282416

2025

IL-6 Affects Liver Metabolic Abnormalities Caused by Silicon Exposure by Regulating the PKC/YY1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhao,Huihui Tao,Jian Gao,Jingjing Wang,Guangliang Hui,Ye Zhu,Jialin Wang,Xuansheng Ding,Yong Dai

PloS one 20:e0303736 PubMed40179064

2025

Elucidating the impact of Hypericum alpestre extract and L-NAME on the PI3K/Akt signaling pathway in A549 lung adenocarcinoma and MDA-MB-231 triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hayarpi Javrushyan,Mikayel Ginovyan,Tigran Harutyunyan,Smbat Gevorgyan,Zaruhi Karabekian,Alina Maloyan,Nikolay Avtandilyan

Journal of inflammation (London, England) 21:23 PubMed38907339

2024

Microcirculatory disturbance in acute liver injury is triggered by IFNγ-CD40 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Miho Kurokawa,Takeshi Goya,Motoyuki Kohjima,Masatake Tanaka,Sadahiro Iwabuchi,Shigeyuki Shichino,Satoshi Ueha,Tomonobu Hioki,Tomomi Aoyagi,Motoi Takahashi,Koji Imoto,Shigeki Tashiro,Hideo Suzuki,Masaki Kato,Shinichi Hashimoto,Hideo Matsuda,Kouji Matsushima,Yoshihiro Ogawa

Immunity, inflammation and disease 11:e1059 PubMed37904699

2023

The role of inflammatory factors and T-cell subsets in the diagnosis of recurrence in epithelial ovarian cancer patients and the effect of olaparin treatment on them.

Applications

Unspecified application

Species

Unspecified reactive species

Gulijianati Maowulieti,Shaojie Zhao,Min Zhao,Hua Yuan

The Egyptian heart journal : (EHJ) : official bulletin of the Egyptian Society of Cardiology 75:63 PubMed37466800

2023

Pre-procedural high serum visfatin and tumor necrosis factor-α might predict recurrent atrial fibrillation after catheter ablation.

Applications

Unspecified application

Species

Unspecified reactive species

Amr AlKassas,Mohamed Fouda,Gaetano Fassini,Mohamed Sanhoury

Molecular biology reports 50:5755-5765 PubMed37219669

2023

The size of human subcutaneous adipocytes, but not adiposity, is associated with inflammation, endoplasmic reticulum stress, and insulin resistance markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Pourdashti,Nassim Faridi,Forouzandeh Monem-Homaie,S Hamid Yaghooti,Ahmadreza Soroush,S Zahra Bathaie

Medicina (Kaunas, Lithuania) 59: PubMed37109608

2023

Elevated Levels of Plasma Collagen Triple Helix Repeat Containing 1 (CTHRC1) Are Strongly Associated with eGFR and Albuminuria in Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yeldar Baiken,Zhannur Markhametova,Assem Ashimova,Ainur Zhulamanova,Assem Nogaibayeva,Larissa Kozina,Bakhyt Matkarimov,Bauyrzhan Aituov,Abduzhappar Gaipov,Askhat Myngbay

Frontiers in immunology 14:1063838 PubMed36875091

2023

T-cells engineered with a novel VHH-based chimeric antigen receptor against CD19 exhibit comparable tumoricidal efficacy to their FMC63-based counterparts.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Nasiri,Pooria Safarzadeh Kozani,Fatemeh Rahbarizadeh

European journal of neurology 30:3440-3450 PubMed36263629

2022

Intestinal histomorphological and molecular alterations in patients with Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Bellini,Laura Benvenuti,Chiara Ippolito,Daniela Frosini,Cristina Segnani,Francesco Rettura,Andrea Pancetti,Lorenzo Bertani,Vanessa D'Antongiovanni,Giovanni Palermo,Eleonora Del Prete,Luca Antonioli,Vincenzo Nardini,Riccardo Morganti,Carolina Pellegrini,Nunzia Bernardini,Roberto Ceravolo,Matteo Fornai,Massimo Bellini

Nutrients 14: PubMed36296919

2022

Antioxidant Efficacy of a Standardized Red Orange ( (L.) Osbeck) Extract in Elderly Subjects: A Randomized, Double Blind, Controlled Study.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Nobile,Marta Pisati,Enza Cestone,Violetta Insolia,Vincenzo Zaccaria,Giuseppe Antonio Malfa
View all publications
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