AB309419
Human TNF-alpha ELISA Kit (For Lysates)
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(1 Publication)
Human TNF-alpha ELISA Kit (For Lysates) is a Sandwich (quantitative) ELISA for the measurement of Human TNF-alpha (For Lysates) in Human in Cell/Tissue Extracts samples.
View Alternative Names
TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human TNF-alpha ELISA Kit (For Lysates) (AB309419)
Example of standard curve using ab309419. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human TNF-alpha ELISA kit (For Lysates) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-alpha in cell lysates and tissue lysates. This assay employs an antibody specific for human TNF-alpha coated on a 96-well plate.
Recovery
[ { "sample": "Cell Lysate", "range": "67 - 79 %", "average": "= 73.17" }, { "sample": "Tissue Lysate", "range": "69 - 85 %", "average": "= 76.96" } ]
What's included?
{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309419-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309419-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer (Item D2)", "number":"AB309419-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309419-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB309419-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human TNF-alpha Antibody-coated ELISA Plate (For Lysates)", "number":"AB309419-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309419-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human TNF-alpha Detection Antibody (For Lysates)", "number":"AB309419-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human TNF-alpha Protein Standard (For Lysates)", "number":"AB309419-CMP12", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Lysis Buffer (Item J)", "number":"AB309419-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309419-CMP09", "productcode":"" } ] } } }
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Alpha psychiatry 26:38775 PubMed40110383
2025
Effect of Paliperidone Combined with Sertraline in the Treatment of Schizophrenia and its Influence on Serum Neurofunctional Related Factors.
Applications
Unspecified application
Species
Unspecified reactive species
Meng Gu,Zhilian Pi,Long Zhu,Jun Zhang
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