Human TNF beta ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TNF beta in Human in Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids samples.
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- Health science reports 6:e10732023The etiological effect and genetic risk of +252 A/G variant of TNF-β gene related to the susceptibility of urinary tract infection in a sample of Iraqi patients with type 2 diabetes: A case control study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaimaa Y Abdulfattah,Farah T SamawiPubMed 36704423
- Experimental and therapeutic medicine 15:3096-31022018Platelet-rich plasma shows beneficial effects for patients with knee osteoarthritis by suppressing inflammatory factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuilin Huang et. al.PubMed 29599843
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Sensitivity
- < 5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed:9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR (PubMed:24248355). Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
Alternative names
TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1
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Recommended products
Human TNF beta ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TNF beta in Human in Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 5 pg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Human TNF beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human TNF beta in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
A TNF beta specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for TNF beta is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TNF Beta amount of sample captured in plate.
Product promise
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Full details and terms and conditions can be found here:
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1 product image
Sandwich ELISA - Human TNF beta ELISA Kit (ab119576)
Representative Standard Curve using ab119576.
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