AB229202

Human TNF beta ELISA Kit

Recombinant
SimpleStep
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Human TNF beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TNF beta with a sensitivity of 1.84 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1

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Sandwich ELISA - Human TNF beta ELISA Kit (AB229202)

sELISA

Supplier Data

Sandwich ELISA - Human TNF beta ELISA Kit (AB229202)

Interpolated concentrations of native TNF beta in human and Rhesus Macaque PBMC cell culture supernatants.

PBMCs were either stimulated with 1.5% PHA or unstimulated for 48 hours. The concentrations of TNF beta were measured in duplicate and interpolated from the TNF beta standard curve and corrected for sample dilution. Undiluted samples are as follows : Stimulated human PBMC supernatant 25% and stimulated Rhesus Macaque PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF beta concentration was determined to be 1,660 pg/mL in the stimulated human PBMC supernatant, 1,940 pg/mL in the Rhesus Macaque stimulated PBMC supernatant, and undetectable in the unstimulated human PBMC supernatant (not shown).

sELISA

Supplier Data

Sandwich ELISA - Human TNF beta ELISA Kit (AB229202)

Interpolated concentrations of spiked TNF beta in human serum and plasma samples.

The concentrations of TNF beta were measured in duplicates, interpolated from the TNF beta standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (heparin) 25% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human TNF beta ELISA Kit (AB229202)

Example of human TNF beta standard curve in Sample Diluent NS.

The TNF beta standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human, Rhesus monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.84 pg/mL

Range

16 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human TNF beta ELISA Kit (ab229202) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNF beta protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TNF beta with 1.84 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "PBMC s/n", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "PBMC s/n", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 103 %", "average": "= 101" }, { "sample": "Serum", "range": "82 - 83 %", "average": "= 82" }, { "sample": "EDTA Plasma", "range": "80 - 82 %", "average": "= 81" }, { "sample": "Heparin Plasma", "range": "87 - 89 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "79 - 81 %", "average": "= 80" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab229202-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB229202-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB229202-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB229202-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TNF beta Capture Antibody", "number":"AB229202-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TNF beta Detector Antibody", "number":"AB229202-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB229202-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB229202-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB229202-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TNF beta Lyophilized Recombinant Protein", "number":"AB229202-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB229202-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed : 9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR (PubMed : 24248355). Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.

See full target information LTA

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human TNF beta ELISA Kit

Sandwich ELISA - Human TNF beta ELISA Kit (AB229202)

Sandwich ELISA - Human TNF beta ELISA Kit (AB229202)

Sandwich ELISA - Human TNF beta ELISA Kit (AB229202)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise