Human TNF beta ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human TNF beta in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Rhesus monkey
- Range
- 16 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.84 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed:9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR (PubMed:24248355). Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
Alternative names
TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1
What's included?
Recommended products
Human TNF beta ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human TNF beta in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Rhesus monkey
- Range
- 16 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.84 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample PBMC s/n | n 9 | mean - | SD - | C.V. 2.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample PBMC s/n | n 3 | mean - | SD - | C.V. 3.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 101 | Range 98 - 103 % |
Sample type Serum | Average % = 82 | Range 82 - 83 % |
Sample type EDTA Plasma | Average % = 81 | Range 80 - 82 % |
Sample type Heparin Plasma | Average % = 87 | Range 87 - 89 % |
Sample type Citrate plasma | Average % = 80 | Range 79 - 81 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human TNF beta ELISA Kit (ab229202) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNF beta protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TNF beta with 1.84 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Human TNF beta ELISA Kit (ab229202)
Example of human TNF beta standard curve in Sample Diluent NS.
The TNF beta standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human TNF beta ELISA Kit (ab229202)
Interpolated concentrations of spiked TNF beta in human serum and plasma samples.
The concentrations of TNF beta were measured in duplicates, interpolated from the TNF beta standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 25% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human TNF beta ELISA Kit (ab229202)
Interpolated concentrations of native TNF beta in human and Rhesus Macaque PBMC cell culture supernatants.
PBMCs were either stimulated with 1.5% PHA or unstimulated for 48 hours. The concentrations of TNF beta were measured in duplicate and interpolated from the TNF beta standard curve and corrected for sample dilution. Undiluted samples are as follows: Stimulated human PBMC supernatant 25% and stimulated Rhesus Macaque PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF beta concentration was determined to be 1,660 pg/mL in the stimulated human PBMC supernatant, 1,940 pg/mL in the Rhesus Macaque stimulated PBMC supernatant, and undetectable in the unstimulated human PBMC supernatant (not shown).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com