Human TNFRSF14 ELISA Kit

• sELISA

Unknown

Sandwich ELISA - Human TNFRSF14 ELISA Kit (AB216950)

Comparison of secreted TNFSF14 in unstimulated and PMA/PHA-stimulated Peripheral Blood Monocyte (PBMC) Cells.

PBMC cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. TNFRSF14 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated PBMC and cell culture media. Measured values were interpolated from the TNFRSF14 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 211 pg/mL in unstimulated, 744 pg/mL in stimulated, and undetectable in media.

• sELISA

Supplier Data

Sandwich ELISA - Human TNFRSF14 ELISA Kit (AB216950)

Representative image of Human TNFRSF14 ELISA Kit (ab216950)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human TNFRSF14 Capture Antibody

- 10X Human TNFRSF14 Detector Antibody

- Human TNFRSF14 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

• sELISA

Supplier Data

Sandwich ELISA - Human TNFRSF14 ELISA Kit (AB216950)

Interpolated concentrations of native TNFRSF14 in human serum and plasma samples.

The concentrations of TNFRSF14 were measured in duplicates, interpolated from the TNFRSF14 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF14 concentration was determined to be 2,507 pg/mL in serum, 2,050 pg/mL in plasma (citrate) and 1,696 pg/mL in plasma (EDTA) and 2,044 pg/mL in plasma (heparin).

• sELISA

Supplier Data

Sandwich ELISA - Human TNFRSF14 ELISA Kit (AB216950)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF14 concentration was determined to be 2413 pg/mL with a range of 1201 – 4953 pg/mL.

• sELISA

Supplier Data

Sandwich ELISA - Human TNFRSF14 ELISA Kit (AB216950)

Example of human TNFRSF14 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human TNFRSF14 ELISA Kit (AB216950)

Interpolated concentrations of native TNFRSF14 in human Raji and HL-60 cell extracts and human liver homogenate tissue extract samples.

Samples based on a 400 μg/mL extract load. The concentrations of TNFRSF14 were measured in duplicate and interpolated from the TNFRSF14 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF14 concentration was determined to be 306 pg/mL in Raji Extract, 345 pg/mL in HL-60 extract, and 809 pg/mL in Human Liver Homogenate Extract.

• sELISA

Supplier Data

Sandwich ELISA - Human TNFRSF14 ELISA Kit (AB216950)

Example of human TNFRSF14 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.