JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213840

Human TNFRSF17 ELISA Kit (BCMA)

Be the first to review this product! Submit a review

|

(1 Publication)

Human TNFRSF17 ELISA Kit (BCMA) is a Sandwich (quantitative) ELISA for the measurement of Human TNFRSF17 (BCMA) in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

CD269, BCM, BCMA, TNFRSF17, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein

1 Images
Sandwich ELISA - Human TNFRSF17 ELISA Kit (BCMA) (AB213840)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNFRSF17 ELISA Kit (BCMA) (AB213840)

Human TNFRSF17 ELISA Kit (BCMA) (ab213840) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

31.2 - 2000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Human TNFRSF17 Enzyme-Linked Immunosorbent Assay (ELISA) kit (BCMA) (ab213840) is designed for the quantitative measurement of Human TNFRSF17 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF17/BCMA has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: M1-A54) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF17/BCMA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNFRSF17/BCMA amount of sample captured in plate.

TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "158 pg/mL", "standardDeviation": "6.64", "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "614 pg/mL", "standardDeviation": "41.14", "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1358 pg/mL", "standardDeviation": "73.33", "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "134 pg/mL", "standardDeviation": "4.69", "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "642 pg/mL", "standardDeviation": "35.95", "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1286 pg/mL", "standardDeviation": "61.73", "coefficientOfVariability": "4.8" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213840-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213840-CMP10" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human TNFRSF17 antibody", "number":"AB213840-CMP08" }, { "size":"1 x 1 Unit", "name":"Anti-Human TNFRSF17 coated Microplate (12 x 8 wells)", "number":"AB213840-CMP09" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213840-CMP04" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213840-CMP07" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213840-CMP02" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213840-CMP06" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213840-CMP05" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human TNFRSF17 standard", "number":"AB213840-CMP01" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213840-CMP11" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213840-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213840-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213840-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213840-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213840-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213840-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human TNFRSF17 antibody", "number":"AB213840-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human TNFRSF17 coated Microplate (12 x 8 wells)", "number":"AB213840-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213840-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213840-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213840-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human TNFRSF17 standard", "number":"AB213840-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

The protein expressed by the TNFRSF17 gene acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. It promotes B-cell survival and plays a role in regulating humoral immunity, while also activating NF-kappa-B and JNK. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TNFRSF17
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 10:6236 PubMed32277232

2020

Analysis of the receptor BCMA as a biomarker in systemic lupus erythematosus patients.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Celeste Salazar-Camarena,Claudia Azucena Palafox-Sánchez,Alvaro Cruz,Miguel Marín-Rosales,José Francisco Muñoz-Valle
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB315340

Anti-BCMA antibody [EPR28583-5]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody [EPR28583-5] (AB315340)

  • 0
  • 0
Assay Kits

AB215539

Human IL-18 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-18 ELISA Kit (AB215539)

  • 5
  • 1
Assay Kits

AB219047

Human CXCL9 ELISA Kit

Recombinant|SimpleStep

ELISA - Human CXCL9 ELISA Kit (AB219047)

  • 5
  • 1
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com