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AB100527

Human TNFRSF18 ELISA Kit (GITR)

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Human TNFRSF18 ELISA Kit (GITR) is a Sandwich (quantitative) ELISA for the measurement of Human TNFRSF18 (GITR) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD357, AITR, GITR, UNQ319/PRO364, TNFRSF18, Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein

6 Images
ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)
  • ELISA

Lab

ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)

Standard curve in diluent A, with background signal subtracted (duplicates; +/- SD).

ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)
  • ELISA

Lab

ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)

GITR measured in PBMC lysates (RIPA; 0.02-0.1 mg of proteins tested per well) after 72 hours of culture (1e6 cells per mL) in 0.1% DMSA (CTRL), 5 ug x mL-1 PHA (Sigma) or in 50ng x mL-1 PMA (ab120297) with 1uM ionomycin (ab120116) (P/I) (duplicates +/- SD).

Sandwich ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)
  • sELISA

Unknown

Sandwich ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)

Representative standard curve using ab100527

ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)
  • ELISA

Lab

ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)

Standard curve in diluent B, with background signal subtracted (duplicates; +/- SD).

ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)
  • ELISA

Lab

ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)

GITR measured in undiluted human biological fluids (undiluted; duplicates +/- SD).

Sandwich ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)
  • sELISA

Unknown

Sandwich ELISA - Human TNFRSF18 ELISA Kit (GITR) (AB100527)

Representative standard curve using ab100527

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 15 pg/mL

Range

13.7 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TNFRSF18 (GITR) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNFRSF18 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human TNFRSF18 coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFRSF18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFRSF18 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 105 %", "average": "= 95.69" }, { "sample": "Serum", "range": "83 - 104 %", "average": "= 94.18" }, { "sample": "Plasma", "range": "82 - 102 %", "average": "= 92.75" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100527-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Recombinant Human TNFRSF18 Standard (lyophilized)", "number":"AB100527-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human TNFRSF18", "number":"AB100527-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100527-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100527-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100527-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TNFRSF18 Microplate (12 x 8 wells)", "number":"AB100527-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100527-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100527-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100527-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
See full target information TNFRSF18
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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