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AB317779

Human TNFSF15 ELISA Kit- Extracellular

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Human TNFSF15 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human TNFSF15 with a sensitivity of 11.109 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TL1, VEGI, TNFSF15, Tumor necrosis factor ligand superfamily member 15, TNF ligand-related molecule 1, Vascular endothelial cell growth inhibitor

5 Images
Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)

Interpolated concentrations of human TNFSF15 in HT-29 cell culture supernatant. Interpolated concentration of native TNFSF15 was measured in duplicate at different sample concentrations. Undiluted samples are 100% HT-29 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)

Interpolated concentrations of TNFSF15 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFSF15 concentration of detected samples was determined to be 157.2 pg/mL with a range of undetected - 276.8 pg/mL.

Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)

Example of human TNFSF15 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)

Interpolated concentrations of TNFSF15 in human atopic dermatitis serum donors. Serum of fifteen individual human atopic dermatitis donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFSF15 concentration of detected samples was determined to be 519.9 pg/mL with a range of undetected - 1,610.7 pg/mL.

Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNFSF15 ELISA Kit- Extracellular (AB317779)

Example of human TNFSF15 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture supernatant, Heparin Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11.109 pg/mL

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TNFSF15 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNFSF15 protein in Serum, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human TNFSF15 with 11.109 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Serum", "range": "79 - 86 %", "average": "= 82" }, { "sample": "EDTA Plasma", "range": "74 - 88 %", "average": "= 80" }, { "sample": "Heparin Plasma", "range": "80 - 84 %", "average": "= 83" }, { "sample": "Cell culture supernatant", "range": "94 - 118 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein TL1A also known as TNF-like ligand 1A is part of the tumor necrosis factor (TNF) superfamily. It is a cytokine with a molecular weight of approximately 23 kDa. Cells such as endothelial cells monocytes and fibroblasts express TL1A. TL1A predominantly acts as ligand for death receptor 3 (DR3) also called TNFRSF25 and is involved in immune response regulation. This protein can be found mainly in tissues involved in immune surveillance like lymph nodes and the gastrointestinal tract.
Biological function summary

TL1A interacts to induce T cell proliferation and cytokine production. It forms a complex with DR3 to activate downstream signaling pathways. These activities enhance adaptive immunity by stimulating T helper cells. Furthermore TL1A influences the production of inflammatory cytokines like interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-α). Through its interaction with DR3 TL1A modulates important immune responses.

Pathways

TL1A features in the TNF receptor-mediated signaling and the NF-kB signaling pathway. In these pathways it links to proteins such as RIPK1 and TRAF2. The signaling through DR3 influences cell survival differentiation and apoptosis. These pathways are important in maintaining the balance between immune activation and tolerance. By interacting with various signaling molecules TL1A orchestrates complex immune functions that ensure adequate immune responses and prevent autoimmunity.

Researchers connect TL1A with conditions like inflammatory bowel disease (IBD) and rheumatoid arthritis (RA). These conditions involve chronic inflammation and immune dysregulation where TL1A plays a role. In IBD elevated TL1A levels drive inflammation in the intestines. In RA TL1A potentially interacts with fibroblast-like synoviocytes contributing to joint inflammation and damage. Its relation to DR3 further implicates TL1A in these autoimmune disorders making it a target for therapeutic interventions aiming to mitigate immune-mediated damage.
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Product protocols

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Target data

Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.
See full target information TNFSF15
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websiteProtocolBooklet
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