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AB317777

Human TL1A ELISA Kit- Intracellular

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Human TL1A ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human TL1A with a sensitivity of 4.122 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TL1, VEGI, TNFSF15, Tumor necrosis factor ligand superfamily member 15, TNF ligand-related molecule 1, Vascular endothelial cell growth inhibitor

5 Images
Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)
  • sELISA

Supplier Data

Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)

Example of human TL1A standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)
  • sELISA

Supplier Data

Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)

Interpolated concentrations of human TL1A in PMA-treated HL-60 cell extract and untreated HL-60 cell extract. HL-60 cells were treated with 200 nM PMA for 24 hours or left untreated. Interpolated concentration of native TL1A was measured in duplicate at different sample concentrations. Undiluted samples are 100 µg/mL PMA-treated HL-60 cell extract and 100 µg/mL untreated HL-60 cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR. No signal was detected in untreated HL-60 cell extract.

Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)
  • sELISA

Supplier Data

Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)

Example of human TL1A standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)
  • sELISA

Supplier Data

Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)

Interpolated concentrations of human TL1A in PMA-treated HL-60 cell extract in 96-well vs. 384-well plates. HL-60 cells were treated with 200 nM PMA for 24 hours. Interpolated concentration of native TL1A was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100 µg/mL PMA-treated HL-60 cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)
  • sELISA

Supplier Data

Sandwich ELISA - Human TL1A ELISA Kit- Intracellular (AB317777)

Representative image of Human TL1A ELISA Kit- Intracellular (ab317777)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human TNFSF15- Intracellular Capture Antibody

- 10X Human TNFSF15- Intracellular Detector Antibody

- Human TNFSF15- Intracellular Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.122 pg/mL

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB274358

Anti-TL1A antibody [EPR23330-106] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-TL1A antibody [EPR23330-106] - BSA and Azide free (Capture) (AB274358)

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Proteins & Peptides

AB107131

Recombinant Human TL1A protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TL1A protein (His tag N-Terminus) (AB107131)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TL1A SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TL1A protein in Cell Lysate, Tissue extracts. Quantitate Human TL1A with 4.122 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "95 - 107 %", "average": "= 100" }, { "sample": "Tissue Extracts", "range": "85 - 97 %", "average": "= 90" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317777-10x96Tests", "assetComponentDetails": [ { "size":"20 x 1 Vial", "name":"Human TL1A Lyophilized Recombinant Protein", "number":"AB317777-CMP09" }, { "size":"10 x 600 µL", "name":"10X Human TL1A Capture Antibody", "number":"AB317777-CMP01" }, { "size":"10 x 600 µL", "name":"10X Human TL1A Detector Antibody", "number":"AB317777-CMP08" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB317777-CMP12" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317777-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317777-CMP03" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB317777-CMP10" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317777-CMP13" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317777-CMP05" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317777-CMP11" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB317777-CMP06" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317777-1x384Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human TL1A Lyophilized Recombinant Protein", "number":"AB317777-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human TL1A Capture Antibody", "number":"AB317777-CMP01" }, { "size":"1 x 600 µL", "name":"10X Human TL1A Detector Antibody", "number":"AB317777-CMP08" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB317777-CMP12" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317777-CMP04" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317777-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317777-CMP13" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB317777-CMP10" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317777-CMP07" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317777-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317777-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317777-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human TL1A Lyophilized Recombinant Protein", "number":"AB317777-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TL1A Capture Antibody", "number":"AB317777-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TL1A Detector Antibody", "number":"AB317777-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317777-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB317777-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317777-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317777-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317777-CMP13", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB317777-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317777-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317777-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317777-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein TL1A also known as TNF-like ligand 1A is part of the tumor necrosis factor (TNF) superfamily. It is a cytokine with a molecular weight of approximately 23 kDa. Cells such as endothelial cells monocytes and fibroblasts express TL1A. TL1A predominantly acts as ligand for death receptor 3 (DR3) also called TNFRSF25 and is involved in immune response regulation. This protein can be found mainly in tissues involved in immune surveillance like lymph nodes and the gastrointestinal tract.
Biological function summary

TL1A interacts to induce T cell proliferation and cytokine production. It forms a complex with DR3 to activate downstream signaling pathways. These activities enhance adaptive immunity by stimulating T helper cells. Furthermore TL1A influences the production of inflammatory cytokines like interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-α). Through its interaction with DR3 TL1A modulates important immune responses.

Pathways

TL1A features in the TNF receptor-mediated signaling and the NF-kB signaling pathway. In these pathways it links to proteins such as RIPK1 and TRAF2. The signaling through DR3 influences cell survival differentiation and apoptosis. These pathways are important in maintaining the balance between immune activation and tolerance. By interacting with various signaling molecules TL1A orchestrates complex immune functions that ensure adequate immune responses and prevent autoimmunity.

Researchers connect TL1A with conditions like inflammatory bowel disease (IBD) and rheumatoid arthritis (RA). These conditions involve chronic inflammation and immune dysregulation where TL1A plays a role. In IBD elevated TL1A levels drive inflammation in the intestines. In RA TL1A potentially interacts with fibroblast-like synoviocytes contributing to joint inflammation and damage. Its relation to DR3 further implicates TL1A in these autoimmune disorders making it a target for therapeutic interventions aiming to mitigate immune-mediated damage.
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Product protocols

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Target data

Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.
See full target information TNFSF15
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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