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AB113323

Human TNFSF18 / GITRL ELISA Kit

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Human TNFSF18 / GITRL ELISA Kit is a Sandwich ELISA for the measurement of Human TNFSF18 / GITRL in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

AITRL, GITRL, TL6, UNQ149/PRO175, TNFSF18, Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand, Glucocorticoid-induced TNF-related ligand, hGITRL

1 Images
Sandwich ELISA - Human TNFSF18 / GITRL ELISA Kit (AB113323)
  • sELISA

Unknown

Sandwich ELISA - Human TNFSF18 / GITRL ELISA Kit (AB113323)

Representative standard curve using ab113323.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 25 pg/mL

Range

41 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TNFSF18 / GITRL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNFSF18 / GITRL in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for human TNFSF18 / GITRL coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFSF18 / GITRL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFSF18 / GITRL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFSF18 / GITRL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "67 - 100 %", "average": "= 87.83" }, { "sample": "Serum", "range": "108 - 123 %", "average": "= 116.9" }, { "sample": "Plasma", "range": "67 - 100 %", "average": "= 87.69" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113323-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113323-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113323-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB113323-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113323-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human GITR Ligand Standard (lyophilized)", "number":"AB113323-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human GITR Ligand", "number":"AB113323-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TNFSF18 Ligand Microplate (12 x 8 wells)", "number":"AB113323-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113323-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB113323-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (PubMed : 23892569). Promotes leukocyte adhesion to endothelial cells (PubMed : 23892569). Regulates migration of monocytes from the splenic reservoir to sites of inflammation (By similarity).
See full target information TNFSF18
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Product promise

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