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Human TNFSF4 ELISA Kit (OX40L) is a Sandwich (quantitative) ELISA kit for the measurement of Human TNFSF4 (OX40L) in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.

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Sandwich ELISA - Human TNFSF4 ELISA Kit (OX40L) (AB213842), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
156 - 10000 pg/mL
Assay time
3h 30m
Sensitivity
< 10 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.

Alternative names

CD252, TXGP1, TNFSF4, Tumor necrosis factor ligand superfamily member 4, Glycoprotein Gp34, OX40 ligand, TAX transcriptionally-activated glycoprotein 1, OX40L

What's included?

1 x 96 Tests
Components
ABC Diluent Buffer
1 x 12 mL
Adhesive Plate Seal
4 x 1 Unit
Anti-Human TNFSF4 coated Microplate (12 x 8 wells)
1 x 1 Unit
Antibody Diluent Buffer
1 x 12 mL
Avidin-Biotin-Peroxidase Complex (ABC)
1 x 100 µL
Biotinylated anti-Human TNFSF4 antibody
1 x 100 µL
Lyophilized recombinant Human TNFSF4 standard
2 x 1 Vial
Sample Diluent Buffer
1 x 30 mL
TMB Color Developing Agent
1 x 10 mL
TMB Stop Solution
1 x 10 mL
Wash Buffer (25X)
1 x 20 mL

Recommended products

Human TNFSF4 ELISA Kit (OX40L) is a Sandwich (quantitative) ELISA kit for the measurement of Human TNFSF4 (OX40L) in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
156 - 10000 pg/mL
Assay time
3h 30m
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
< 10 pg/mL

Precision

Intra assay

Sample
Sample 2
n
16
mean
1225 pg/mL
SD
51.45
C.V.
= 4.2
Sample
Sample 3
n
16
mean
4641 pg/mL
SD
305.7
C.V.
= 6.6
Sample
Sample 1
n
16
mean
209 pg/mL
SD
8.56
C.V.
= 4.1

Inter assay

Sample
Sample 3
n
24
mean
4885 pg/mL
SD
365.77
C.V.
= 7.5
Sample
Sample 2
n
24
mean
1277 pg/mL
SD
72.78
C.V.
= 5.7
Sample
Sample 1
n
24
mean
228 pg/mL
SD
12.54
C.V.
= 5.5

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

The Human TNFSF4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (OX40L) (ab213842) is designed for the quantitative measurement of Human TNFSF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF4/OX40L has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q51-L183) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF4/OX40L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNFSF4/OX40L amount of sample captured in plate.

OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

OX40L also known as TNFSF4 is a member of the tumor necrosis factor (TNF) ligand family. It is a type II transmembrane protein with a molecular weight of about 32 kDa. OX40L is expressed mainly on activated antigen-presenting cells such as B cells dendritic cells and macrophages. The protein interacts specifically with its receptor OX40 (CD134) which is found on activated T cells mainly CD4+ T cells. These interactions are important for the regulation of immune responses.

Biological function summary

OX40L acts as a costimulatory signal for T cells. It does not work alone; it functions as part of the immune complex involved in enhancing T cell proliferation cytokine production and survival. OX40L enhances the immune response making it a critical modulator of immunity. This protein interaction is essential for promoting an effective adaptive immune response influencing T cell memory and maintaining immune system balance.

Pathways

OX40L is involved in the TNF receptor pathway which plays an important role in cell survival and inflammation. It influences the NF-κB signaling pathway which regulates immune response and cell proliferation. OX40L interactions also involve the Akt pathway important for T cell survival and function. The related proteins in these pathways include notably the OX40 protein and CD317 which participate in immune signaling and regulation.

Associated diseases and disorders

OX40L is implicated in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The interaction between OX40L and OX40 increases T cell activation contributing to inflammation and disease progression. OX40L's excessive activity has links to these pathologies making it a potential therapeutic target. Another related protein within disease contexts is CD317 connected to immune modulation and control of viral infections and offering further opportunities for targeted treatment strategies involving OX40L.

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  • Sandwich ELISA - Human TNFSF4 ELISA Kit (OX40L) (ab213842), expandable thumbnail

    Sandwich ELISA - Human TNFSF4 ELISA Kit (OX40L) (ab213842)

    Human TNFSF4 ELISA Kit (OX40L) (ab213842) Standard Curve.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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