Human TNR ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TNR in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Sensitivity
- = 0.2 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance (By similarity).
Alternative names
Tenascin-R, TN-R, Janusin, Restrictin, TNR
What's included?
Recommended products
Human TNR ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TNR in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.2 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 94.59 | Range 76 - 122 % |
Sample type Plasma | Average % = 103.4 | Range 78 - 141 % |
Sample type Cell culture media | Average % = 108.4 | Range 88 - 130 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human TNR ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNR in serum (human TNR concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma (collect plasma using citrate and heparin as an anticoagulant. EDTA is not recommended as anticoagulants for use in this assay) and cell culture supernatants. This assay employs an antibody specific for human TNR coated on a 96-well plate.
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1 product image
Sandwich ELISA - Human TNR ELISA Kit (ab314706)
Example of standard curve using ab314706. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
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