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AB324318

Human Topoisomerase II beta/TOP2B ELISA Kit- Extracellular

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Human Topoisomerase II beta/TOP2B ELISA Kit- Extracellular is a Sandwich (quantitative) ELISA for the measurement of Human Topoisomerase II beta/TOP2B - Extracellular in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

DNA topoisomerase 2-beta, TOP2B

2 Images
Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Extracellular (AB324318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Extracellular (AB324318)

Example of human Topoisomerase II beta/TOP2B standard curve in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Extracellular (AB324318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Extracellular (AB324318)

Example of human Topoisomerase II beta/TOP2B standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 25.253 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Topoisomerase II beta/TOP2B SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Topoisomerase II beta/TOP2B protein in Serum, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human Topoisomerase II beta/TOP2B with 25.253 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.9" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.1" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 93 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "87 - 99 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "90 - 95 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "86 - 94 %", "average": "= 91" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. Plays a role in B-cell differentiation.
See full target information DNA topoisomerase 2-beta
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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