Human Total Lambda Light Chain ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Total Lambda Light Chain in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 18.75 - 1200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.42 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin lambda constant 1, Ig lambda chain C region MGC, Ig lambda-1 chain C region, IGLC1
What's included?
Human Total Lambda Light Chain ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Total Lambda Light Chain in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 18.75 - 1200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 7.42 pg/mL
- General recovery
- 100%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 6.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 7.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 100 | Range 98 - 103 % |
Sample type Citrate plasma | Average % = 95 | Range 89 - 103 % |
Sample type EDTA Plasma | Average % = 98 | Range 83 - 109 % |
Sample type Heparin Plasma | Average % = 98 | Range 92 - 104 % |
Sample type Cell culture supernatant | Average % = 107 | Range 98 - 120 % |
Sample type Urine | Average % = 100 | Range 92 - 116 % |
Sample type Saliva | Average % = 102 | Range 88 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Total Lambda Light Chain SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Total Lambda Light Chain protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Urine, Saliva. Quantitate Human Total Lambda Light Chain with 7.42 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples -96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Sandwich ELISA - Human Total Lambda Light Chain ELISA Kit (ab322363)
Interpolated concentration of native Lambda Light Chain was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:100,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Total Lambda Light Chain ELISA Kit (ab322363)
Example of human Laminin alpha 4/LAMA4 standard curve in Sample Diluent 50BS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Total Lambda Light Chain ELISA Kit (ab322363)
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Total Lambda Light Chain concentration was determined to be 103.3 µg/mL with a range of 64.6 – 131.5 µg/mL.
Sandwich ELISA - Human Total Lambda Light Chain ELISA Kit (ab322363)
Interpolated concentration of native Lambda Light Chain was measured in duplicate at different sample concentrations. Undiluted samples are as follows: urine 1:400 and saliva 1:2,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Total Lambda Light Chain ELISA Kit (ab322363)
Interpolated concentration of native Lambda Light Chain was measured in duplicate at different sample concentrations. Undiluted samples are 10% stimulated PBMC supernatant (1.5% PHA-M for 46 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Total Lambda Light Chain ELISA Kit (ab322363)
Interpolated concentration of native Lambda Light Chain was measured in duplicate at different sample concentrations. Undiluted samples are 1:100,000 human serum, plasma – citrate, plasma – EDTA, and plasma – heparin. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Total Lambda Light Chain ELISA Kit (ab322363)
Example of human Lambda Light Chain standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
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