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AB108776

Human Anti-Toxoplasma gondii IgG ELISA Kit

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(4 Publications)

Human Anti-Toxoplasma gondii IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Toxoplasma gondii in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included
1 Images
Indirect ELISA - Human Anti-Toxoplasma gondii IgG ELISA Kit (AB108776)
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Indirect ELISA - Human Anti-Toxoplasma gondii IgG ELISA Kit (AB108776)

Representative Standard Curve using ab108776

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Sensitivity

< 1 IU/mL

Range

1 - 200 U/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Toxoplasma gondii IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Toxoplasma gondii in Human serum and plasma.

A 96-well plate has been precoated with Toxoplasma gondii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Toxoplasma gondii-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxoplasma gondii IgG sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Standard B", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Inter", "sample": "Standard C", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Inter", "sample": "Standard D", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Intra", "sample": "Standard B", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "Standard C", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Intra", "sample": "Standard D", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108776-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Toxoplasma gondii IgG Standard A – 0 U/mL", "number":"AB108776-CMP07", "productcode":"" }, { "size":"1 x 2 mL", "name":"Toxoplasma gondii IgG Standard D – 200 U/mL", "number":"AB108776-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"Toxoplasma gondii IgG Standard B – 50 U/mL", "number":"AB108776-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Toxoplasma gondii (IgG) Coated Microplate (12 x 8 wells)", "number":"AB108776-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108776-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108776-CMP03", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108776-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108776-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108776-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"Toxoplasma gondii anti-IgG HRP Conjugate", "number":"AB108776-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"Toxoplasma gondii IgG Standard C – 100 U/mL", "number":"AB108776-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108776-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toxoplasma gondii IgG also known as anti-Toxoplasma IgG is an antibody that plays a significant role in the immune response against the protozoan parasite Toxoplasma gondii. This immunoglobulin has a molecular mass of approximately 150 kDa and is primarily expressed in the bloodstream during Toxoplasma infection. The detection of Toxoplasma gondii IgG serves as an important marker in serological tests used to determine past infection or exposure to this parasite.
Biological function summary

Toxoplasma gondii IgG helps mediate the immune response by binding to the surface antigens of the parasite aiding in its identification and destruction by immune cells. This interaction is facilitated by the high avidity of the IgG enhancing its ability to remain bound to Toxoplasma antigens under challenging conditions. Toxoplasma gondii IgG is not part of a larger protein complex but instead operates as a standalone molecule in the immune defense system.

Pathways

Toxoplasma gondii IgG operates within the adaptive immune pathway a critical component of immune system functioning. This pathway involves other proteins such as major histocompatibility complex (MHC) molecules and T-cell receptors which play roles in antigen presentation and cellular immune response. The interplay of these molecular components enables the immune system to effectively target and respond to Toxoplasma gondii.

Toxoplasma gondii IgG is primarily associated with toxoplasmosis an infectious disease caused by Toxoplasma gondii. In immunocompromised individuals or during pregnancy this infection can lead to severe complications. During disease progression IgG levels are often measured as part of diagnosis and monitoring. Additionally research has explored its role in the context of congenital toxoplasmosis where IgG avidity tests aid in differentiating recent infections from past ones providing insights into disease management.
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Parasitology international 84:102402 PubMed34119685

2021

Seroprevalence and risk factors of Toxoplasma gondii infection among veterinary personnel and abattoir workers in Central India.

Applications

Unspecified application

Species

Unspecified reactive species

Abhijit S Deshmukh,Bhavana K Hebbar,Pallabi Mitra,Shilpshri Shinde,Sandeep Chaudhari,Sukhadeo B Barbuddhe

Tropical biomedicine 37:1038-1049 PubMed33612756

2021

Seroprevalence of low avidity anti-Toxoplasma IgG in pregnant women and its relationship with their age and contact with cats.

Applications

Unspecified application

Species

Unspecified reactive species

K Khan,W Khan,T Khan,G Naaz,A Naheda,S Aqeel

Talanta 195:699-705 PubMed30625604

2019

Novel electrochemical sensing platform based on a nanocomposite of PVA/PVP/RGO applied to IgG anti- Toxoplasma gondii antibodies quantitation.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo A Takara,Sirley V Pereira,María L Scala-Benuzzi,Martín A Fernández-Baldo,Julio Raba,Germán A Messina

Maxillofacial plastic and reconstructive surgery 36:192-200 PubMed27489833

2014

Soft Tissue Augmentation with Silk Composite Graft.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Tae Park,Hae Yong Kweon,Seong-Gon Kim
View all publications
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