Human TPFI2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human TPFI2 in Citrate plasma, Cell culture supernatant, Serum samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 56.25 - 3600 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 16 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.
Alternative names
Tissue factor pathway inhibitor 2, TFPI-2, Placental protein 5, PP5, TFPI2
What's included?
Recommended products
Human TPFI2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human TPFI2 in Citrate plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 56.25 - 3600 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 16 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95 | Range 88 - 101 % |
Sample type Serum | Average % = 103 | Range 96 - 113 % |
Sample type Citrate plasma | Average % = 96 | Range 88 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human TFPI2 ELISA kit (ab275908) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TFPI2 protein in human serum, plasma-citrate, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TFPI2 with 16 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
TFPI2, Tissue factor pathway inhibitor 2 also known as placental protein 5 (PP5), is a serine protease inhibitor that plays a key role in plasmin-mediated remodeling of the matrix by inhibiting trypsin and plasmin. TFPI2 is involved in matrix homeostasis, fetal development, and preventing tumor metastasis through invasion. Hypermethylation of this protein is associated with many cancer types, including, colorectal, pancreatic, and cervical cancers. The immunogen used to create the antibodies for this kit represents the mature form of the protein. The protein homologs in mouse in rat share little sequence homology, and as such, are not expected to cross-react with this kit.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Human TPFI2 ELISA Kit (ab275908)
Example of human TFPI2 standard curve in Sample Diluent NS.
The TFPI2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human TPFI2 ELISA Kit (ab275908)
Interpolated concentrations of native TFPI2 in human serum, plasma and cell culture supernatant samples.
The concentrations of TFPI2 were measured in duplicates, interpolated from the TFPI2 standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant serum 1:100, pregnant plasma (citrate) 1:100, and HeLa cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI2 concentration was determined to be 294.3 ng/mL in pregnant serum, 347 ng/mL in pregnant plasma (citrate), and 1.6 ng/mL in HeLa cell culture supernatant.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com