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AB314709

Human TPSB2 ELISA Kit

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Human TPSB2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TPSB2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

TPS2, TPSB2, Tryptase beta-2, Tryptase-2, Tryptase II

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Sandwich ELISA - Human TPSB2 ELISA Kit (AB314709)
  • sELISA

Supplier Data

Sandwich ELISA - Human TPSB2 ELISA Kit (AB314709)

Example of standard curve using ab314709. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2 ng/mL

Range

2 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TPSB2 (Tryptase beta-2) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TPSB2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human TPSB2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "69 - 142 %", "average": "= 102.1" }, { "sample": "Plasma", "range": "83 - 149 %", "average": "= 89.25" }, { "sample": "Cell culture media", "range": "82 - 149 %", "average": "= 120.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314709-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB314709-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin", "number":"AB314709-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314709-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human TPSB2 Protein Standard", "number":"AB314709-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human TPSB2 Antibody-coated ELISA Plate", "number":"AB314709-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314709-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314709-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human TPSB2 Detection Antibody", "number":"AB314709-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314709-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB314709-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPSB2 also known as tryptase beta-2 is an enzymatic protein with substantial mechanical significance. It functions as a serine protease typically requiring activation by cleavage. The protein has an approximate mass of 30 kDa. TPSB2 is found mainly in mast cells and to a lesser extent in basophils suggesting its role in immune response. Expression of TPSB2 is not uniform across tissues reflecting its specialized functions.
Biological function summary

Tryptase beta-2 is involved in mediating allergic reactions and inflammation. It is part of a tetrameric complex that consists of identical subunits to perform its catalytic function efficiently. The activity of TPSB2 affects the degradation of extracellular matrix components during inflammatory responses. By modifying structural proteins TPSB2 can facilitate the passage of immune cells to sites of inflammation reinforcing its role in immune surveillance and defense.

Pathways

TPSB2 plays an important role within the immune response and inflammatory pathways. It is intricately connected to mast cell degranulation a process critical in initiating defense mechanisms. TPSB2 interacts with other proteins such as tryptase alpha/beta 1 (TPSAB1) to modulate cellular responses. Furthermore its presence in the kallikrein-kinin system emphasizes its role in modulating vascular inflammation and repair mechanisms.

TPSB2 has associations with allergic conditions like asthma and inflammatory diseases such as arthritis. Its activity influences the severity and progression of these conditions by promoting inflammatory processes. In asthma TPSB2 can contribute to airway remodeling through its interactions with other mast cell proteins like chymase augmenting inflammation and tissue changes. Understanding its mechanism in these diseases offers potential targets for therapeutic interventions.
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Product protocols

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Target data

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
See full target information TPSB2
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB2378

Anti-Mast Cell Tryptase antibody [AA1]

BOND RX™ Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

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Proteins & Peptides

AB134529

Recombinant Human Mast Cell Tryptase protein (denatured)

SDS-PAGE - Recombinant Human Mast Cell Tryptase protein (denatured) (AB134529)

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